A new type of homodiploid fish derived from the interspecific hybridization of female common carp × male blunt snout bream

Wang, Shi; Ye, Xiaolan; Wang, Yude; Chen, Yuting; Lin, Bowen; Yi, Zhenfeng; Mao, Zhuangwen; Hu, Fangzhou; Zhao, Rurong; Wang, Juan; Zhou, Rong; Ren, Li; Yao, Zhanzhou; Tao, Min; Chun, Zhang; Xiao, Jun; Qin, Qinbo; Liu, Shaojun

doi:10.1038/s41598-017-04582-z

Cited by 32 publications

(15 citation statements)

References 34 publications

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“…To determine the ploidy of GGC, KOC and GC, chromosome counting were performed using kidney tissues from 20 GGC, 20 KOC and 20 GC at age of 6 months. The chromosomes were prepared in accordance to the method described by Wang et al [11]. After culturing for two to 3 days at 20–22 °C, the samples were injected one to three times with concanavalin A at a dose of 6–15 μg/g body weight at an interval of 12–24 h. Two to 3 h prior to dissection, each sample was injected with colchicine at a dose of 4–6 μg/g body weight.…”

Section: Methodsmentioning

confidence: 99%

Evidence for paternal DNA transmission to gynogenetic grass carp

Mao

Wang

et al. 2019

BMC Genet

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BackgroundGrass carp (Ctenopharyngodon idellus, GC), as the highest-output fish in China, is economically important. The production of gynogenetic grass carp (GGC) will provide important germplasm resource for producing improved GC. At present, knowledge regarding the heterologous sperm DNA in gynogenetic offspring is little. Thus, revealing paternal DNA in GGC at the molecular level would be highly significant for fish genetic breeding.ResultIn this study, ultraviolet-treated sperm of koi carp (Cyprinus carpio haematopterus, KOC, 2n = 100), was used to activate the eggs of GC (2n = 48). Afterwards, cold shock (0–4 °C) was administered for 12 min to double the chromosomes, resulting in GGC. No significant difference (p > 0.05) was found between GGC and GC in appearance, erythrocytes size and chromosome numbers. However, at the molecular level, a specific microsatellite DNA fragment (MFW1-gynogenetic grass carp, MFW1-G) derived from the paternal parent KOC was found to be transmitted into GGC.ConclusionsFor the first time, this study provided an evidence at the molecular level that the DNA fragment derived from the paternal parent occurred in GGC. This finding is of great significance for fish genetic breeding.

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Section: Methodsmentioning

confidence: 99%

Evidence for paternal DNA transmission to gynogenetic grass carp

Mao

Wang

et al. 2019

BMC Genet

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“…All of the natural materials, such as common carp ( Cyprinus carpio , 2n = 100, abbreviated as COC) and blunt snout bream ( Megalobrama amblycephala , 2n = 48, abbreviated as BSB) were obtained from the Center for Polyploidy Fish Genetics Breeding of Hunan Province located at Hunan Normal University, Changsha, Hunan, China. The protocols for crossing and culturing were described previously [23]. The two types of improved carp offspring from COC (♀) × BSB (♂) were the improved diploid common carp (2n = 100, IDC-F 1 ) and the improved diploid scattered mirror carp (2n = 100, IDMC-F 1 ); the phenotype of the latter has changed significantly from that of the female parent, COC.…”

Section: Methodsmentioning

confidence: 99%

“…In our previous study, we successfully obtained two types of improved carp offspring from common carp (2n = 100, abbreviated COC) (♀) × blunt snout bream (2n = 48, abbreviated BSB) (♂); one is the improved diploid common carp (2n = 100, IDC-F 1 ), and the other is the improved diploid scattered mirror carp (2n = 100, IDMC-F 1 ) [23]. In this study, we carried out self-crossing of these two types of improved carp offspring (IDC-F 1 and IDMC-F 1 ), respectively.…”

Section: Introductionmentioning

confidence: 99%

Rapid genomic DNA variation in newly hybridized carp lineages derived from Cyprinus carpio (♀) × Megalobrama amblycephala (♂)

