A non-oxidative electrochemical approach to online measurements of dopamine release through laccase-catalyzed oxidation and intramolecular cyclization of dopamine

Lin, Yuqing; Zhang, Zipin; Zhao, Lingzhi; Xiang, Wu; Yu, Ping; Su, Lei; Mao, Lanqun

doi:10.1016/j.bios.2009.10.028

Cited by 57 publications

(21 citation statements)

References 60 publications

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“…Then, Lac is used to initialize the sequential intramolecular cyclization reactions of DA, including a deprotonation reaction (Figure 1, reaction 2), an intramolecular cyclization process (reaction 3), and a disproportionation reaction and/or oxidation (reaction 4). The finally formed 5,6-dihydroxyindoline quinone is readily electrochemically reduced at SPE [32]. On the basis of these reaction properties of DA, the non-oxidative electrochemical approach can be proposed for the determination of DA by measuring the cathodic current of 5,6-dihydroxyindoline quinone at a negative potential (−0.158 V).…”

Section: Resultsmentioning

confidence: 99%

“…The results of Figure 3B and C show that symmetrical redox couple of DA at the Lac/SPE and the Lac/MWCNTs/SPE with the potential difference between anodic and cathodic peaks (ΔE p ) are 0.051 V and 0.044 V, characteristic of a two-electron and two-proton quasi-reversible redox process of DA at both SPEs [32]. No cathodic peak at around −0.150 V is found at these SPEs.…”

Section: Resultsmentioning

confidence: 99%

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A disposable biosensor based on immobilization of laccase with silica spheres on the MWCNTs-doped screen-printed electrode

Zhang

et al. 2012

Chemistry Central Journal

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BackgroundBiosensors have attracted increasing attention as reliable analytical instruments in in situ monitoring of public health and environmental pollution. For enzyme-based biosensors, the stabilization of enzymatic activity on the biological recognition element is of great importance. It is generally acknowledged that an effective immobilization technique is a key step to achieve the construction quality of biosensors.ResultsA novel disposable biosensor was constructed by immobilizing laccase (Lac) with silica spheres on the surface of multi-walled carbon nanotubes (MWCNTs)-doped screen-printed electrode (SPE). Then, it was characterized in morphology and electrochemical properties by scanning electron microscopy (SEM) and cyclic voltammetry (CV). The characterization results indicated that a high loading of Lac and a good electrocatalytic activity could be obtained, attributing to the porous structure, large specific area and good biocompatibility of silica spheres and MWCNTs. Furthermore, the electrochemical sensing properties of the constructed biosensor were investigated by choosing dopamine (DA) as the typical model of phenolic compounds. It was shown that the biosensor displays a good linearity in the range from 1.3 to 85.5 μM with a detection limit of 0.42 μM (S/N = 3), and the Michaelis-Menten constant (Kmapp) was calculated to be 3.78 μM.ConclusionThe immobilization of Lac was successfully achieved with silica spheres to construct a disposable biosensor on the MWCNTs-doped SPE (MWCNTs/SPE). This biosensor could determine DA based on a non-oxidative mechanism in a rapid, selective and sensitive way. Besides, the developed biosensor could retain high enzymatic activity and possess good stability without cross-linking reagents. The proposed immobilization approach and the constructed biosensor offer a great potential for the fabrication of the enzyme-based biosensors and the analysis of phenolic compounds.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

A disposable biosensor based on immobilization of laccase with silica spheres on the MWCNTs-doped screen-printed electrode

Zhang

et al. 2012

Chemistry Central Journal

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“…Numerous modified-electrode approaches for the electrocatalytic discrimination of dopamine oxidation from those of ascorbic and uric acids have been suggested [3,4], with hitherto no success in specific detection of dopamine over other catecholamines. Strategies for specific bioelectroanalytical recognition of dopamine, based on the aptamer electrodes [5,6] and laccase microreactors [7] for in vitro detection of dopamine in serum and brain microdialysates, are not yet applicable for in vivo analysis. Tyrosinase electrodes used for analysis of dopamine in the brain showed a good performance, but their general O2-and catechol-dependence may interfere with the assay robustness [8].…”

