A Novel Alphabaculovirus from the Soybean Looper, Chrysodeixis includens, that Produces Tetrahedral Occlusion Bodies and Encodes Two Copies of he65

Abstract

Isolates of the alphabaculovirus species, Chrysodeixis includens nucleopolyhedrovirus, have been identified that produce polyhedral occlusion bodies and infect larvae of the soybean looper, Chrysodeixis includens. In this study, we report the discovery and characterization of a novel C. includens-infecting alphabaculovirus, Chrysodeixis includens nucleopolyhedrovirus #1 (ChinNPV#1), that produces tetrahedral occlusion bodies. In bioassays against C. includens larvae, ChinNPV #1 exhibited a degree of pathogenicity that was similar to that of other ChinNPV isolates, but killed larvae more slowly. The host range of ChinNPV#1 was found to be very narrow, with no indication of infection occurring in larvae of Trichoplusia ni and six other noctuid species. The ChinNPV#1 genome sequence was determined to be 130,540 bp, with 126 open reading frames (ORFs) annotated but containing no homologous repeat (hr) regions. Phylogenetic analysis placed ChinNPV#1 in a clade with other Group II alphabaculoviruses from hosts of lepidopteran subfamily Plusiinae, including Chrysodeixis chalcites nucleopolyhedrovirus and Trichoplusia ni single nucleopolyhedrovirus. A unique feature of the ChinNPV#1 genome was the presence of two full-length copies of the he65 ORF. The results indicate that ChinNPV#1 is related to, but distinct from, other ChinNPV isolates.

Complete Genome Sequences of Seven New Chrysodeixis includens Nucleopolyhedrovirus Isolates from Minas Gerais and Mato Grosso States in Brazil

“…A total of 140 ORFs were annotated in each genome, including all baculovirus core genes, but like other previously sequenced ChinNPV genomes (1, 9; NCBI taxonomic identifier 1207438), no typical baculovirus homologous repeat (hr) regions were found in any of the isolates. No major differences in gene content and order were found compared to all known ChinNPV genomes, other than the ChinNPV#1 isolate, which was already identified as distinct in a previous study (9). The genomes of the seven isolates analyzed here exhibited two copies of the p26 gene, two bro (baculovirus repeated ORF) genes (bro-a and bro-b), one copy of DNA photolyase, and a lack of a lef-12 homolog.…”

Influence of soil management for soybean production under microbial diversity in amazon soils

“…The most relevant viruses identified on cultivated areas were species of Pseudoplusia includens, which are pathogens of the insects Chrysodeixis includen, these are parasites of the soybean crop (Alexandre et al, 2010;Craveiro et al, 2015;Harrison et al, 2019) the presence of these viruses can be considered as biopesticides and/or pest controllers in crops. Pandoraviruses, which were identified in the four sites, are giant viruses and are present in high diversity in soil and water, and can infect protozoa and amoebas (Abergel et al, 2015;Aherfi et al, 2016;Colson et al, 2017;Andradedos et al, 2018;Schulz et al, 2018;Akashi and Takemura, 2019;Tokarz-DeptułaBeata et al, 2019).…”