Luo

Wang

et al. 2019

BMC Genet

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BackgroundDistant hybridization can generate changes in phenotypes and genotypes that lead to the formation of new hybrid lineages with genetic variation. In this study, the establishment of two bisexual fertile carp lineages, including the improved diploid common carp (IDC) lineage and the improved diploid scattered mirror carp (IDMC) lineage, from the interspecific hybridization of common carp (Cyprinus carpio, 2n = 100) (♀) × blunt snout bream (Megalobrama amblycephala, 2n = 48) (♂), provided a good platform to investigate the genetic relationship between the parents and their hybrid progenies.ResultIn this study, we investigated the genetic variation of 12 Hox genes in the two types of improved carp lineages derived from common carp (♀) × blunt snout bream (♂). Hox gene clusters were abundant in the first generation of IDC, but most were not stably inherited in the second generation. In contrast, we did not find obvious mutations in Hox genes in the first generation of IDMC, and almost all the Hox gene clusters were stably inherited from the first generation to the second generation of IDMC. Interestingly, we found obvious recombinant clusters of Hox genes in both improved carp lineages, and partially recombinant clusters of Hox genes were stably inherited from the first generation to the second generation in both types of improved carp lineages. On the other hand, some Hox genes were gradually becoming pseudogenes, and some genes were completely pseudogenised in IDC or IDMC.ConclusionsOur results provided important evidence that distant hybridization produces rapid genomic DNA changes that may or may not be stably inherited, providing novel insights into the function of hybridization in the establishment of improved lineages used as new fish resources for aquaculture.

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“…The reunion of two divergent genomes in a common nucleus during allopolyploid speciation can lead to immediate and profound genome modifications, such as sequence elimination [ 9 – 13 ], epigenetic diversity [ 14 – 16 ], activation of genes and retroelements [ 17 ], and homoeologous interactions and exchanges [ 18 , 19 ]. Notably, these modifications may directly or indirectly contribute to the establishment of a nascent polyploid and its evolutionary success as a new species [ 20 ]. In addition, the reconciliation of divergent parental genomes and regulatory interactions can be due to the initiation of hybrid heterosis [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Asymmetric expression patterns reveal a strong maternal effect and dosage compensation in polyploid hybrid fish

Liu

Tan

et al. 2018

BMC Genomics

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BackgroundHybridization and polyploidization are regarded as the major driving forces in plant speciation, diversification, and ecological adaptation. Our knowledge regarding the mechanisms of duplicated-gene regulation following genomic merging or doubling is primarily derived from plants and is sparse for vertebrates.ResultsWe successfully obtained an F1 generation (including allodiploid hybrids and triploid hybrids) from female Megalobrama amblycephala Yih (BSB, 2n = 48) × male Xenocypri davidi Bleeker (YB, 2n = 48). The duplicated-gene expression patterns of the two types of hybrids were explored using RNA-Seq data. In total, 5.44 × 108 (69.32 GB) clean reads and 499,631 assembled unigenes were obtained from the testis transcriptomes. The sequence similarity analysis of 4265 orthologs revealed that the merged genomes were dominantly expressed in different ploidy hybrids. The differentially expressed genes in the two types of hybrids were asymmetric compared with those in both parents. Furthermore, the genome-wide expression level dominance (ELD) was biased toward the maternal BSB genome in both the allodiploid and triploid hybrids. In addition, the dosage-compensation mechanisms that reduced the triploid expression levels to the diploid state were determined in the triploid hybrids.ConclusionsOur results indicate that divergent genomes undergo strong interactions and domination in allopolyploid offspring. Genomic merger has a greater effect on the gene-expression patterns than genomic doubling. The various expression mechanisms (including maternal effect and dosage compensation) in different ploidy hybrids suggest that the initial genomic merger and doubling play important roles in polyploidy adaptation and evolution.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-4883-7) contains supplementary material, which is available to authorized users.

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A new type of homodiploid fish derived from the interspecific hybridization of female common carp × male blunt snout bream

Cited by 32 publications

References 34 publications

Evidence for paternal DNA transmission to gynogenetic grass carp

Evidence for paternal DNA transmission to gynogenetic grass carp

Rapid genomic DNA variation in newly hybridized carp lineages derived from Cyprinus carpio (♀) × Megalobrama amblycephala (♂)

Asymmetric expression patterns reveal a strong maternal effect and dosage compensation in polyploid hybrid fish

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