A newly discovered class of human hematopoietic cells with SCID-repopulating activity

Bhatia, Mickie; Bonnet, Dominique; Murdoch, Barbara; Gan, Olga I.; Dick, John E.

doi:10.1038/2023

Cited by 570 publications

(471 citation statements)

References 34 publications

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“…The absence of expression on these cells was not dependent on the epitope recognized by the CD150 mAb used in this study, because five other CD150 mAbs were tested and all yielded identical results (Table 4). This finding does not exclude the possibility that CD150 is expressed on a very small subset of CD133 − CD34 − HSCs [20]. Analysis of CD150 on cytokine-mobilized PBLs before CD133 magnetic selection showed that 1.64% of CD34 − CD45 + Lin − cells expressed CD150 (data not shown).…”

Section: Resultsmentioning

confidence: 94%

Differential expression of CD150 (SLAM) family receptors by human hematopoietic stem and progenitor cells

Sintes

Romero

Marı́n³

et al. 2008

Experimental Hematology

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Objectives-Human hematopoietic stem cell (HSC)-containing grafts are most commonly used to treat various blood diseases, including leukemias and autoimmune disorders. CD150 (SLAM) family receptors have recently been shown to be differentially expressed by mouse HSC and progenitor cells. Members of the CD150 family are key regulators of leukocyte activation and differentiation. The goal of the present study is to analyze the expression patterns of the CD150 receptors CD48, CD84, CD150 (SLAM), CD229 (Ly9), and CD244 (2B4) on the different sources of human hematopoietic stem and progenitor cells.Materials and Methods-Expression of CD150 receptors was analyzed on human mobilized peripheral blood CD133 + -isolated cells and CD34 + bone marrow (BM) and umbilical cord blood (CB) cells using multicolor flow cytometry.Results-CD244 was present on most CD133 + Lin − -mobilized cells and CD34 + Lin − BM and CB cells, including virtually all CD38 − Lin − primitive progenitor cells. CD48 had a restricted expression pattern on CD133 + Lin − CD38 − cells, while its levels were significantly higher in CD34 + Lin − BM and CB cells. In addition, CD84 was present on a significant number of CD133 + Lin − cells, but only on a small fraction of CD133 + Lin − CD38 − peripheral blood mobilized cells. In contrast, CD84 was expressed on practically all CD34 + Lin − BM cells. No CD150 expression was observed in mobilized peripheral blood CD133 + Lin − or CD34 + Lin − BM and CB cells. Furthermore, only a small fraction of CD34 + Lin − BM and CB cells expressed CD229.Conclusions-Our results show that CD150 family molecules are present on human hematopoietic stem and progenitor cells and that their expression patterns differ between humans and mice.Hematopoietic stem cells (HSCs) are a rare cell type found mainly in the bone marrow (BM), which have the capacity to self-renew and differentiate into all blood cell lineages [1].

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Section: Resultsmentioning

confidence: 94%

Differential expression of CD150 (SLAM) family receptors by human hematopoietic stem and progenitor cells

Sintes

Romero

Marı́n³

et al. 2008

Experimental Hematology

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“…Recent reports have also suggested that human CD34 -cells in CB and BM cells contain in vivo hematopoietic repopulating cells in NOD/SCID mice [23] and in sheep [38]. These observations led us to study whether the fractions of CD34 -cells in CB may contain SRCs.…”

Section: Engraftment Of Cd34 -Cells From Human Cb Cells In Nod/scid Micementioning

confidence: 98%

“…However, the phenotype of SRCs was not elucidated in this study. More recently, using the same NOD/SCID xenograft system, they demonstrated that SRCs are also present in the CD34 -fraction of human CB cells [23].…”

Section: Introductionmentioning

confidence: 99%

Comparison of Hematopoietic Activities of Human Bone Marrow and Umbilical Cord Blood CD34 Positive and Negative Cells

et al. 1999

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“…The frequency of human and murine hematopoietic stem cells has been estimated to be 1/10 4 -10 5 bone marrow cells (Harrison et al, 1989;Sutherland et al, 1989;Harrison, 1993;Szilvassy and Hoffman, 1995;Bhatia et al, 1998;Abkowitz et al, 2002). The rarity of this population presented considerable impediments to isolation until recently.…”

Section: Methods Of Identifying Hematopoietic Stem and Progenitor Cellsmentioning

confidence: 99%

Blood cell progenitors: Insights into the properties of stem cells

Yöder

2003

Anat. Rec.

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Hematopoiesis is a dynamic process in which eight lineages of mature blood cells are derived from a common stem cell. Great progress has been made in identifying the functionally disparate progenitors that emerge from the stem cell and in elucidating the molecules required for their growth and survival. Further work will be required to understand the molecular mechanisms that regulate commitment of stem and progenitor cells to each stage of progenitor cell development and ultimately into the mature blood cells. Anat Rec Part A 276A: 66 -74, 2004.

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A newly discovered class of human hematopoietic cells with SCID-repopulating activity

Cited by 570 publications

References 34 publications

Differential expression of CD150 (SLAM) family receptors by human hematopoietic stem and progenitor cells

Differential expression of CD150 (SLAM) family receptors by human hematopoietic stem and progenitor cells

Comparison of Hematopoietic Activities of Human Bone Marrow and Umbilical Cord Blood CD34 Positive and Negative Cells

Blood cell progenitors: Insights into the properties of stem cells

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