Feasibility Study on Facile and One-step Colorimetric Determination of Glutathione by Exploiting Oxidase-like Activity of Fe3O4-MnO2 Nanocomposites

Zhang, Huiwen; Yao, Shuo; Zhao, Chao; Zhao, Wei; Li, Juan; Wang, Juan

doi:10.2116/analsci.20p353

Cited by 3 publications

(2 citation statements)

References 35 publications

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“…The oxidation of glutathione (GSH) by CD-PEI was validated by 5.5′-dithiobis (2-nitrobenzoic acid) (DTNB). 30 In a standard Oxidase-like activity assay, 100μg CD-PEI was added into a 1.0 mL buffer solution (pH 4.5) and mixed with aliquots of 10μL of DNTB and different concentrations of GSH. The mixtures were detected in the time interval of 10 min in the absorbance of 422 nm.…”

Section: Methodsmentioning

confidence: 99%

Carbon Dots-Based Nanozyme for Drug-Resistant Lung Cancer Therapy by Encapsulated Doxorubicin/siRNA Cocktail

Yu¹,

Tang²,

Cai³

et al. 2023

IJN

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Background Nanomaterials exhibited intrinsic enzyme-like properties due to the unique properties compared with natural enzyme. Carbon dots (CDs) are an important kind of quantum-sized nanomaterials, which have enormous application potential in bio-imaging, drug carrier, and nanosystems. Carbon dots possess intrinsic enzyme-like properties, such as glutathione (GSH) oxidase or peroxidase activities. Methods A co-delivery nanosystem that could carry siRNA and doxorubucin (DOX) simultaneously has been studied in this work. The co-delivery based on carbon dots was surface-modified with poly-ethylenimine (PEI) and loaded the siMRP1 with chemotherapeutics on the surface with pH-triggered drug release. The CD-PEI was synthesized by one-step microwave assisted method; the PEI was raw materials and passivator during the reaction process that makes CDs exhibit excellent optical property. Results The CD-PEI was capable of loading and delivering siMRP1 and DOX to tumors and releasing them synchronously in cells in an acid-triggered manner. The particles exhibited GSH oxidase-like catalytic property, oxidizing GSH to oxidized glutathione with concomitant increase of reactive oxygen species (ROS). We found that silencing of MRP1 by co-delivery system antagonized chemoresistance by increasing DOX accumulation and significantly enhancing the inhibitory effect of cell viability induced by CD-PEI-DOX. The co-delivery system dramatically inhibited tumor growth in xenograft model, and CDs counteracted MRP1 function by siRNA-mediated knockdown of MRP1. Conclusion Taken together, we uncover the potential role of CDs with a combination of siRNA and chemotherapeutics in overcoming chemoresistance of lung cancer by suppressing MRP1 and oxidation of GSH. Our findings imply its potential of antagonizing chemoresistance to enhance therapeutic efficiency of doxorubicin in clinical practices of lung cancer treatment.

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Section: Methodsmentioning

confidence: 99%

Carbon Dots-Based Nanozyme for Drug-Resistant Lung Cancer Therapy by Encapsulated Doxorubicin/siRNA Cocktail

Yu¹,

Tang²,

Cai³

et al. 2023

IJN

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“…These crystal faces are identified as (111), ( 220), (311), (400), ( 422), ( 511), ( 440) and (533) [7]. Besides, the peaks observed at 2θ = 12.8° and 37.6° correspond to the crystal plane reflections of ( 110) and (121), respectively, which can be associated with MnO2 according to the standard card (JCPDS 72-1982) [8]. Contrasting the XRD patterns, no significant deviations are discernible, implying that the MnO2 coating does not induce alterations in the lattice structure of the material.…”

Section: Analysis Of Cufe 2 O 4 /N-gqds@mnomentioning

confidence: 99%

Core-shell rambutan-like nanoenzyme with oxidase-like activity for colorimetric detection of phenol

2024

mpcr

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It is crucial to cultivate a a novel, convenient, and quick colorimetric approach to monitor phenol owing to its huge damage to people, biomass, and the environment. In this study, a rambutan-like nanocomposite CuFe2O4/N-GQDs@MnO2 with the magnetic CuFe2O4 as the central core and MnO2 as the outer shell was synthesized and highlighted. The introduction of nitrogen doped graphene quantum dots (N-GQDs) could not only alter the morphology of nanocomposite, but also link two different metallic oxides tightly. It is demonstrated that the materials prepared have good oxidase-like activity and can catalyze the coupling reaction of 4-aminoantipyrine (4-AAP) and phenol to yield a pink product in the absence of H2O2. Drawing upon this occurrence, a simple, low-cost, and highly selective colorimetric detection platform for phenol was developed. The instrument exhibits a linear response between 5μM and 200μM, with a sensitivity threshold of 0.18μM. In addition, the synthesized materials have good recyclability for the magnetism, indicating that CuFe2O4/N-GQDs@MnO2 nanocomposites have great potential applications in the detection of phenol.

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A swellable hydrogel microneedle based on cerium-metal organic frame composite nanozyme for detection of biomarkers

Zhao,

Lv,

Ling

et al. 2024

International Journal of Biological Macromolecules

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Feasibility Study on Facile and One-step Colorimetric Determination of Glutathione by Exploiting Oxidase-like Activity of Fe3O4-MnO2 Nanocomposites

Cited by 3 publications

References 35 publications

Carbon Dots-Based Nanozyme for Drug-Resistant Lung Cancer Therapy by Encapsulated Doxorubicin/siRNA Cocktail

Carbon Dots-Based Nanozyme for Drug-Resistant Lung Cancer Therapy by Encapsulated Doxorubicin/siRNA Cocktail

Core-shell rambutan-like nanoenzyme with oxidase-like activity for colorimetric detection of phenol

A swellable hydrogel microneedle based on cerium-metal organic frame composite nanozyme for detection of biomarkers

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