A Newly Isolated Streptomyces sp. YYS-7 With a Broad-Spectrum Antifungal Activity Improves the Banana Plant Resistance to Fusarium oxysporum f. sp. cubense Tropical Race 4

Wei, Yuanhuan; Zhao, Yanbin; Zhou, Dengbo; Qi, Defeng; Li, Kai; Tang, Wen; Chen, Yufeng; Tao, Jing; Zang, Xiaoping; Xie, Jianghui; Wang, Wei

doi:10.3389/fmicb.2020.01712

Cited by 52 publications

(61 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The morphological characteristics of Foc TR4 mycelia treated with H3-2 extracts were assessed by scanning electron microscopy (SEM; TM4000Plus, Hitachi, Japan). The methods of sample preparation and analysis were referred to the publication with a slight modification ( Wei et al, 2020 ). A 5-mm-diameter disk of Foc TR4 was inoculated in the PDA plate containing 4 × EC 50 of strain H3-2 extracts.…”

Section: Methodsmentioning

confidence: 99%

A Novel Antifungal Actinomycete Streptomyces sp. Strain H3-2 Effectively Controls Banana Fusarium Wilt

et al. 2021

Self Cite

View full text Add to dashboard Cite

Banana Fusarium wilt disease caused by Fusarium oxyspoum f. sp. cubense (Foc) seriously threatens the banana industry. Foc tropical race 4 (Foc TR4) can infect almost all banana cultivars. Compared with traditional physical and chemical practices, biocontrol strategy using beneficial microbes is considered as an environmentally sound option to manage fungal disease. In this study, a strain, H3-2, isolated from a non-infected banana orchard, exhibited high antifungal activity against Foc TR4. According to its morphological, physiological, and biochemical characteristics, the strain H3-2 was identified as Streptomyces sp. and convinced by the polymorphic phylogenic analysis of 16S rRNA sequences. Extracts of the strain H3-2 suppressed the growth and spore germination of Foc TR4 in vitro by destroying cell membrane integrity and mycelial ultrastructure. Notably, the strain and its extracts showed broad-spectrum antifungal activity against the selected seven fungal phytopathogens. Fourteen chemical compounds in the extracts were identified by gas chromatography–mass spectrometer (GC-MS), primarily phenolic compounds. Additional pot inoculation experiment demonstrated that the fermentation broth of the strain H3-2 promoted the growth of banana seedlings by efficiently inhibiting the spread of banana Fusarium wilt disease. This study demonstrated the potential application of the novel Streptomyces sp. H3-2 for the management of banana Fusarium wilt.

show abstract

Section: Methodsmentioning

confidence: 99%

A Novel Antifungal Actinomycete Streptomyces sp. Strain H3-2 Effectively Controls Banana Fusarium Wilt

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Banana is an important food crop in the tropics and subtropics, and fusarium wilt is one of the most destructive diseases affecting agricultural production of this fruit. Biological control is a promising strategy for combating fusarium wilt, due to the advantages of high efficiency, broad spectrum, and the fact that it is relatively environmentally friendly ( Zhou et al, 2013 ; Wei et al, 2020 ). Previous studies have indicated that specific microbial groups, such as Pseudomonas , Streptomyces , Flavobacterium , etc., are closely related to plant disease suppression ( Mendes et al, 2011 ; Cha et al, 2016 ; Kwak et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

“…We did not find swollen mycelia in Foc TR4 treated with strain JBS5-6 extracts. This might be because the swollen morphology was an earlier symptom of abnormalities ( Wei et al, 2020 ). The antifungal ability of Streptomyces might be related to antibiosis, nutrient competition, degradative enzyme biosynthesis, nitrous oxide production, and quorum quenching ( Cohen and Mazzola, 2006 ).…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, these compounds identified by GC-MS were different from those synthesized by the above gene clusters ( Table 1 and Supplementary Figure 2 ). This might be due to the diffidence of identification methods and alignment databases ( Wei et al, 2020 ). Thus, we propose that these compounds could altogether contribute to the broad-spectrum antifungal activity of stain JBS5-6 against the tested fungal pathogens.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Newly Isolated Streptomyces sp. JBS5-6 as a Potential Biocontrol Agent to Control Banana Fusarium Wilt: Genome Sequencing and Secondary Metabolite Cluster Profiles

