A novel analytical probe binding to a potential carcinogenic factor of N-glycolylneuraminic acid by SELEX

Gong, Sheng; Ren, Hong-Lin; Tian, Rui-Yun; Lin, Clement; Hu, Pan; Li, Yansong; Liu, Zengshan; Song, Jié; Tang, Feng; Zhou, Yu; Li, Zhaohui; Zhang, Yuanyuan; Lu, Shiying

doi:10.1016/j.bios.2013.05.024

Cited by 20 publications

(10 citation statements)

References 23 publications

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“…One of the aptamers produced a high affinity constant (K A ) of 6.68 Â 10 9 M À1 . 153 The technology was later developed into aptamernanoparticle biosensor strips for visual detection of Neu5Gc. 154 Both of the examples above highlight the latest use of aptamers as novel probes targeting sialic acids, for rapid and effective sensing of this particular type of glycan.…”

Section: Aptamersmentioning

confidence: 99%

The challenges of glycan recognition with natural and artificial receptors

et al. 2019

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Section: Aptamersmentioning

confidence: 99%

The challenges of glycan recognition with natural and artificial receptors

et al. 2019

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“…Generally, oligonucleotides fold into various secondary structures, such as stem-loop, pseudoknot, hairpin, pocket, and even G-quadruplexes, which act as the binding motifs or the region responsible for biological activity. The most likely minimum energy secondary structures of the selected aptamers were analyzed with the Mfold program to predict the structure or the aptamer that bound to the target toxin [33]. In this study, the secondary structures of most of the obtained aptamers were typical stem-loop (a) and hairpin-loop (b) structures; Figure 3 shows some of the aptamers.…”

Section: Resultsmentioning

confidence: 99%

“…The 5′-biotin-labeled products of the asymmetric PCR were used for an affinity analysis in each round. The dsDNA obtained from the round with the best affinity was used for cloning after it was purified according to the instructions of the Poly-Gel DNA Extraction Kit (OMEGA) [33]. …”

Section: Methodsmentioning

confidence: 99%

Preparation of a Specific ssDNA Aptamer for Brevetoxin-2 Using SELEX

Tian

Lin

et al. 2016

Journal of Analytical Methods in Chemistry

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The existing assays for detecting brevetoxin (BTX) depend on expensive equipment with a professional operator or on an antibody with limited stability, which requires complex processes, a high cost, and a considerable amount of time. The development of an alternative detection probe is another promising research direction. This paper reports the use of aptamers binding to BTX-2 in an analytical assay using the systematic evolution of ligands by exponential enrichment (SELEX). After 12 rounds of selection, the secondary structures of 25 sequences were predicted. Compared to other aptamers, Bap5 has relatively high affinity with the lowest dissociation constant of 4.83 μM, and IC50 is 73.81 ng mL−1. A good linear regression formula of y = 30.688x − 7.329 with a coefficient correlation of R 2 = 0.9798 was obtained using a biotin-avidin ELISA. Moreover, there is no cross-reaction with the detected marine toxins, except for BTX-2. Thus, Bap5 has potential to detect BTX-2 in shellfish in the future as a substitute for the recognition probe.

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“…In human body, the deletion of 92 bp fragment in exon results in the inactivation of CMAH [ 12 ]. In recent years, however, studies have revealed that Neu5Gc appears in human body in the form of exogenous [ 13 ], and it is closely related to a variety of cancers [ 14 ], such as colon cancer [ 15 ], bladder cancer [ 16 ], breast cancer, lung cancer, and ovarian cancer [ 17 ]. Under hypoxia, cancer cells can upregulate subunit B of respiratory complex II, i.e., an iron-sulfur [Fe2S2]-containing protein, making up for the lack of function of CMAH enzyme, and resulting in the high expression of Neu5Gc in cancer cells [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Evaluation of Tumor Development via Cellular Interface Supported CRISPR/Cas Trans-Cleavage

et al. 2022

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Evaluating tumor development is of great importance for clinic treatment and therapy. It has been known that the amounts of sialic acids on tumor cell membrane surface are closely associated with the degree of cancerization of the cell. So, in this work, cellular interface supported CRISPR/Cas trans-cleavage has been explored for electrochemical simultaneous detection of two types of sialic acids, i.e., N-glycolylneuraminic acid (Neu5Gc) and N-acetylneuraminic acid (Neu5Ac). Specifically, PbS quantum dot-labeled DNA modified by Neu5Gc antibody is prepared to specifically recognize Neu5Gc on the cell surface, followed by the binding of Neu5Ac through our fabricated CdS quantum dot-labeled DNA modified by Sambucus nigra agglutinin. Subsequently, the activated Cas12a indiscriminately cleaves DNA, resulting in the release of PbS and CdS quantum dots, both of which can be simultaneously detected by anodic stripping voltammetry. Consequently, Neu5Gc and Neu5Ac on cell surface can be quantitatively analyzed with the lowest detection limits of 1.12 cells/mL and 1.25 cells/mL, respectively. Therefore, a ratiometric electrochemical method can be constructed for kinetic study of the expression and hydrolysis of Neu5Gc and Neu5Ac on cell surface, which can be further used as a tool to identify bladder cancer cells at different development stages. Our method to evaluate tumor development is simple and easy to be operated, so it can be potentially applied for the detection of tumor occurrence and development in the future.

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A novel analytical probe binding to a potential carcinogenic factor of N-glycolylneuraminic acid by SELEX

Cited by 20 publications

References 23 publications

The challenges of glycan recognition with natural and artificial receptors

The challenges of glycan recognition with natural and artificial receptors

Preparation of a Specific ssDNA Aptamer for Brevetoxin-2 Using SELEX

Electrochemical Evaluation of Tumor Development via Cellular Interface Supported CRISPR/Cas Trans-Cleavage

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