A novel AgNP/DNA/TPdye conjugate-based two-photon nanoprobe for GSH imaging in cell apoptosis of cancer tissue

Tang, Qinglin; Wang, Ningning; Zhou, Fulin; Deng, Ting; Zhang, Songbai; Li, Jishan; Yang, Ronghua; Zhong, Wenwan; Tan, Weihong

doi:10.1039/c5cc06471f

Cited by 28 publications

(19 citation statements)

References 30 publications

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“…It should be noticed that the other thiol-containing amino acids such as Cys and Hcy can also cause a slightly decreased for absorbance signal. However, the cellular levels of Cys and Hcy are extremely low, whose levels are thousand times lower than that of GSH, 34 days, and the RSD is 2.5 % calculate by the absorbance at 651 nm (n=10). These results showed good stability of our assay.…”

Section: Optimization Of Sensing Conditionsmentioning

confidence: 99%

Feasibility Study on Facile and One-step Colorimetric Determination of Glutathione by Exploiting Oxidase-like Activity of Fe3O4-MnO2 Nanocomposites

et al. 2021

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A facile and one-step colorimetric assay is described for the determination of glutathione (GSH). It is based on the use of manganese dioxide-decorated magnetic (Fe3O4@MnO2) nanocomposite that were prepared by in-situ redox reaction. They exhibit oxidase-mimicking activity and can catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) without H2O2 to form a blue colored product (oxTMB) with an absorption maximum at 651 nm. Once GSH is introduced, the component of MnO2 can ben rapidly reduced to Mn 2+ ions, which leads to inhibit the formation of oxTMB. Based on these findings, a one-step colorimetric assay was developed for the detection GSH in the range of 0.2 to 25 μM with a low detection limit of 0.2 μM without using any procedures of separation and washing. Importantly, the proposed approach is also used to accurately evaluate the intracellular GSH levels. In our perception, the assay is rapid, sensitive and specific.

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Section: Optimization Of Sensing Conditionsmentioning

confidence: 99%

Feasibility Study on Facile and One-step Colorimetric Determination of Glutathione by Exploiting Oxidase-like Activity of Fe3O4-MnO2 Nanocomposites

et al. 2021

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“…With the continuous development of nanomaterial preparation technology, more and more emerging nanomaterials, combining with multiphoton imaging, have been applied in the field of biomedical photonics. With the unique properties of nanoprobes, multiphoton imaging can not only detect tumor cells [82] and apoptotic cells, [83,84] but also present the process of endocytosis of nanoprobes in detail. [92,93,96] Yu et al prepared ZnS:Mn by a simple Lewis acid-base reaction method, which exhibited good three-photon excitation performance.…”

Section: Disease Diagnosis Imagingmentioning

confidence: 99%

“…[ 82 ] Tang et al observed 3D images of fresh cervix tumor tissue from 0 to 300 µm using the DNA/AgNP/TPdye nanoprobe. [ 83 ] The fluorescence signals at a depth of 300 µm were also detected in the liver tissue sections of mice through the AuNP/peptide‐TPdye nanoconjugate. [ 84 ] Zong et al prepared DCzPDI with typical aggregation‐induced emission characteristics.…”

Section: Low‐dimensional Nanomaterials For Imagingmentioning

confidence: 99%

“…[ 90 ] They exhibited superior performance in living cells, including excellent two‐photon‐induced fluorescence characteristics, convenient preparation pathways, specific response to proteins, a broader linear range, and high stability. The detections of glutathione, caspase‐3, and H 2 S in HeLa cells were performed using various nanoprobe DNA/AgNP/Tpdye, [ 83 ] AuNP/peptide‐Tpdye, [ 84 ] and TPSNP, [ 91 ] respectively. Wang et al further reported TpASH‐NPHS, which is modified by covalent organic frameworks to have the ability to filter out coexisting interfering components ( Figure a).…”

Section: Low‐dimensional Nanomaterials For Imagingmentioning

confidence: 99%

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Emerging Low‐Dimensional Nanoagents for Bio‐Microimaging

