A note on the isolation of <i>Staphylococcus</i> aureus from raw minced meat

Isigidi, B.K.; Devriese, Luc; Hoof, Jan Van

doi:10.1111/j.1365-2672.1985.tb03339.x

Cited by 9 publications

(3 citation statements)

References 10 publications

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“…. The results obtained with pure cultures on BPD (Table 1) illustrate the inhibitory effects of this highly selective medium on stressed cells, as already noted by Becker et al (1983), Harvey & Gilmour (1985) and Isigidi et al (1985). The results obtained with BPb/BPD were similar to those with BP.…”

Section: Discussionsupporting

confidence: 83%

A highly selective two‐stage isolation method for the enumeration of Staphylococcus aureus in foods

Isigidi¹,

Devriese²,

Croegaert³

et al. 1989

Journal of Applied Bacteriology

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A plate method for enumerating Staphylococcus aureus is described which combines a 1‐h recovery period for stressed cells on a relatively non‐selective Baird‐Parker agar base followed by a 24‐h growth phase in a highly selective, supplemented Baird‐Parker medium added as an overlay. Tests with pure cultures showed satisfactory recovery of stressed Staph. aureus and other bacteria. Similar results were obtained with the conventional Baird‐Parker procedure and with the two‐stage isolation method for shrimps and poultry neck skins, but for raw minced meat, recoveries were higher with the combined method than with the conventional medium. All colonies visible after 24 h on the two‐stage medium can be counted as Staph. aureus, whereas longer incubation times and confirmatory tests are necessary to differentiate it from other organisms on conventional Baird‐Parker medium.

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Section: Discussionsupporting

confidence: 83%

A highly selective two‐stage isolation method for the enumeration of Staphylococcus aureus in foods

Isigidi¹,

Devriese²,

Croegaert³

et al. 1989

Journal of Applied Bacteriology

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“…The MSSA ATCC29213 strain which was one of common sensitive reference strains [21] was provided by the Department of Laboratory Medicine (West China Hospital, Sichuan University, Chengdu, China) while the clinical MRSA strains were isolated from clinical specimens described above. Pure growth of single clones was achieved on conventional Baird-Parker (BP) agar [22], and the colonies were sequenced for strain identification (Additional file 1). S. aureus strains were cultured [23] (Additional file 1).…”

Section: Methodsmentioning

confidence: 99%

Staphylococcus aureus biofilm organization modulated by YycFG two-component regulatory pathway

Huang

Zhang

et al. 2019

J Orthop Surg Res

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BackgroundStaphylococcus aureus (S. aureus) infection accounts for more than 50% of the osteomyelitis cases. Currently, methicillin-resistant S. aureus (MRSA) strains present an urgent medical problem. The YycFG two-component regulatory system (TCS) can allow bacteria to rapidly adapt to physical, chemical, and biological stresses. To define the role of YycFG in modulation virulence of S. aureus in osteomyelitis, we isolated clinical MRSA strains and compared these with ATCC29213 methicillin-sensitive S. aureus (MSSA).MethodsIn the present study, 13 MRSA strains from chronic osteomyelitis tissues were isolated. The in-depth sequencing of 16S rRNA amplicons of the samples was conducted. Bacterial growth was monitored, and biofilm biomass was determined by crystal violet microtiter assay. Furthermore, quantitative RT-PCR analysis was adopted to identify the expression of yycF/G/H and icaA/D in MRSA and MSSA strains. Analysis of variance with one-way ANOVA was used for statistical analysis.ResultsThe in-depth sequencing of 16S rRNA amplicons of the clinical samples indicated a polymicrobial infection, with the phylum Firmicutes made up 13% of the microbial population. The MRSA strains showed an accelerated growth rate compared to the MSSA strains. Of note, MRSA biofilms showed an accumulation of an intercellular polysaccharides matrix and enhanced biomass upon microscopic examination. Furthermore, MRSA strains had a higher expression of the yycF/G/H and icaA/D genes and adhesion force.ConclusionsThese data suggested the roles of intercellular polysaccharide in S. aureus pathogenesis, indicating a possible association between YycFG pathways and MRSA strain virulence.Electronic supplementary materialThe online version of this article (10.1186/s13018-018-1055-z) contains supplementary material, which is available to authorized users.

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“…As amostras de carnes cozidas apresentaram-se fora do padrão legal que é de 10 2 UFC/g, e das carnes cruas uma não atendeu o padrão, estando acima de 10 3 UFC/g de S. aureus [8]. Resultados superiores foram obtidos por ISIGIDI, DEVRIESE, VAN HOOF [30] em Gent-Bélgica, quando estudaram 52 amostras de carne moída crua e por FLORENTINO et al [15], que analisaram carne moída comercializada em João Pessoa-PB. Dados semelhantes aos encontrados neste trabalho foram obtidos por FUZIHARA & FRANCO [17] em carne suína, na cidade de Santo André-SP.…”

Section: -Resultados E Discussãounclassified

Staphylococcus enterotoxigênicos em alimentos in natura e processados no estado de Pernambuco, Brasil

Neto¹,

Silva²,

Stamford³

2002

Ciênc. Tecnol. Aliment.

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A note on the isolation of Staphylococcus aureus from raw minced meat

Cited by 9 publications

References 10 publications

A highly selective two‐stage isolation method for the enumeration of Staphylococcus aureus in foods

A highly selective two‐stage isolation method for the enumeration of Staphylococcus aureus in foods

Staphylococcus aureus biofilm organization modulated by YycFG two-component regulatory pathway

Staphylococcus enterotoxigênicos em alimentos in natura e processados no estado de Pernambuco, Brasil

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