A newly isolated Bacillus siamensis SB1001 for mass production of poly-γ-glutamic acid

Wang, Dexin; Hwang, Jinsu; Kim, Dongho; Lee, Sungbeom; Kim, Dae-Hyuk; Joe, Min-Ho

doi:10.1016/j.procbio.2019.11.034

Cited by 34 publications

(30 citation statements)

References 38 publications

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“…The 16S rDNA gene of the isolated strains was amplified, sequenced, and analyzed as previously described [23]. The phylogenetic tree was constructed using the neighbor-joining method in MEGA 7.0.…”

Section: Methodsmentioning

confidence: 99%

“…The biomass was determined according to the standard calibration curve between the OD 600 and dry cell weight (DCW) by measuring the OD 600 of the broth [23].…”

Section: Methodsmentioning

confidence: 99%

“…γ-PGA was purified by the ethanol precipitation method as previously described [23]. The concentration of γ-PGA was determined by an Agilent 1100 high-performance liquid chromatography (HPLC) system using a PL aquagel-OH gel permeation chromatogram (GPC) column (300 × 7.5 mm;…”

Section: Methodsmentioning

confidence: 99%

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Simultaneous production of poly-γ-glutamic acid and 2,3-butanediol by a newly isolated Bacillus subtilis CS13

Wang¹,

Kim²,

Lee³

et al. 2020

Appl Microbiol Biotechnol

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Background: Bacillus subtilis naturally produces large amounts of 2,3-butanediol (2,3-BD) as the main byproduct during poly-γ-glutamic acid (γ-PGA) fermentation using carbon sources. 2,3-BD is a promising platform chemicals in various industries, and co-production has great economic benefits. Thus, co-production of poly-γ-glutamic acid (γ-PGA) and 2,3-butanediol (2,3-BD) by Bacillus subtilis were investigated for the first time. Results: In this study, a novel Bacillus subtilis CS13 was isolated that can efficiently co-production of γ-PGA and 2,3-BD. The fermentation medium and culture parameters by B. subtilis CS13 were optimized using statistical methods. It was observed that sucrose, L-glutamic acid, ammonium citrate, and MgSO 4 •7H 2 O were favorable for γ-PGA and 2,3-BD co-production at pH 6.5 and 37 °C. A medium composed of 119.83 g/L sucrose, 48.85 g/L L-glutamic acid, 21.08 g/L ammonium citrate, and 3.21 g/L MgSO 4 •7H 2 O was optimized by response surface methodology (RSM). The results show that the yields of γ-PGA and 2,3-BD reached 27.79 ± 0.87 g/L at 24 h and 57.05 ± 1.28 g/L at 84 h with the optimized medium, respectively. Conclusions: To our knowledge, the co-production of 2,3-BD and γ-PGA will reduce the costs of production and separation in theory and provide a new perspective for industrial production of γ-PGA and 2,3-BD. B. subtilis CS13 as a generally recognized as safe (GRAS) strain, has great promise for the co-production of 2,3-BD and γ-PGA.

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Section: Methodsmentioning

confidence: 99%

“…The biomass was determined according to the standard calibration curve between the OD 600 and dry cell weight (DCW) by measuring the OD 600 of the broth [23].…”

Section: Methodsmentioning

confidence: 99%

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Simultaneous production of poly-γ-glutamic acid and 2,3-butanediol by a newly isolated Bacillus subtilis CS13

Wang¹,

Kim²,

Lee³

et al. 2020

Appl Microbiol Biotechnol

Self Cite

View full text Add to dashboard Cite

show abstract

“…The cell biomass was determined by measuring the absorbance of the broth at 600 nm using a UV-Vis spectrophotometer (Libra S70PC, Biochrom Ltd., Cambridge, England) [23].…”

Section: Analysis Methodsmentioning

confidence: 99%

“…Bacillus siamensis SB1001 was isolated from organically cultivated soybeans and maintained in sterile glycerol (50%, w/v) at -80 °C [23]. The mutant strain B. siamensis IR10 was isolated in this study.…”

