A NMR-Based Metabonomics Approach to Determine Protective Effect of a Combination of Multiple Components Derived from Naodesheng on Ischemic Stroke Rats

Luo, Lan; Kang, Jiazhen; He, Qiong; Qi, Yue; Chen, Xingyu; Wang, Shumei; Liang, Shengwang

doi:10.3390/molecules24091831

Cited by 14 publications

(9 citation statements)

References 32 publications

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“…In addition, Nelson et al ( 68 ) used LC/MS to determine 144 metabolites from 367 acute stroke patients, compared cardiac and non-cardiogenic samples, and they found that the tricarboxylic acid metabolite α-ketoglutarate and malate were associated with cardiogenic stroke, which is encouragingly consistent with our findings. Meanwhile, other metabolites we found have also been widely reported by researchers, for example, the changing content of uridine ( 69 ), 3-hydroxybutyric acid ( 70 ), L-cysteine ( 71 ), and asymmetric dimethylarginine ( 72 ).…”

Section: Discussionsupporting

confidence: 67%

Comprehensive metabolomic characterization of atrial fibrillation

Liu

Mei

et al. 2022

Front. Cardiovasc. Med.

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BackgroundUsing human humoral metabolomic profiling, we can discover the diagnostic biomarkers and pathogenesis of disease. The specific characterization of atrial fibrillation (AF) subtypes with metabolomics may facilitate effective and targeted treatment, especially in early stages.ObjectivesBy investigating disturbed metabolic pathways, we could evaluate the diagnostic value of biomarkers based on metabolomics for different types of AF.MethodsA cohort of 363 patients was enrolled and divided into a discovery and validation set. Patients underwent an electrocardiogram (ECG) for suspected AF. Groups were divided as follows: healthy individuals (Control), suspected AF (Sus-AF), first diagnosed AF (Fir-AF), paroxysmal AF (Par-AF), persistent AF (Per-AF), and AF causing a cardiogenic ischemic stroke (Car-AF). Serum metabolomic profiles were determined by gas chromatography–mass spectrometry (GC-MS) and liquid chromatography–quadrupole time-of-flight mass spectrometry (LC-QTOF-MS). Metabolomic variables were analyzed with clinical information to identify relevant diagnostic biomarkers.ResultsThe metabolic disorders were characterized by 16 cross-comparisons. We focused on comparing all of the types of AF (All-AFs) plus Car-AF vs. Control, All-AFs vs. Car-AF, Par-AF vs. Control, and Par-AF vs. Per-AF. Then, 117 and 94 metabolites were identified by GC/MS and LC-QTOF-MS, respectively. The essential altered metabolic pathways during AF progression included D-glutamine and D-glutamate metabolism, glycerophospholipid metabolism, etc. For differential diagnosis, the area under the curve (AUC) of specific metabolomic biomarkers ranged from 0.8237 to 0.9890 during the discovery phase, and the predictive values in the validation cohort were 78.8–90.2%.ConclusionsSerum metabolomics is a powerful way to identify metabolic disturbances. Differences in small–molecule metabolites may serve as biomarkers for AF onset, progression, and differential diagnosis.

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Section: Discussionsupporting

confidence: 67%

Comprehensive metabolomic characterization of atrial fibrillation

Liu

Mei

et al. 2022

Front. Cardiovasc. Med.

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“…Modern scholars of TCM also point out that blood stasis is the principal problem in the process of occurrence, development, and outcome of ischemic stroke (Wang et al, 2014;Zhong et al, 2015;Sun et al, 2016;Chai and Zhang, 2018). Therefore, in the prevention (Chang et al, 2016;Tsai et al, 2016;Song et al, 2019), treatment (Chen et al, 2019;Hsu et al, 2019;Luo et al, 2019), and rehabilitation (Huang et al, 2018;Zhao et al, 2018) of ischemic stroke, conventional treatment combined with drugs that promote blood circulation and remove blood stasis can be an effective intervention.…”

