A novel antimicrobial peptide from amphibian skin secretions of Odorrana grahami

Che, Qiaolin; Zhou, Yu; Yang, Hailong; Li, Jianxu; Xu, Xueqing; Lai, Ren

doi:10.1016/j.peptides.2008.01.004

Cited by 41 publications

(33 citation statements)

References 19 publications

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“…The use of antimicrobial peptides by many organisms as part of their innate defense system against harmful invading microorganisms is widely recognized (Che et al, 2008). One peculiar characteristic of such peptides is their broad spectrum of action against different types of bacteria, as well as some fungi.…”

Section: Introductionmentioning

confidence: 99%

“…The impact of such a challenging problem has motivated numerous academic and pharmaceutical industry research groups to develop new drugs capable of dealing with the adaptation strategy (namely the selection of organisms adapted to some specific medium) that these organisms develop over time (Prates and Bloch Júnior, 2000;Fazio et al, 2006;Ramamoorthy et al, 2006;Che et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

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The role of disulfide bridges in the 3-D structures of the antimicrobial peptides gomesin and protegrin-1: a molecular dynamics study

Castro¹,

Fuzo²,

Degrève³

et al. 2008

Genet. Mol. Res.

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ABSTRACT. Some antimicrobial peptides have a broad spectrum of action against many different kinds of microorganisms. Gomesin and protegrin-1 are examples of such antimicrobial peptides, and they were studied by molecular dynamics in this research. Both have a β-hairpin conformation stabilized by two disulfide bridges and are active against Gram-positive and Gram-negative bacteria, as well as fungi. In this study, the role of the disulfide bridge in the maintenance of the tertiary peptide structure of protegrin-1 and gomesin is analyzed by the structural characteristics of these peptides and two of their respective variants, gomy4 and proty4, in which the four cysteines are replaced by four tyrosine residues. The absence of disulfide bridges in gomy4 and proty4 is compensated by overall reinforcement of the original hydrogen bonds and extra attractive interactions between the aromatic rings of the tyrosine residues. The net effects on the variants with respect to the corresponding natural peptides are: i) maintenance of the original β-hairpin conformation, with great structural similarities between the mutant and the corresponding natural peptide; ii) combination of positive Φ and Ψ Ra- Role of disulfide bridges in gomesin and protegrin-1 machandran angles within the hairpin head region with a qualitative change to a combination of positive (Φ) and negative (Ψ) angles, and iii) significant increase in structural flexibility. Experimental facts about the antimicrobial activity of the gomesin and protegrin-1 variants have also been established here, in the hope that the detailed data provided in the present study may be useful for understanding the mechanism of action of these peptides.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The role of disulfide bridges in the 3-D structures of the antimicrobial peptides gomesin and protegrin-1: a molecular dynamics study

Castro¹,

Fuzo²,

Degrève³

et al. 2008

Genet. Mol. Res.

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“…Consequently, amphibian bioactive peptides exhibit biological diversity. More than 110 polypeptides with antimicrobial and/or protease inhibitor activity were isolated from the skin of Rana graham, [12][13][14][15] and 197 bioactive peptides were identified from Odorrana andersonii by screening of a cDNA library. 16) These abundant bioactive compounds were suggested to be the products of adaptation to inhospitable surroundings, but the variability of the bioactive peptide repertoires of individual species remains unclear.…”

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confidence: 99%

Different Expression Profiles of Bioactive Peptides inPelophylax nigromaculatusfrom Distinct Regions

Song

Liu

et al. 2013

Bioscience, Biotechnology, and Biochemistry

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“…In other studies, to obtain the bioactive compounds from frog skin secretions (Woodhams et al, 2007) immersed frogs in norephinephrine bath for 15 min. Some other techniques that have been employed for releasing frog skin secretion include parotid gland squeezing (Garg et al, 2007), ether inhydrate exposure (Che et al, 2008) and electric shock (Barlian et al, 2011). This suggests that the accumulation of frog skin secretion can be carried out using different approaches depending on the purpose of the study.…”

Section: Discussionmentioning

confidence: 99%

Antifungal Activity of Skin Secretion of Bleeding Toad <i>Leptophryne Cruentata</i> and Javan Tree Frog <i>Rhacophorus Margaritifer</i>

Artika¹,

Pinontoan²,

Kusrini³

2015

American Journal of Biochemistry and Biotechnology

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Amphibian skin has been the source of a wide variety of biologically active compounds. This study was aimed to determine antifungal activity of skin secretion of two species of anurans endemic in Indonesia, the bleeding toad Leptophryne cruentata and the javan tree frog Rhacophorus margaritifer. We collected 7 adults of L. cruentata and 10 adults of R. margaritifer from the wild in Mount Gede Pangrango National Park, West Java. The toads and frogs were induced to release skin secretion using a nonlethal induction technique based on epinephrine injection. The collected skin secretion was filter-sterilized, freeze-dried and subjected to antifungal assay. Results showed that skin secretion of some toads and frogs has antifungal activity against the fungus Trichophyton mentagrophytes. Among the chemical compounds with potential bioactivity detected in the skin secretion include fatty acids, amines and steroids. Therefore, the toad and frog skin secretion has the potential to be developed as a source of antifungal agents.

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A novel antimicrobial peptide from amphibian skin secretions of Odorrana grahami

Cited by 41 publications

References 19 publications

The role of disulfide bridges in the 3-D structures of the antimicrobial peptides gomesin and protegrin-1: a molecular dynamics study

The role of disulfide bridges in the 3-D structures of the antimicrobial peptides gomesin and protegrin-1: a molecular dynamics study

Different Expression Profiles of Bioactive Peptides inPelophylax nigromaculatusfrom Distinct Regions

Antifungal Activity of Skin Secretion of Bleeding Toad <i>Leptophryne Cruentata</i> and Javan Tree Frog <i>Rhacophorus Margaritifer</i>

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