Section: Strains and Mediummentioning

confidence: 99%

High-level production of poly-γ-glutamic acid from untreated molasses by Bacillus siamensis IR10

et al. 2020

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Background: Poly-γ-glutamic acid (γ-PGA) is a promising biopolymer and has been applied in many fields. Bacillus siamensis SB1001 was a newly isolated poly-γ-glutamic acid producer with sucrose as its optimal carbon source. To improve the utilization of carbon source, and then molasses can be effectively used for γ-PGA production, 60 cobalt gamma rays was used to mutate the genes of B. siamensis SB1001. Results: Bacillus siamensis IR10 was screened for the production of γ-PGA from untreated molasses. In batch fermentation, 17.86 ± 0.97 g/L γ-PGA was obtained after 15 h, which is 52.51% higher than that of its parent strain. Fed-batch fermentation was performed to further improve the yield of γ-PGA with untreated molasses, yielding 41.40 ± 2.01 g/L of γ-PGA with a productivity of 1.73 ± 0.08 g/L/h. An average γ-PGA productivity of 1.85 g/L/h was achieved in the repeated fed-batch fermentation. This is the first report of such a high γ-PGA productivity. The analysis of the enzyme activities showed that they were affected by the carbon sources, enhanced ICDH and GDH, and decreased ODHC, which are important for γ-PGA production. Conclusion: These results suggest that untreated molasses can be used for economical and industrial-scale production of γ-PGA by B. siamensis IR10.

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Biopolymer and polymer precursor production by microorganisms: applications and future prospects

Saharan,

Kamal,

Badoni

et al. 2023

J of Chemical Tech & Biotech

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Polymer has been used in various industries over the past few decades due to its tremendous applications. Among these, polyhydroxyalkanoates (PHA) and polylactic acid (PLA), are easily biodegradable biopolymers derived from bacteria, including recombinant Escherichia coli, Alcaligenes eutrophus, Alcaligenes latus, Azotobacter vinelandii, methylotrophs, and Pseudomonas. Conventional petroleum‐derived polymers have become potentially harmful to the environment due to their complex degradation process. The non‐biodegradability of synthetic polymers has become a global issue of concern. There is an urgent need for a substitute to tackle the increasing environmental stress. Microorganisms are small factories for producing different types of polymers during their growth cycle. The various features like biodegradability, biocompatibility, non‐toxicity, and wide substrate spectrum make the microbial polymers highly reliable. Biopolymers such as alginate, cellulose, cyanophycin, levan, poly‐hydroxyalkanoates, xanthan, poly‐lactic acid, and poly‐γ‐glutamic acid can be obtained from different microorganisms like Aureobasdium pullulans, Acetobacter xylinum, Bacillus thermoamylovorans, Cupriavidusnecator. These are extensively used in various fields like food, medicine, wastewater treatment, biofuel production, packaging, and cosmetics. Despite being advantageous in several ways, the biopolymer market still faces several hurdles. This review mainly emphasizes the different types of biopolymers, production by microorganisms and various applications of these biopolymers in different fields. The main drawback limiting the development of these polymers is the high production cost and low efficiency of the microbial strains. Genetic recombination is an efficient technique to enhance the microbial yield and to expand the biopolymers market size.This article is protected by copyright. All rights reserved.

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A newly isolated Bacillus siamensis SB1001 for mass production of poly-γ-glutamic acid

Cited by 34 publications

References 38 publications

Simultaneous production of poly-γ-glutamic acid and 2,3-butanediol by a newly isolated Bacillus subtilis CS13

Simultaneous production of poly-γ-glutamic acid and 2,3-butanediol by a newly isolated Bacillus subtilis CS13

High-level production of poly-γ-glutamic acid from untreated molasses by Bacillus siamensis IR10

Biopolymer and polymer precursor production by microorganisms: applications and future prospects

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