Lin

Yang

Zhang

et al. 2020

Adv Funct Materials

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Nanomaterials with unique physical and biological properties have attracted increased attention for bio-microimaging applications. Among them, lowdimensional nanomaterials have quantum confinement effect in zero, one, or two dimensions, which have high specific surface area for cargo (such as dye) loading, anisotropic optical properties for polarization, and enhanced infrared absorption for efficient photoluminescence. In this review, recent efforts on bio-microimaging of low-dimensional nanomaterials, including confocal imaging, multiphoton imaging, Raman imaging, and super-resolution imaging, will be highlighted. Bio-microimaging principle, respective advantages and bottlenecks, and applications of low-dimensional materials in various imaging technologies will be discussed. Furthermore, the review will shed light on the common synthetic methods of low-dimensional nanomaterials. Finally, the classification and comparison of various nanomaterials, including carbon, black phosphorus, gold, metal composites, and 2D graphene analogues will also be elaborated.

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“…As shown in Scheme , a carbazole derivative, ethyl-4-[3,6-bis(1-methyl-4-vinylpyridium iodine)-9 H -carbazol-9-yl)] butanoate (EBMVC-B), was synthesized, and could rapidly intercalate into DNA grooves with bright fluorescence, due to its large flat aromatic structure and cationic charges (Figure S1). We found that the fluorescence of the fluorophore/DNA could be high-efficiently quenched by the in situ formed CuNPs on DNA scaffold; attractively, when the quenched EBMVCB/DNA-CuNPs were introduced into a CN – -contained system, CuNPs could be etched by CN – based on an Elsner-like reaction, resulting in fluorescence recovery. The lighting of fluorescence could be achieved in 2 s, and thus, a nanolamp was rationally constructed for instantaneous monitoring of CN – .…”

mentioning

confidence: 95%

A Reversible Nanolamp for Instantaneous Monitoring of Cyanide Based on an Elsner-Like Reaction

et al. 2016

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It is well-known that cyanide ion (CN) is a hypertoxic anion, which can cause adverse effects in both the environment and living beings; thus, it is highly desirable to develop strategies for detecting CN, especially in water and food. However, due to the short half-life of free cyanide, long analysis time and/or interference from other competitive ions are general challenges for accurate monitoring of CN. In this work, through the investigation on the sequence-dependent optical interaction of DNA-CuNPs with the fluorophore (e.g., EBMVC-B), we found, for the first time, that DNA-CuNPs were an ideal alternative as fluorescence quencher in constructing a sensor which could be illuminated by CN based on an Elsner-like reaction and that the signal switching was dependent on poly(AT/TA) dsDNA sequence. By virtue of CuNPs' small size and its high chemical reactivity with cyanide, the lighting of fluorescence was ultrarapid and similar to the hairtrigger "turn-on" of a lamp, which is significant for accurately monitoring a target of short half-life (e.g., cyanide). Attributed to the unique Elsner-like reaction between CN and the Cu atoms, high selectivity was achieved for CN monitoring by the nanolamp, with practical applications in real water and food samples. In addition, because of the highly efficient in situ formation of DNA-CuNPs and the approximative stoichiometry between CN and Cu in the fluorescence switching, the nanolamp could be reversibly turned on and off through the alternate regulation of CN and Cu, displaying potential for developing reusable nanosensors and constructing optical molecular logic circuits.

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A novel AgNP/DNA/TPdye conjugate-based two-photon nanoprobe for GSH imaging in cell apoptosis of cancer tissue

Abstract: In the present study, a novel two-photon nanoprobe has been developed and successfully applied in glutathione (GSH) imaging in cell apoptosis of cancer tissue.

Cited by 28 publications

References 30 publications

Feasibility Study on Facile and One-step Colorimetric Determination of Glutathione by Exploiting Oxidase-like Activity of Fe3O4-MnO2 Nanocomposites

Feasibility Study on Facile and One-step Colorimetric Determination of Glutathione by Exploiting Oxidase-like Activity of Fe3O4-MnO2 Nanocomposites

Emerging Low‐Dimensional Nanoagents for Bio‐Microimaging

A Reversible Nanolamp for Instantaneous Monitoring of Cyanide Based on an Elsner-Like Reaction

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