Section: Introductionmentioning

confidence: 99%

Effect of Panax notoginseng Saponins on Focal Cerebral Ischemia-Reperfusion in Rat Models: A Meta-Analysis

et al. 2021

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With the increase of the aging population, the high mortality and disability rates caused by ischemic stroke are some of the major problems facing the world, and they dramatically burden the society. Panax notoginseng (Burk) F. H. Chen, a traditional Chinese medicine, is commonly used for promoting blood circulation and removing blood stasis, and its main bioactive components are Panax notoginseng saponins (PNS). Therefore, we performed a meta-analysis on focal cerebral ischemia-reperfusion animal models established with middle cerebral artery occlusion (MCAO) surgery to evaluate the therapeutic effect of PNS. We systematically searched the reports of PNS in MCAO animal experiments in seven databases. We assessed the study quality using two literature quality evaluation criteria; evaluated the efficacy of PNS treatment based on the outcomes of the neurological deficit score (NDS), cerebral infarct volume (CIV), and biochemical indicators via a random/fixed-effects model; and performed a subgroup analysis utilizing ischemia duration, drug dosage, intervention time, and administration duration. We also compared the efficacy of PNS with positive control drugs or combination treatment. As a result, we selected 14 eligible studies from the 3,581 searched publications based on the predefined exclusion-inclusion criteria. PNS were significantly associated with reduced NDS, reduced CIV, and inhibited release of the inflammatory factors IL-1β and TNF-α in the focal MCAO rat models. The PNS combination therapy outperformed the PNS alone. In addition, ischemia time, drug dosage, intervention time, and administration duration in the rat models all had significant effects on the efficacy of PNS. Although more high-quality studies are needed to further determine the clinical efficacy and guiding parameters of PNS, our results also confirmed that PNS significantly relieves the focal cerebral ischemia-reperfusion in rat models. In the animal trials, it was suggested that an early intervention had significant efficacy with PNS alone or PNS combination treatment at a dosage lower than 25 mg/kg or 100–150 mg/kg for 4 days or longer. These findings further guide the therapeutic strategy for clinical cerebral ischemic stroke.

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“…In addition, NDS also exhibits significant antioxidative activity, as showed by a decrease of LDH and MDA production, increase of SOD generation in plasma, and enhancement of brain levels of leucine, isoleucine, choline, and myo-inositol. 96 NG-R1 is also the main bioactive and circulating compound of XueShuanTong, which has been predicted to show high potentials for drug interactions mediated by organic anion-transporting polypeptide (OATP)1B. 97 Conventional pharmacotherapy has been implicated in the treatment of complex diseases, and the use of herbal medicinal products or botanical dietary supplements is prevalent in China.…”

Section: Clinical Perspectivesmentioning

confidence: 99%

<p>Focus on Notoginsenoside R1 in Metabolism and Prevention Against Human Diseases</p>

Liu

Yang

et al. 2020

DDDT

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Notoginsenoside (NG)-R1 is one of the main bioactive compounds from Panax notoginseng (PN) root, which is well known in the prescription for mediating the microcirculatory hemostasis in human. In this article, we mainly discuss NG-R1 in metabolism and the biological activities, including cardiovascular protection, neuro-protection, anti-diabetes, liver protection, gastrointestinal protection, lung protection, bone metabolism regulation, renal protection, and anti-cancer. The metabolites produced by deglycosylation of NG-R1 exhibit higher permeability and bioavailability. It has been extensively verified that NG-R1 may ameliorate ischemia-reperfusion (IR)-induced injury in cardiovascular and neuronal systems mainly by upregulating the activity of estrogen receptor α-dependent phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT) and nuclear factor erythroid-2-related factor 2 (NRF2) pathways and downregulating nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) pathways. However, no specific targets for NG-R1 have been identified. Expectedly, NG-R1 has been used as a main bioactive compound in many Traditional Chinese Medicines clinically, such as Xuesaitong, Naodesheng, XueShuanTong, ShenMai, and QSYQ. These suggest that NG-R1 exhibits a significant potency in drug development.

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A NMR-Based Metabonomics Approach to Determine Protective Effect of a Combination of Multiple Components Derived from Naodesheng on Ischemic Stroke Rats

Cited by 14 publications

References 32 publications

Comprehensive metabolomic characterization of atrial fibrillation

Comprehensive metabolomic characterization of atrial fibrillation

Effect of Panax notoginseng Saponins on Focal Cerebral Ischemia-Reperfusion in Rat Models: A Meta-Analysis

<p>Focus on Notoginsenoside R1 in Metabolism and Prevention Against Human Diseases</p>

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