A novel anti‐Thy‐1 (CD90) monoclonal antibody induces apoptosis in mouse malignant T‐lymphoma cells in spite of inducing bcl‐2 expression

Fujita, Naoya; Kato, Yoshinori; Naito, Mikihiko; Tsuruo, Takashi

doi:10.1002/(sici)1097-0215(19960516)66:4<544::aid-ijc20>3.3.co;2-c

Cited by 5 publications

(8 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…131 I (iodine 131) labeled anti-CD90-1.1 antibodies have been used by Badger et al for radiotherapy to treat murine lymphoma [126]. An anti-CD90 antibody MCS-34, which recognizes Thy-1.1 as well as Thy-1.2 epitopes, induced apoptosis in CS-21 mouse malignant T-lymphoma cell line, despite inducing Bcl-2 protein expression as tested by Fujita et al MCS-34 caused an increase in cytoplasmic calcium ion concentration leading to apoptosis of malignant Tlymphoma cells [39]. However, when Ishiura et al treated B-cell lymphoma cell lines Akata, BJAB, and MutuI with an anti-CD90 monoclonal antibody, it induced apoptosis more efficiently than rituximab through caspase activation and downregulation of Bcl-2 expression [107].…”

Section: Cd90 As Therapeutic Targetmentioning

confidence: 99%

“…CD90 expression was also observed in glioma cell lines (A172 MG and D54 MG), neuroblastomas, rhabdomyosarcoma, leiomyosarcoma (UCLA-SO-S1), and teratoma (Tera-1 and PA-1) [34]. The known functions of CD90 are T-cell activation [35], regulation of neurite outgrowth in neurons [24,[36][37][38], regulation of apoptosis in thymocytes [39][40][41] and mesangial cells [42,43], modulation of fibrosis, and promotion of cell migration, adhesion, and extravasation [44][45][46][47]. CD90 is known to cause Hep I-mediated phosphorylation of Src family kinase (SFK) and Focal adhesion kinase (FAK) followed by focal adhesion disassembly and migration [48].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Multiple roles of CD90 in cancer

et al. 2016

View full text Add to dashboard Cite

THY1 (CD90) is a 25-37-kDa heavily N-glycosylated, glycophosphatidylinositol (GPI) anchored cell surface protein. It is usually expressed on thymocytes, mesenchymal stem cells, hematopoietic stem cells, natural killer cells, neurons, endothelial cells, renal glomerular mesangial cells, follicular dendritic cells, fibroblasts, and myofibroblasts. It has been found to regulate cell adhesion, migration, apoptosis, axon growth, cell-cell and cell-matrix interactions, T-cell activation, and fibrosis. Several reports have shown that CD90 has an important role in cancer in regulating cancer cell proliferation, metastasis, and angiogenesis. There are also evidences that CD90 is an important prognostic marker in many cancers. Consequently, therapies that target CD90 have great promise in treating many cancers. However, several studies also indicate a contradictory role for CD90, where it acts as a tumor suppressor. In this review, we summarize the expression, function of CD90 in different cancers and its possible use as a biomarker or a therapeutic target in cancer. The challenges and future prospects for the use of CD90 for clinical applications are also discussed in this review.

show abstract

Section: Cd90 As Therapeutic Targetmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Multiple roles of CD90 in cancer

et al. 2016

View full text Add to dashboard Cite

show abstract

“…THY1 is expressed in various types of cells [9], such as thymus [16][17][18], T cells and B cells [11,12], neurons [19], fibroblasts [20], ovarian cells [21,22], endothelial cells [12], and kidney [23,24], in various animals, including mice [10,11,13,[15][16][17][18][19]25], rats [9,10,20,[22][23][24], and humans [10,21]. THY1 has been reported to be involved in various functions, such as proliferation and apoptosis [17,23,24,26] as well as adhesion [12,16,19,20,27,28]. Of these suggested functions, cell adhesion is one that has gained general acceptance [12].…”

