A novel adjuvant Ling Zhi-8 for cancer DNA vaccines

Chu, Chih‐Liang; Chen, Dz-Chi; Lin, Chi-Chen

doi:10.4161/hv.7.11.17753

Cited by 10 publications

(8 citation statements)

References 33 publications

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“…The results showed that rLZ-8 increased the antitumor activity of the vaccine, promoting its Th1 response via the activation of DCs, evidencing the adjuvant potential of rLZ-8 [ 130 ]. Based on this evidence, the authors anticipate that further clinical trials administering anticancer DNA vaccines along with rLZ-8 as an adjuvant agent are possible [ 131 ].…”

Section: In Vivo Studiesmentioning

confidence: 99%

Ganoderma spp.: A Promising Adjuvant Treatment for Breast Cancer

et al. 2017

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For the past several decades, cancer patients in the U.S. have chosen the use of natural products as an alternative or complimentary medicine approach to treat or improve their quality of life via reduction or prevention of the side effects during or after cancer treatment. The genus Ganoderma includes about 80 species of mushrooms, of which several have been used for centuries in traditional Asian medicine for their medicinal properties, including anticancer and immunoregulatory effects. Numerous bioactive compounds seem to be responsible for their healing effects. Among the approximately 400 compounds produced by Ganoderma spp., triterpenes, peptidoglycans and polysaccharides are the major physiologically-active constituents. Ganoderma anticancer effects are attributed to its efficacy in reducing cancer cell survival and growth, as well as by its chemosensitizing role. In vitro and in vivo studies have been conducted in various cancer cells and animal models; however, in this review, we focus on Ganoderma's efficacy on breast cancers. Evidence shows that some species of Ganoderma have great potential as a natural therapeutic for breast cancer. Nevertheless, further studies are needed to investigate their potential in the clinical setting and to translate our basic scientific findings into therapeutic interventions for cancer patients.

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Section: In Vivo Studiesmentioning

confidence: 99%

Ganoderma spp.: A Promising Adjuvant Treatment for Breast Cancer

et al. 2017

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“…In previous studies, we established that the naked HER-2/neu DNA vaccine delivered via gene gun can be used for therapy in p185neu-expressing MBT-2 tumors in C3H/HeN mice [22]. Therefore, we tested the possibility of using AC hmwPSs as adjuvants in this mouse DNA vaccine model.…”

Section: Resultsmentioning

confidence: 99%

The Adjuvant Effects of High-Molecule-Weight Polysaccharides Purified from Antrodia cinnamomea on Dendritic Cell Function and DNA Vaccines

Lin

Pan

et al. 2015

PLoS ONE

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The biological activity of the edible basidiomycete Antrodia cinnamomea (AC) has been studied extensively. Many effects, such as anti-cancer, anti-inflammatory, and antioxidant activities, have been reported from either crude extracts or compounds isolated from AC. However, research addressing the function of AC in enhancing immunity is rare. The aim of the present study is to investigate the active components and the mechanism involved in the immunostimulatory effect of AC. We found that polysaccharides (PS) in the water extract of AC played a major role in dendritic cell (DC) activation, which is a critical leukocyte in initiating immune responses. We further size purified and identified that the high-molecular weight PS fraction (greater than 100 kDa) exhibited the activating effect. The AC high-molecular weight PSs (AC hmwPSs) promoted pro-inflammatory cytokine production by DCs and the maturation of DCs. In addition, DC-induced antigen-specific T cell activation and Th1 differentiation were increased by AC hmwPSs. In studying the molecular mechanism, we confirmed the activation of the MAPK and NF-κB pathways in DCs after AC hmwPSs treatment. Furthermore, we demonstrated that TLR2 and TLR4 are required for the stimulatory activity of AC hmwPSs on DCs. In a mouse tumor model, we demonstrated that AC hmwPSs enhanced the anti-tumor efficacy of the HER-2/neu DNA vaccine by facilitating specific Th1 responses. Thus, we conclude that hmwPSs are the major components of AC that stimulate DCs via the TLR2/TLR4 and NF-κB/MAPK signaling pathways. The AC hmwPSs have potential to be applied as adjuvants.

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“…Mouse DCs were generated from bone marrow (BM) as previously described [ 14 , 28 ]. Briefly, BM cells were isolated from the femur and tibia of the mice.…”

Section: Methodsmentioning

confidence: 99%

“…Based on the sequence and structure, FIP-vvo belongs to the Fve-type FIPs. FIPs have various activities that affect immunity by regulating the cell-mediated or/and humoral immune system, resulting in anti-allergic activity [ 6 , 7 , 8 , 9 ], anti-inflammatory functions [ 10 , 11 , 12 , 13 ], and anti-tumor effects [ 14 , 15 , 16 , 17 ]. However, how FIP-vvo regulates the immune responses has not yet been studied.…”

Section: Introductionmentioning

confidence: 99%

The Enhancing Effect of Fungal Immunomodulatory Protein-Volvariella Volvacea (FIP-vvo) on Maturation and Function of Mouse Dendritic Cells

Lee

et al. 2021

Life

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Volvariella volvacea, also known as straw mushroom, is a common edible mushroom in Chinese cuisine. It contains many nutrients for human health. A fungal immunomodulatory protein (FIP) has been isolated from V. volvacea and named FIP-vvo. Although the regulatory effects of many FIPs on immunity have been identified, the impact of FIP-vvo in modulating dendritic cells (DCs), which play a key role to connect the innate and the adaptive immunity, is not known. In this study, we aim to study the effect of FIP-vvo on the DC maturation and function. We found that FIP-vvo slightly increased the generation of CD11c+ bone marrow-derived DC (BMDC). In addition, the surface expression of MHCII was promoted in BMDCs after the treatment of FIP-vvo, suggesting that FIP-vvo induces DC maturation. Furthermore, FIP-vvo enhanced the ability of BMDCs to activate antigen-specific T cell responses in vitro. In the in vivo study, the FIP-vvo treatment facilitated T cell response in lymph nodes. Therefore, for the first time, our data demonstrated that FIP-vvo promoted DC maturation and function and suggested that FIP-vvo could have benefits for human health by enhancing immunity.

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A novel adjuvant Ling Zhi-8 for cancer DNA vaccines

Cited by 10 publications

References 33 publications

Ganoderma spp.: A Promising Adjuvant Treatment for Breast Cancer

Ganoderma spp.: A Promising Adjuvant Treatment for Breast Cancer

The Adjuvant Effects of High-Molecule-Weight Polysaccharides Purified from Antrodia cinnamomea on Dendritic Cell Function and DNA Vaccines

The Enhancing Effect of Fungal Immunomodulatory Protein-Volvariella Volvacea (FIP-vvo) on Maturation and Function of Mouse Dendritic Cells

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