A new technique for quantitative bacterial assessment on burn wounds by modified dermabrasion

Pallua, Norbert; Fuchs, Paul C.; Hafemann, B.; Völpel, U; Noah, Mohamed S.; R, Lütticken

doi:10.1053/jhin.1998.0594

Cited by 15 publications

(11 citation statements)

References 11 publications

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“…Superficial, devitalized tissue removed by curettage, which is often used in the management of diabetic foot ulcers, may also be investigated for microbial content. Another technique involving dermabrasion has recently been described that enables the acquisition of deeper tissue without being as invasive as the biopsy method (181).…”

Section: Wound-sampling Methodsmentioning

confidence: 99%

“…Raahave et al (196), using a velvet pad surface imprint technique, reported that the median infective dose of mixed aerobes and anaerobes in postsurgical wounds was 4.6 ϫ 10 5 CFU/cm 2 , and Majewski et al (150), using a surface swab method, demonstrated that skin grafting was more successful in patients with wound contamination of Ͻ5 ϫ 10 4 CFU/cm 2 . A dermabrasion technique, considered to quantify tissue colonization while minimizing the degree of tissue invasion in burn wounds, has been shown to be more sensitive, both qualitatively and quantitatively, than a surface sample procedure (181). However, the technique requires specialized equipment, and the work of Pallua et al (181) excluded investigation for anaerobic bacteria.…”

Section: Significance Of Microorganisms In Woundsmentioning

confidence: 99%

“…A dermabrasion technique, considered to quantify tissue colonization while minimizing the degree of tissue invasion in burn wounds, has been shown to be more sensitive, both qualitatively and quantitatively, than a surface sample procedure (181). However, the technique requires specialized equipment, and the work of Pallua et al (181) excluded investigation for anaerobic bacteria.…”

Section: Significance Of Microorganisms In Woundsmentioning

confidence: 99%

“…One school of thought is that the density of microorganisms is the critical factor in determining whether a wound is likely to heal (100,102,151,196,202). However, a second school of thought argues that the presence of specific pathogens is of primary importance in delayed healing (59,130,149,181,216,217), while yet others have reported microorganisms to be of minimal importance in delayed healing (4,70,80,95,98,214,237).…”

Section: Introductionmentioning

confidence: 99%

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Wound Microbiology and Associated Approaches to Wound Management

2001

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The majority of dermal wounds are colonized with aerobic and anaerobic microorganisms that originate predominantly from mucosal surfaces such as those of the oral cavity and gut. The role and significance of microorganisms in wound healing has been debated for many years. While some experts consider the microbial density to be critical in predicting wound healing and infection, others consider the types of microorganisms to be of greater importance. However, these and other factors such as microbial synergy, the host immune response, and the quality of tissue must be considered collectively in assessing the probability of infection. Debate also exists regarding the value of wound sampling, the types of wounds that should be sampled, and the sampling technique required to generate the most meaningful data. In the laboratory, consideration must be given to the relevance of culturing polymicrobial specimens, the value in identifying one or more microorganisms, and the microorganisms that should be assayed for antibiotic susceptibility. Although appropriate systemic antibiotics are essential for the treatment of deteriorating, clinically infected wounds, debate exists regarding the relevance and use of antibiotics (systemic or topical) and antiseptics (topical) in the treatment of nonhealing wounds that have no clinical signs of infection. In providing a detailed analysis of wound microbiology, together with current opinion and controversies regarding wound assessment and treatment, this review has attempted to capture and address microbiological aspects that are critical to the successful management of microorganisms in wounds

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Section: Wound-sampling Methodsmentioning

confidence: 99%

Section: Significance Of Microorganisms In Woundsmentioning

confidence: 99%

Section: Significance Of Microorganisms In Woundsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Wound Microbiology and Associated Approaches to Wound Management

2001

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“…However, in these two studies, the wounds were sampled using swabs, which frequently identify the contaminant microorganisms on the wound surface, such as Staphylococcus spp. (Pallua et al 1999). Similarly, Mouro et al (2010) used swabs to collect sample from wounds during initial presentation, resulting in the isolation of mainly bacteria belonging to the cutaneous flora.…”

Section: Microbiological Analysismentioning

confidence: 99%

Deep tissue culture and hemoculture in dogs with wounds and sepsis

2017

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Bahr Arias M.V., Padilha F.N. & Perugini M.R.E. 2017. Deep tissue culture and hemoculture in dogs with wounds and sepsis. Pesquisa Veterinária Brasileira 37(12):1483-1490. Contaminated and infected wounds occur very frequently in veterinary medicine and can cause systemic inflammatory response syndrome, sepsis, and death. This study aimed to test the feasibility of collecting wound material by deep-tissue or punch biopsy for microbial culture, determine the frequency of bacteria in the wound(s) and blood cultures and the susceptibility of these microbes to antimicrobials, and evaluate clinical parameters that could be related to prognosis. Thirty dogs with wounds and signs of SIRS/sepsis were included in this study. Bacteria were isolated from all wounds and 41 bacterial isolates could be identified based on culture of the materials collected by punch biopsy; 53.66% of the isolates were gram-negative, mainly involving Pseudomonas aeruginosa, Klebsiella pneumoniae, and Enterococcus spp., and 46.34% were gram-positive bacteria such as Streptococcus spp., Enterococcus spp., and Staphylococcus spp. The survival rate was 66.67%. Based on blood culture analysis, we identified bacteremia in seven patients, predominantly of gram-negative bacteria, which negatively affected patient survival, as six dogs died. Hypoglycemia (≤60mg/dL) and severe hyperglycemia (≥180mg/dL) also negatively affected survival as 23.33% of the hypo/hyperglycemic dogs died. Factors such as blood lactate level at admission and hematocrit levels, and mean arterial pressure were not significantly correlated with death or survival of the dogs.

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Measurements in Burns

McKinnell

Pape

2012

Measurements in Wound Healing

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A new technique for quantitative bacterial assessment on burn wounds by modified dermabrasion

Cited by 15 publications

References 11 publications

Wound Microbiology and Associated Approaches to Wound Management

Wound Microbiology and Associated Approaches to Wound Management

Deep tissue culture and hemoculture in dogs with wounds and sepsis

Measurements in Burns

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