A Novel Amperometric Glutamate Biosensor Based on Glutamate Oxidase Adsorbed on Silicalite

Abstract

In this work, we developed a new amperometric biosensor for glutamate detection using a typical method of glutamate oxidase (GlOx) immobilization via adsorption on silicalite particles. The disc platinum electrode (d = 0.4 mm) was used as the amperometric sensor. The procedure of biosensor preparation was optimized. The main parameters of modifying amperometric transducers with a silicalite layer were determined along with the procedure of GlOx adsorption on this layer. The biosensors based on GlOx adsorbed on silicalite demonstrated high sensitivity to glutamate. The linear range of detection was from 2.5 to 450 μM, and the limit of glutamate detection was 1 μM. It was shown that the proposed biosensors were characterized by good response reproducibility during hours of continuous work and operational stability for several days. The developed biosensors could be applied for determination of glutamate in real samples.

Design of Immobilized Enzyme Biocatalysts: Drawbacks and Opportunities

“…157 The kinetics of Michaelis-Menten indicate that the affinity, with respect to the type of conformation of the enzyme-substrate complex, 158,159 enabling better enantioselectivity of immobilized 6 His-CALB-10Lys enzymes in relation to other enzymes commercial in the resolution of (S)-N-(2ethyl-6-methylphenyl) alanine ((S)-NEMPA). 156 In the work of Soldatkina et al, 160 a new amperometric biosensor was developed for the detection of glutamate by the immobilization of glutamate oxidase (GlOx) on silicalite particles by adsorption. Biosensors of glutamate oxidase or decarboxylase adsorbed on silica showed high sensitivity to glutamate.…”

“…Biosensors of glutamate oxidase or decarboxylase adsorbed on silica showed high sensitivity to glutamate. 142,160 These biosensors exhibit excellent specificity, being able to detect glutamate in small amounts, reproducibility of results for several hours and stability to operate for several days. 143 Silica nanoparticles can be used as support for photochromic derivatives of 1-vinylidene-naphtho furan by direct adsorption and covalent interaction.…”

Electrochemiluminescence Biosensors Using Screen-Printed Electrodes

“…There are many nanomaterials used now for the development of enzymatic biosensors and their nature is broad, such as metallic nanoparticles of silver (AgNPs) [ 127 ] and gold (AuNPs) [ 128 ]; carbon nanomaterials as carbon nanodots (CNDs) [ 112 ], graphene [ 129 ] and carbon nanotubes (CNTs) [ 130 ]; and biochar [ 131 ]. Semiconductors such as quantum dots (QDs) [ 132 ] and silicates [ 133 ] are also investigated. All these nanomaterials can be deposited on the SPE and conventional electrodes surface for using as sensor platforms.…”

Flexible and wearable electrochemical biosensors based on two-dimensional materials: Recent developments

“…The electrochemical biosensor monitors the electrochemical signal produced by the reaction between the analyte molecules (target molecules) and the sensing probe [ 11 ]. The benefit of electrochemical biosensors is that different electrochemical techniques can be used based on the target molecules and environment such as potentiometry, amperometry, electrochemical impedance spectroscopy, and cyclic voltammetry [ 12 , 13 ]. Moreover, these electrochemical biosensors are unparalleled and suited for the design and manufacture of such flexible and wearable sensors due to their various advantages, including convenient operation, fast response, portability, and inherent miniaturization [ 14 ].…”