A Newly Identified Oral Mycoplasma (M. Orale) and Its Relationship to Other Human Mycoplasmas

Taylor‐Robinson, David; Canchola, Jose G.; Fox, H.; Chanock, Robert M.

doi:10.1093/oxfordjournals.aje.a120454

Cited by 44 publications

(48 citation statements)

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“…This finding suggests that the oral anaerobic mycoplasmas may be genetically related to each other, since the composition of cell proteins may reflect genetic relatedness. Complementfixation tests showed a high level one way cross reaction between M. salivarium and M. oraZe (Taylor-Robinson et al 1964). On the other hand, the few available results of nucleic acid homology determinations showed only a low degree of cross-reaction between the anaerobic oral mycoplasmas .…”

Section: Discussionmentioning

confidence: 94%

“…orale type I is also a common inhabitant of the oropharynx; less common are M. orale types 2 and 3 and M . hominis type I (Taylor-Robinson, Canchola, Fox & Chanock, 1964;TaylorRobinson, Fox & Chanock, 1965;Purcell & Chanock, 1967). The relationship of these strains to respiratory tract diseases has been investigated, but there is as yet no con-clusive evidence that they are able to produce disease in man (Glezen, Thornburg, Chin & Wenner, 1967).…”

Section: Introductionmentioning

confidence: 99%

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Identification of Mycoplasmas of Human Origin

Haas

Sacks

Razin

1968

Journal of General Microbiology

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S U M M A R YFifty-five strains of the genus Mycoplasma were isolated from sputam of 95 patients suffering from various respiratory tract diseases. The polyacrylamide gel electrophoretic method was used to identify the isolated strains, in addition to the conventional biochemical methods and growth-inhibition by antisera. The electrophoretic patterns of the isolated strains were compared with those of known human mycoplasmas. The electrophoretic patterns obtained were species specific and highly reproducible. The results of the identification of the isolated strains by the gel electrophoretic method correlated well with those of the growth inhibition test. It is suggested that polyacrylamide gel electrophoresis of whole cell proteins might become a useful routine method for identification of mycoplasmas.The most prevalent Mycoplasma found in the sputa was Mycoplasma salivarium, only four strains were identified as M . orale type I and one strain as M. pneumoniae. One strain was similar to, but not identical with, M. salivarium. The three oral anaerobic mycoplasmas (M. salivarium, M . orale types I and 2) showed a certain similarity when examined by the gel electrophoretic method, they may be genetically related to each other.

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Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Identification of Mycoplasmas of Human Origin

Haas

Sacks

Razin

1968

Journal of General Microbiology

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“…Eight units of antigen were used and the tests were performed by overnight fixation at 40 C. in micro CF plates. The method used for IHA was that described previously (Taylor-Robinson, Canchola, Fox & Chanock, 1964) but modified by the use of sensitized sheep erythrocytes stored at -700 C. (Taylor-Robinson, Ludwig, Purcell, Mufson & Chanock, 1965). The TRI test for measurement of antibody to M. pneumoniae was essentially that described by Jensen (1964).…”

Section: Mycoplasmasmentioning

confidence: 99%

“…In addition, rabbit antisera to M. hominis type 1, M. hominis type 2, M. salivarium, M. orale, the Navel strain of mycoplasma and a newly identified species from the human oropharynx, strain CH 20247 (Taylor-Robinson, Fox & Chanock, 1965), each at a dilution of 1/10, did not inhibit the growth of the three mycoplasma species studied. Although the homologous growth-inhibition titre of each of the last six antisera was unknown, they all possessed high levels of complement-fixing and indirect-haemagglutination antibodies and they all produced zones of inhibition in the disk growth-inhibition test (Taylor-Robinson et al 1964;Taylor-Robinson, Fox & Chanock, 1965). To.…”

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confidence: 99%

A colour test for the measurement of antibody to certain mycoplasma species based upon the inhibition of acid production

Taylor‐Robinson

Purcell

Wong

et al. 1966

J. Hyg.

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293

119

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A property of mycoplasmas (PPLO) is the inhibition of their growth by specific antibody. Edward & Fitzgerald (1954) Jensen (1964). This is based upon the inhibition of reduction of tetrazolium. Unfortunately, this technique does not have wide application since M. pneumoniae is unique among the mycoplasma species that infect man in its ability to reduce readily tetrazolium salts. However, the growth of M. pneumoniae and certain other mycoplasma species is accompanied by the production of acid, and the incorporation of phenol red into the medium permits the resultant pH change to be seen. The addition of specific antiserum to the medium inhibits mycoplasma growth and this inhibition can be visualized by failure of the indicator to change colour. This communication describes the development of this technique for the quantitative measurement of growth-inhibiting antibody to the acidproducing mycoplasmas and indicates its practical significance.

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“…A second antigenic type of oral Mycoplasma was identified and given the name of Mycoplasma orale by Herderschee, Ruys & van Rhijn (1963) and by Taylor-Robinson, Canchola, Fox & Chanock (1964).…”

Section: Introductionmentioning

confidence: 99%

Lipolytic Activity by Oral Pleuropneumonia-Like (Mycoplasma) Organisms

Cole

Pease

1967

Journal of General Microbiology

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SUMMARYNinety-five strains of Mycoplasma were isolated from human saliva; all were micro-aerophilic ; 72 were actively lipolytic and 23 were non-lipolytic and were inhibited by Tweens. A type culture of human type 4 (Mycoplasma salivarum) was culturally identical with the lipolytic strains, and two type cultures of M. orale with the non-lipolytic strains. The strains serologically identifiable as M. orale were non-lipolytic, whereas those identifiable as M. salivarium were of both types. Comparison was also made with type strains from other sources. Those of human origin were non-lipolytic and those of saprophytic or animal origin were slightly lipolytic.

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A Newly Identified Oral Mycoplasma (M. Orale) and Its Relationship to Other Human Mycoplasmas

Cited by 44 publications

References 0 publications

Identification of Mycoplasmas of Human Origin

Identification of Mycoplasmas of Human Origin

A colour test for the measurement of antibody to certain mycoplasma species based upon the inhibition of acid production

Lipolytic Activity by Oral Pleuropneumonia-Like (Mycoplasma) Organisms

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