A new technique for harvesting costal cartilage with minimum sacrifice at the donor site

Yotsuyanagi, Takatoshi; Mikami, Makoto; Yamauchi, M.; Higuma, Yuko; Urushidate, Satoshi; Ezoe, Kyouri

doi:10.1016/j.bjps.2005.04.049

Cited by 24 publications

(15 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Constructs made from these CCs were mechanically testable and manipulatable with surgical tools, which was not true of previous TMJ disc cell constructs 17, 21, 22. The improvements in translatability include the surgeons’ familiarity harvesting costal cartilage, limited complications of this surgery, and abundance of healthy tissue 23, 24…”

Section: Introductionmentioning

confidence: 99%

A comparison of primary and passaged chondrocytes for use in engineering the temporomandibular joint

Anderson

Athanasiou

2009

Archives of Oral Biology

View full text Add to dashboard Cite

Objective-This study examines the tissue engineering potential of passaged (P3) and primary (P0) articular chondrocytes (ACs) and costal chondrocytes (CCs) from skeletally-mature goats for use in the temporomandibular joint (TMJ).Design-These four cell types were assembled into scaffoldless tissue engineered constructs and cultured for 4 wks. The constructs were then tested for cell, collagen, and glycosaminoglycan (GAG) content with biochemical assays, and collagen types I and II with enzyme-linked immunosorbent assays. Constructs were also tested under tension and compression to determine biomechanical properties.Results-Both primary and passaged CC constructs had greater GAG/wet weight than AC constructs. Primary AC constructs had significantly less total collagen and contained no collagen type I. AC P3 constructs had the largest collagen I/collagen II ratio, which was also greater in passaged CC constructs relative to primary groups. Primary AC constructs were not mechanically testable, while passaged AC and CC constructs had significantly greater tensile properties than primary CC constructs. Conclusions-PrimaryCCs are considerably better than primary ACs and have potential use in tissue engineering when larger quantities of collagen type II are desired. The poor performance of the ACs, in this study, which contradicts the results seen with previous studies using immature bovine ACs, may thus be attributed to the animals' maturity. However, CC P3 cells appear particularly wellsuited for tissue engineering fibrocartilage of the TMJ due to the high quantity of collagen and GAG, and tensile and compressive mechanical properties.

show abstract

Section: Introductionmentioning

confidence: 99%

A comparison of primary and passaged chondrocytes for use in engineering the temporomandibular joint

Anderson

Athanasiou

2009

Archives of Oral Biology

View full text Add to dashboard Cite

show abstract

“…By using autologous tissue, complications with patient rejection are eliminated. The costal cartilage can be harvested easily, and donor site morbidity is largely eliminated with current surgical techniques 12. A common problem with this approach in the jaw is overgrowth of the costal cartilage in the TMJ, which can require further surgery 13–16.…”

Section: Introductionmentioning

confidence: 99%

Passaged Goat Costal Chondrocytes Provide a Feasible Cell Source for Temporomandibular Joint Tissue Engineering

Anderson

Athanasiou

2008

Ann Biomed Eng

View full text Add to dashboard Cite

Costal cartilage is commonly harvested for various types of facial reconstructive surgery. The ability of costal chondrocytes (CCs) to produce relevant extracellular matrix, including glycosaminoglycans (GAGs), collagen type I, and collagen type II, makes them an appealing cell source for fibrocartilage tissue engineering. In order to obtain enough cells for tissue engineering, however, cell expansion will likely be necessary. This study examined CCs at passages 0, 1, 3, and 5, as well as temporomandibular (TMJ) disc cells, in a scaffoldless tissue engineering approach. TMJ disc constructs had over twice the collagen content of any other group, as well as the largest tensile properties; however, the substantial contraction of the constructs and limited cell numbers make it a non-feasible cell source for tissue engineering. In general, statistical differences in mechanical properties or total collagen content of the various CC groups were not observed; however, significantly more GAG was produced in the passaged CCs than the primary CCs. More collagen type II was also observed in some of the passaged cell groups than in passage 0. These results suggest not only feasibility but potential superiority of passaged CCs over primary CCs, which may lead to a functional engineered fibrocartilage tissue.

show abstract

“…The mean score of pliability and itching was 2.1 and 0.4, respectively. Patient satisfaction was also assessed based on a 5-point scale from very unsatisfied (0) to very satisfied (5). The mean satisfaction obtained at 2 months after surgery was 3.8.…”

Section: Resultsmentioning

confidence: 99%

“…Skin congestion and necrosis on the wound margin can occur after surgery, resulting in delayed wound healing, widening, or hypertrophic scarring. 5 The authors describe a new technique for costal cartilage harvesting without any scarring on the chest wall. The natural skin creases and the concavity of the umbilicus hide the surgical scar well.…”

Section: Discussionmentioning

confidence: 99%

Transumbilical Endoscopic Costal Cartilage Harvesting

Ching

Hsiao

2014

Annals of Plastic Surgery

View full text Add to dashboard Cite

show abstract

A new technique for harvesting costal cartilage with minimum sacrifice at the donor site

Cited by 24 publications

References 8 publications

A comparison of primary and passaged chondrocytes for use in engineering the temporomandibular joint

A comparison of primary and passaged chondrocytes for use in engineering the temporomandibular joint

Passaged Goat Costal Chondrocytes Provide a Feasible Cell Source for Temporomandibular Joint Tissue Engineering

Transumbilical Endoscopic Costal Cartilage Harvesting

Contact Info

Product

Resources

About