A Novel Activated Biochar-Based Immunosensor for Rapid Detection of E. coli O157:H7

Sobhan, Abdus; Jia, Fei; Kelso, Lisa Cooney; Biswas, Sonatan Kumar; Muthukumarappan, Kasiviswanathan; Cao, Changyong; Wei, Lin; Li, Yanbin

doi:10.3390/bios12100908

Cited by 13 publications

(7 citation statements)

References 39 publications

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“…An example of an electrochemical biosensor can be found in the work of Sobhan et al (2022) [ 5 ]. In their research, they developed an activated biochar-based immunosensor for the detection of Escherichia coli ( E. coli ) cells in a pure culture.…”

Section: Introductionmentioning

confidence: 99%

Development of an Immunocapture-Based Polymeric Optical Fiber Sensor for Bacterial Detection in Water

Lopes,

Pinto,

Vargas

et al. 2024

Polymers

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Conventional methods for pathogen detection in water rely on time-consuming enrichment steps followed by biochemical identification strategies, which require assay times ranging from 24 hours to a week. However, in recent years, significant efforts have been made to develop biosensing technologies enabling rapid and close-to-real-time detection of waterborne pathogens. In previous studies, we developed a plastic optical fiber (POF) immunosensor using an optoelectronic configuration consisting of a U-Shape probe connected to an LED and a photodetector. Bacterial detection was evaluated with the immunosensor immersed in a bacterial suspension in water with a known concentration. Here, we report on the sensitivity of a new optoelectronic configuration consisting of two POF U-shaped probes, one as the reference and the other as the immunosensor, for the detection of Escherichia coli. In addition, another methos of detection was tested where the sensors were calibrated in the air, before being immersed in a bacterial suspension and then read in the air. This modification improved sensor sensitivity and resulted in a faster detection time. After the immunocapture, the sensors were DAPI-stained and submitted to confocal microscopy. The histograms obtained confirmed that the responses of the immunosensors were due to the bacteria. This new sensor detected the presence of E. coli at 104 CFU/mL in less than 20 min. Currently, sub-20 min is faster than previous studies using fiber-optic based biosensors. We report on an inexpensive and faster detection technology when compared with conventional methods.

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Section: Introductionmentioning

confidence: 99%

Development of an Immunocapture-Based Polymeric Optical Fiber Sensor for Bacterial Detection in Water

Lopes,

Pinto,

Vargas

et al. 2024

Polymers

View full text Add to dashboard Cite

show abstract

“…An example of an electrochemical biosensor can be found in the work of Sobhan et al (2022) [5]. In their research they developed an activated biochar-based immunosensor for the detection of Escherichia coli (E. coli) cells in pure culture.…”

Section: Introductionmentioning

confidence: 99%

Development of an Immunocapture-Based Polymeric Optical Fiber Sensor for Bacteria Detection

Lopes,

Pinto,

Vargas

et al. 2024

Preprint

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Conventional methods for pathogen detection in water rely on time-consuming enrichment steps followed by biochemical identification strategies, which require assay times ranging from 24 hours to up to a week. However, in recent years, significant efforts have been made towards the development of biosensing technologies enabling rapid and close-to-real-time detection of waterborne pathogens. In previous studies, we developed a plastic optical fiber (POF) immunosensor using an optoelectronic configuration consisting of a U-Shape probe connected to an LED and a photodetector. Bacterial detection was evaluated with the immunosensor immersed in a bacterial suspension in water with a known concentration. Here, we report on the sensitivity of a new optoelectronic configuration consisting of two POF U-Shape probes, one as the reference and the other as the immunosensor, for the detection of Escherichia coli. In addition, another way of detection was tested where the sensors were calibrated in the air, before immersed in bacterial suspension and then read in the air, making the immunosensor more sensitive and with a faster detection time. This new sensor detected the presence of E. coli at 104 CFU/mL in less than 10 minutes. Currently sub 10 minutes is faster than previous studies using fiber-optic based biosensors. It is also a much simpler and quicker methodology when compared with conventional laboratory technology.

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“…Although these methods have high accuracy and speed, the high cost and need for specialized knowledge and complex equipment are the disadvantages of these methods [13]. Several types of biosensors such as aptasensor [14], immunosensor [15], carbohydrate biosensor [16], and enzymatic biosensor [17] have been developed to identify pathogens including E. coli bacteria using different techniques such as electrochemical [18], colorimetric [19], optical [20], and quartz crystal microbalance (QCM) methods [21].…”

Section: Introductionmentioning

confidence: 99%

First Label-Free Carbohydrate-Based Electrochemical Sensor To Detect Escherichia coli Pathogenic Bacteria Using Affinity Between Adhesion FimH and Mannose on the Glassy Carbon Electrode

Zadeha¹,

Kashanian²,

Nazari³

2023

Preprint

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Controlling water and food contamination by pathogenic organisms requires quick, simple, and low-cost methods. using affinity between mannose and type I fimbriae in the cell wall of Escherichia coli bacteria as evaluation elements compared to the conventional plate counting technique enables a reliable sensing platform for the detection of bacteria. In this study, a simple new sensor based on electrochemical impedance spectroscopy (EIS) for rapid and sensitive detection of Escherichia coli was developed. Biorecognition layer of the sensor was formed by covalent attachment of p-carboxyphenylamino mannose (PCAM) to gold nanoparticles (AuNPs) electrodeposited on the surface of glassy carbon electrode (GCE). The resultant structure of PCAM was characterized and confirmed using FTIR. The developed biosensor demonstrated a linear response with a logarithm of bacterial concentration (R2 = 0.998) in the range of 1.3 × 10 1~ 1.3 × 106 CFU.mL−1with the limit of detection of 2 CFU.mL−1 within 60 min. The sensor did not generate any significant signals with two non-target strains, demonstrating high selectivity of the developed biorecognition chemistry. Selectivity of the sensor and its applicability to analysis of the real samples was investigated in samples of tap water. Overall, the developed sensor has shown to be promising for the detection of E. coli pathogen in water due to its high sensitivity, short detection time, low cost, high specificity, and user-friendliness.

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A Novel Activated Biochar-Based Immunosensor for Rapid Detection of E. coli O157:H7

Cited by 13 publications

References 39 publications

Development of an Immunocapture-Based Polymeric Optical Fiber Sensor for Bacterial Detection in Water

Development of an Immunocapture-Based Polymeric Optical Fiber Sensor for Bacterial Detection in Water

Development of an Immunocapture-Based Polymeric Optical Fiber Sensor for Bacteria Detection

First Label-Free Carbohydrate-Based Electrochemical Sensor To Detect Escherichia coli Pathogenic Bacteria Using Affinity Between Adhesion FimH and Mannose on the Glassy Carbon Electrode

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