Section: Introductionmentioning

confidence: 99%

“Negative electrocatalysis”-based specific analysis of dopamine at basal plane HOPG in the presence of structurally related catecholamines

Álvarez‐Martos

Ferapontova

2018

Electrochemistry Communications

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Oxidations of dopamine and structurally related catecholamines were shown to proceed with the essentially different overpotentials at basal plane HOPG electrodes as compared to glassy carbon electrodes, consistent with the concept of "negative electrocatalysis". Electrode reactions of norepinephrine, epinephrine, and catechol slowed down on HOPG to a higher extent than oxidation of dopamine. Larger overpotentials for norepinephrine, epinephrine, and catechol oxidations allowed analytical discrimination and specific 0.05 to 4 µM detection of dopamine in an artificial cerebrospinal fluid in the presence of 0.5 M ascorbic acid and excessive amounts of norepinephrine, epinephrine and catechol. The results offer a new simple and powerful strategy for designing electrodes for specific analysis of dopamine in biological systems.

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“…Poots et al ( 1976 ) reported that commercially there are about 1,00,000 available dyes with over 7 × 10 5 tonnes of dyestuff produced annually. Dyes are usually resistant to fading on exposure to light, water and also to various chemicals due to their complex chemical structure, and most of them are difficult to decolourise due to their synthetic origin (Lin et al 2010a , b ).…”

Section: Introductionmentioning

confidence: 99%

“…Nanostructured enzyme-based biosensor on fullerene and gold nanoparticles was performed evaluating the detection of polyphenols either in buffer solution or in real wine samples (Lanzellotto et al 2014 ). Lin et al ( 2010a , b ) reported a non-oxidative electrochemical approach to online measurements of dopamine release through laccase-catalyzed oxidation and intramolecular cyclization of dopamine. Polyphenol index in wines is determined by laccase coupled multi-walled carbon nano tubes based biosensor.…”

Section: Introductionmentioning

confidence: 99%

Bioprospecting and biotechnological applications of fungal laccase

Upadhyay¹,

Shrivastava

Agrawal³

2016

3 Biotech

188

106

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Laccase belongs to a small group of enzymes called the blue multicopper oxidases, having the potential ability of oxidation. It belongs to enzymes, which have innate properties of reactive radical production, but its utilization in many fields has been ignored because of its unavailability in the commercial field. There are diverse sources of laccase producing organisms like bacteria, fungi and plants. In fungi, laccase is present in Ascomycetes, Deuteromycetes, Basidiomycetes and is particularly abundant in many white-rot fungi that degrade lignin. Laccases can degrade both phenolic and non-phenolic compounds. They also have the ability to detoxify a range of environmental pollutants. Due to their property to detoxify a range of pollutants, they have been used for several purposes in many industries including paper, pulp, textile and petrochemical industries. Some other application of laccase includes in food processing industry, medical and health care. Recently, laccase has found applications in other fields such as in the design of biosensors and nanotechnology. The present review provides an overview of biological functions of laccase, its mechanism of action, laccase mediator system, and various biotechnological applications of laccase obtained from endophytic fungi.

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A non-oxidative electrochemical approach to online measurements of dopamine release through laccase-catalyzed oxidation and intramolecular cyclization of dopamine

Cited by 57 publications

References 60 publications

A disposable biosensor based on immobilization of laccase with silica spheres on the MWCNTs-doped screen-printed electrode

A disposable biosensor based on immobilization of laccase with silica spheres on the MWCNTs-doped screen-printed electrode

“Negative electrocatalysis”-based specific analysis of dopamine at basal plane HOPG in the presence of structurally related catecholamines

Bioprospecting and biotechnological applications of fungal laccase

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