Section: Introductionmentioning

confidence: 99%

The Roles of THY1 and Integrin Beta3 in Cell Adhesion During Theca Cell Layer Formation and the Effect of Follicle-Stimulating Hormone on THY1 and Integrin Beta3 Localization in Mouse Ovarian Follicles

Itami

Tamotsu

Sakai

et al. 2011

Biology of Reproduction

View full text Add to dashboard Cite

The mechanism of theca cell layer formation in mammalian ovaries has not been elucidated. In the present study, we examined the roles of THY1 and integrin beta3 in theca cell layer formation during mouse folliculogenesis. The localization pattern of THY1 and integrin beta3 in adult mouse ovary was investigated immunohistochemically. The strongest THY1 signal was observed in theca cell layers from secondary to preantral follicles, at which time theca cells have begun to participate in follicle formation. Integrin beta3 also localized to the theca cell layer of secondary to preantral follicles and showed a localization pattern similar to that of THY1. Moreover, the role of THY1 in theca cell layer formation was examined using a follicle culture system. When anti-THY1 antibody was added to this culture, no theca cell layers were formed, and the granulosa cells were distanced from each other. Because a THY1 signal was not observed in ovaries at stages earlier than prepuberty, THY1 localization also appeared to be affected by mouse development. This possibility was examined by determining the effect of administering follicle-stimulating hormone, luteinizing hormone, and 17beta-estradiol to 7-day-old mice on THY1 localization in the ovary 3 days later. Only follicle-stimulating hormone induced a THY1 signal in 10-day-old mouse ovaries. No THY1 signal was observed in untreated 10-day-old ovaries. In conclusion, THY1 might play a role in cell adhesion via binding to integrin beta3 in mouse ovaries. The present results suggest that THY1 localization may be affected by follicle-stimulating hormone in mouse ovaries.

show abstract

“…Thy-1 can signal via integrins, focal adhesion kinase (FAK), and Rho to mediate cell adhesion [26,27]. Thy-1 can also activate cell death and inhibit tumorigenic growth of cancer cells [28][29][30][31][32][33][34][35]. Expression of Thy-1 modulates the proliferative responses of fibroblasts to cytokines and growth factors [36][37][38][39][40].…”

Section: Introductionmentioning

confidence: 99%

Thy-1, a versatile modulator of signaling affecting cellular adhesion, proliferation, survival, and cytokine/growth factor responses

Rege

Hagood

2006

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research

162

141

View full text Add to dashboard Cite

Thy-1 is a 25-37 kDa glycosylphosphatidylinositol (GPI)-anchored protein involved in T cell activation, neurite outgrowth, apoptosis, tumor suppression, wound healing, and fibrosis. To mediate these diverse effects, Thy-1 participates in multiple signaling cascades. In this review, we discuss Thy-1 signaling primarily in non-immunologic cell types, including neurons, mesangial cells, ovarian cancer cells, nasopharyngeal carcinoma cells, endothelial cells, and fibroblasts. We review the current literature regarding Thy-1 signaling via integrins, protein tyrosine kinases, and cytokines and growth factors; and the roles of these signaling pathways in cellular adhesion, apoptosis, cell proliferation, and cell adhesion and migration. We also discuss the role of Thy-1 localization to lipid rafts, and of the GPI anchor in Thy-1 signaling. Ongoing research on the mechanisms of Thy-1 signaling will add to our understanding of the diverse physiologic and pathologic processes in which Thy-1 plays a role.

show abstract

A novel anti‐Thy‐1 (CD90) monoclonal antibody induces apoptosis in mouse malignant T‐lymphoma cells in spite of inducing bcl‐2 expression

Cited by 5 publications

References 1 publication

Multiple roles of CD90 in cancer

Multiple roles of CD90 in cancer

The Roles of THY1 and Integrin Beta3 in Cell Adhesion During Theca Cell Layer Formation and the Effect of Follicle-Stimulating Hormone on THY1 and Integrin Beta3 Localization in Mouse Ovarian Follicles

Thy-1, a versatile modulator of signaling affecting cellular adhesion, proliferation, survival, and cytokine/growth factor responses

Contact Info

Product

Resources

About