A new technique of enzyme entrapment for planar biosensors

Varlan, Anca; Suls, Jan; Jacobs, P; Sansen, Willy

doi:10.1016/0956-5663(95)96967-4

Cited by 25 publications

(4 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, magnetic particles with immobilized immunoreagents are used with increasing frequency in immunoassay manual methods. , This approach simplifies the renewal of the solid phase. Several reports have appeared in recent years where biologically modified magnetic particles are used in conjunction with analytical flow systems. ,− These particles are handled easily and they make the regeneration procedures of the biological material unnecessary.…”

mentioning

confidence: 99%

Flow Injection Immunoanalysis Based on a Magnetoimmunosensor System

et al. 1998

View full text Add to dashboard Cite

A new immunosensor integrated to a flow system has been developed. It is based on magnetic immunoparticles immobilized on a solid-state transducer using a magnetic field. The described technique renews the immunoparticles reproducibly for each analysis allowing a good measurement precision. The developed experimental approach permits the implementation of an automated immunoassay that is quick (analytical cycle < 30 min) and sensitive in the micromolar concentration range. The system was applied to the determination of rabbit immunoglobulin G as an analyte model.

show abstract

mentioning

confidence: 99%

Flow Injection Immunoanalysis Based on a Magnetoimmunosensor System

et al. 1998

View full text Add to dashboard Cite

show abstract

“…The use of magnetic beads as an alternative support for the immobilization of biomolecules was reported for the first time by Varlan and et al [20]. In this paper, a glucose oxidase immunoconjugate was left to incubate with modified magnetic beads; then, they were fixed on a sensor by a thick-film permanent magnet localized in the back side of the sensor.…”

Section: Magnetic Beads As a Solid Phase For Immunoassaymentioning

confidence: 93%

Electrochemical arrays coupled with magnetic separators for immunochemistry

2010

View full text Add to dashboard Cite

Electrochemical methods are increasingly applied to immunoassays, because they overcome problems associated with other modes of detection. In particular, with respect to conventional immunoassays, electrochemical immunosensors show versatility, reliability, and fast analysis time. In immunosensor strategy, the antigen or antibody can be immobilized directly onto the surface of the electrochemical transducer that will finally be used to reveal the amount of the affinity reaction. However, the use of the electrode surface as a solid phase as well as an electrochemical transducer presents some problems: a shielding of the surface by biospecifically bound antibody molecules can cause hindrance in the electron transfer, resulting in a reduced voltammetric signal. Thus, as an alternative solid phase, magnetic beads because of their low toxicity and high biocompatibility have gained much attention in chemistry, associated with various analytical techniques, due to their suitability for immobilization of biomolecules. Magnetic micro-or nanobeads can be separated easily and quickly by magnetic forces and will be used together with bioaffine ligands, e.g., antibodies or proteins with a high affinity to the target. The special advantages of magnetic separation techniques are the fast and simple handling of a sample vial and the opportunity to deal with large sample volumes without the need for timeconsuming centrifugation steps. This also makes biomagnetic separation ideal for automated assay/analysis systems which will play a very important role in the near future. This review presents some examples of immunochemical assay developed using magnetic beads as a solid phase coupled with electrochemical detection techniques, in particular, using electrochemical arrays as transducers. Applications related to static measurements, together with in-flow detection systems are presented.

show abstract

“…Latex beads, containing superparamagnetic material covalently modified with enzymes, have been patterned on a transducer by the means of screen printed thick films permanent magnets. Such a method, valuable for batch production independently of the nature of the substrate material, was evaluated for assessing glucose concentrations up to 20mM [ 32 ].…”

Section: Enzymatic Biosensors Based Onto Magnetic Microbeadsmentioning

confidence: 99%

Biosensors and Bio-Bar Code Assays Based on Biofunctionalized Magnetic Microbeads

Jaffrézic‐Renault

Martelet

Chevolot

et al. 2007

Sensors

117

View full text Add to dashboard Cite

This review paper reports the applications of magnetic microbeads in biosensors and bio-bar code assays. Affinity biosensors are presented through different types of transducing systems: electrochemical, piezo electric or magnetic ones, applied to immunodetection and genodetection. Enzymatic biosensors are based on biofunctionalization through magnetic microbeads of a transducer, more often amperometric, potentiometric or conductimetric. The bio-bar code assays relie on a sandwich structure based on specific biological interaction of a magnetic microbead and a nanoparticle with a defined biological molecule. The magnetic particle allows the separation of the reacted target molecules from unreacted ones. The nanoparticles aim at the amplification and the detection of the target molecule. The bio-bar code assays allow the detection at very low concentration of biological molecules, similar to PCR sensitivity.

show abstract

A new technique of enzyme entrapment for planar biosensors

Cited by 25 publications

References 4 publications

Flow Injection Immunoanalysis Based on a Magnetoimmunosensor System

Flow Injection Immunoanalysis Based on a Magnetoimmunosensor System

Electrochemical arrays coupled with magnetic separators for immunochemistry

Biosensors and Bio-Bar Code Assays Based on Biofunctionalized Magnetic Microbeads

Contact Info

Product

Resources

About