A non-radioactive method for measuring Cu uptake in HepG2 cells

Fosset, Cédric; McGaw, Brian A.; Reid, M.D.; McArdle, Harry J

doi:10.1016/j.jinorgbio.2005.01.005

Cited by 20 publications

(15 citation statements)

References 29 publications

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“…A way of better understanding dysregulation of transmembrane Cu transport is to use Cu isotopes. Indeed, in a preliminary study, Fosset et al 6. used copper enriched in 65 Cu for Cu fluxes tracing in cells.…”

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confidence: 99%

Copper transporters are responsible for copper isotopic fractionation in eukaryotic cells

Cadiou

Pichat

Bondanese

et al. 2017

Sci Rep

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Copper isotopic composition is altered in cancerous compared to healthy tissues. However, the rationale for this difference is yet unknown. As a model of Cu isotopic fractionation, we monitored Cu uptake in Saccharomyces cerevisiae, whose Cu import is similar to human. Wild type cells are enriched in 63Cu relative to 65Cu. Likewise, 63Cu isotope enrichment in cells without high-affinity Cu transporters is of slightly lower magnitude. In cells with compromised Cu reductase activity, however, no isotope fractionation is observed and when Cu is provided solely in reduced form for this strain, copper is enriched in 63Cu like in the case of the wild type. Our results demonstrate that Cu isotope fractionation is generated by membrane importers and that its amplitude is modulated by Cu reduction. Based on ab initio calculations, we propose that the fractionation may be due to Cu binding with sulfur-rich amino acids: methionine and cysteine. In hepatocellular carcinoma (HCC), lower expression of the STEAP3 copper reductase and heavy Cu isotope enrichment have been reported for the tumor mass, relative to the surrounding tissue. Our study suggests that copper isotope fractionation observed in HCC could be due to lower reductase activity in the tumor.

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confidence: 99%

Copper transporters are responsible for copper isotopic fractionation in eukaryotic cells

Cadiou

Pichat

Bondanese

et al. 2017

Sci Rep

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“…We showed that the suppression of neuronal differentiation by BCS was restored by the addition of a low level of Cu(II)Cl 2 . Since it has been reported that a copper(II)-histidine complex was incorporated into cell culture, 20,21) we suggest that copper(II) rapidly binds to small molron-specific genes such as dopamine β-hydroxylase (DBH) and choline acetyltransferase (ChAT) by semi-quantitative RT-PCR analysis. As expected, the expression of these genes was strongly induced by RA treatment with cellular aggregation for 3 days (Fig.…”

Section: Copper Deficiency Suppresses Ra-induced Neuronal Differentiamentioning

confidence: 98%

Copper is Required for Retinoic Acid Receptor-Dependent Transcription and Neuronal Differentiation in P19 Embryonal Carcinoma Cells

Watanabe

Tezuka

2006

JOURNAL OF HEALTH SCIENCE

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“…Fosset et al [52] used this powerful technique as a tool to measure the uptake of Cu with natural isotopes in HepG2 cells, a liver cell line used extensively to study Cu metabolism. And the 63 Cu/ 65 Cu ratio can be measured accurately.…”

Section: Analysis Of Elements Related To Human Healthmentioning

confidence: 99%

The application of inductively coupled plasma mass spectrometry in pharmaceutical and biomedical analysis

Huang

Zhang

et al. 2006

Journal of Pharmaceutical and Biomedical Analysis

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A non-radioactive method for measuring Cu uptake in HepG2 cells

Cited by 20 publications

References 29 publications

Copper transporters are responsible for copper isotopic fractionation in eukaryotic cells

Copper transporters are responsible for copper isotopic fractionation in eukaryotic cells

Copper is Required for Retinoic Acid Receptor-Dependent Transcription and Neuronal Differentiation in P19 Embryonal Carcinoma Cells

The application of inductively coupled plasma mass spectrometry in pharmaceutical and biomedical analysis

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