et al. 2020

Self Cite

View full text Add to dashboard Cite

Banana is a key staple food and fruit in countries all over the world. However, the development of the global banana industry is seriously threatened by Fusarium wilt disease, which is caused by Fusarium oxysporum f. sp. cubense (Foc). In particular, Foc tropical race 4 (Foc TR4) could infect more than 80% of global banana and plantain crops. Until now, there were no commercial chemicals or resistant cultivars available to control the disease. Biological control using actinomycetes is considered a promising strategy. In this study, 88 actinomycetes were isolated from a banana orchard without symptoms of Fusarium wilt disease for more than 10 years. An actinobacterial strain labeled as JBS5-6 has exhibited strong antifungal activities against Foc TR4 and other selected 10 phytopathogenic fungi. Based on phenotypic and biochemical traits as well as complete genome analysis, strain JBS5-6 was assigned to Streptomyces violaceusniger. Extracts of the strain inhibited the mycelial growth and spore germination of Foc TR4 by destroying membrane integrity and the ultrastructure of cells. The complete genome of strain JBS5-6 was sequenced and revealed a number of key function gene clusters that contribute to the biosynthesis of active secondary metabolites. Sixteen chemical compounds were further identified by gas chromatography-mass spectrometry (GC-MS). 5-hydroxymethyl-2-furancarboxaldehyde was one of the dominant components in strain JBS5-6 extracts. Moreover, fermentation broth of strain JBS5-6 significantly reduced the disease index of banana seedlings by inhibiting the infection of Foc TR4 in a pot experiment. Hence, strain JBS5-6 is a potential biocontrol agent for the management of disease and the exploitation of biofertilizer.

show abstract

“…Therefore, the latest research trends have focused on developing new screening technologies for efficient dereplication and investigating rare samples from extreme and unexplored environments where microorganisms may have unique characteristics compared with those of known species and thus might produce novel bioactive secondary metabolites ( Suzuki, 2001 ). Extreme and unexplored environments include marine environments ( Yang et al, 2020 ), mountains ( Wei et al, 2020 ), deserts ( Sivakala et al, 2021 ), and rainforests ( Schneider et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Activity and Identification of the Biosynthetic Gene Cluster of X-14952B From Streptomyces sp. 135

Chen

Yan

et al. 2021

Front. Microbiol.

View full text Add to dashboard Cite

The bacterial genus Streptomyces is an important source of antibiotics, and genome mining is a valuable tool to explore the potential of microbial biosynthesis in members of this genus. This study reports an actinomycete strain 135, which was isolated from Qinghai-Tibet Plateau in China and displayed broad antimicrobial activity. The fermentation broth of strain 135 displayed strong antifungal activity (>70%) against Sclerotinia sclerotiorum, Botrytis cinerea, Valsa mali, Phytophthora capsici, Glomerella cingulata, Magnaporthe grisea, Bipolaris maydis, Exserohilum turcicum in vitro, meanwhile possessed significant preventive and curative efficacy against S. sclerotiorum, Gaeumannomyces graminis, and P. capsici on rape leaves (54.04 and 74.18%), wheat (90.66 and 67.99%), and pepper plants (79.33 and 66.67%). X-14952B showed the greatest antifungal activity against S. sclerotiorum and Fusarium graminearum which the 50% inhibition concentration (EC50) were up to 0.049 and 0.04 μg/mL, respectively. Characterization of strain 135 using a polyphasic approach revealed that the strain displayed typical features of the genus Streptomyces. 16S rRNA gene sequencing and phylogenetic analysis demonstrated that the isolate was most closely related to and formed a clade with Streptomyces huasconensis HST28T (98.96% 16S rRNA gene sequence similarity). Average nucleotide identity (ANI) and DNA-DNA hybridization (DDH) values in strain 135 and related type strains were both below the threshold of species determination (91.39 and 56.5%, respectively). OrthoANI values between strain 135 and related type strains are under the cutoff of determining species (<95%). The biosynthetic gene cluster (BGC) designated to X-14952B biosynthesis was identified through genome mining and the possible biosynthesis process was deduced.

show abstract

A Newly Isolated Streptomyces sp. YYS-7 With a Broad-Spectrum Antifungal Activity Improves the Banana Plant Resistance to Fusarium oxysporum f. sp. cubense Tropical Race 4

Cited by 52 publications

References 70 publications

A Novel Antifungal Actinomycete Streptomyces sp. Strain H3-2 Effectively Controls Banana Fusarium Wilt

A Novel Antifungal Actinomycete Streptomyces sp. Strain H3-2 Effectively Controls Banana Fusarium Wilt

Newly Isolated Streptomyces sp. JBS5-6 as a Potential Biocontrol Agent to Control Banana Fusarium Wilt: Genome Sequencing and Secondary Metabolite Cluster Profiles

Antimicrobial Activity and Identification of the Biosynthetic Gene Cluster of X-14952B From Streptomyces sp. 135

Contact Info

Product

Resources

About