A novel approach of human embryonic stem cells therapy in treatment of Friedrich's Ataxia

Shroff, Geeta

doi:10.5348/ijcri-201503-cs-10054

Cited by 16 publications

(13 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Many studies on embryonic stem cells derived from rats report the use of ESCs in treatment of injuries related to brain and spinal cord [24,25]. The effect of hESC therapy in patients with cerebral palsy, cortico-visual impairment, Friedrich ataxia and spino-cerebral ataxia has also been previously reported by the authors [17,[26][27][28].…”

Section: Discussionmentioning

confidence: 92%

Role of Anesthetists in Human Embryonic Stem Cells Transplantation in Patients with Spinal Cord Injury

Sonowal¹

2015

J Anesth Clin Res

View full text Add to dashboard Cite

Spinal cord injury (SCI) is a devastating disorder affecting millions across the world. Still, there is no standard approach for the treatment of SCI. The concept of regenerative medicine using the stem cells to repair tissues has become a reality of new era. Human embryonic stem cells (hESCs) have boosted as a new therapeutic strategy to treat SCI. The implantation of hESCs with reduced pain is the major concern for physicians. Our institute has been using specialized procedures to implant hESCs in a SCI patient via anesthesia techniques. The implantation of hESCs as well as the induction of anesthesia requires an employment of a skilled anesthetist. The authors focus on the novel approach, i.e., use of epidural and caudal routes for introduction of hESCs, as well as the role of an anesthetist in implantation of hESCs in the patients of SCI. An epidural injects hESCs into the region outside the duramater of the meninges whereas; hESCs implantation through the sacral membrane which is approximately three centimeters above the tip of the coccyx and is in continuum with the epidural space is achieved by caudal route. An anesthetist has an edge over the others due to his skills and knowledge regarding anesthetic agents, routes of administration as well as pain controlling strategy. He plays a significant role in hESC transplantation by evaluating a patient's condition, protocol development along with a suitable health care management. Anesthetists might contribute to comprehensive patient care by encouraging the optimal use of multimodal regimens, as well as in implementing novel techniques, ensuring improvement in pain control and minimize adverse events.

show abstract

Section: Discussionmentioning

confidence: 92%

Role of Anesthetists in Human Embryonic Stem Cells Transplantation in Patients with Spinal Cord Injury

Sonowal¹

2015

J Anesth Clin Res

View full text Add to dashboard Cite

show abstract

“…The development of neuronal tissue differentiation protocols will expand the application of hESCs-derived NPCs to neurological diseases. The stem cells used in our study have been injected into patients suffering from various neurodegenerative diseases such as cerebral palsy, spinal cord injury and Friedreich’s ataxia and have shown successful results[11,21-23]. …”

Section: Discussionmentioning

confidence: 99%

Morphogenesis of human embryonic stem cells into mature neurons under in vitro culture conditions

Shroff¹

2016

WJEM

Self Cite

View full text Add to dashboard Cite

AIMTo describe the morphogenesis of different neuronal cells from the human embryonic stem cell (hESC) line, SCT-N, under in vitro culture conditions.METHODSThe directed neuronal cell line was produced from a single, spare, pre-implantation stage fertilized ovum that was obtained during a natural in vitro fertilization process. The hESCs were cultured and maintained as per our proprietary in-house technology in a Good Manufacturing Practice, Good Laboratory Practice and Good Tissue Practice compliant laboratory. The cell line was derived and incubated in aerobic conditions. The cells were examined daily under a phase contrast microscope for their growth and differentiation.RESULTSDifferent neural progenitor cells (NPCs) and differentiating neurons were observed under the culture conditions. Multipotent NPCs differentiated into all three types of nervous system cells, i.e., neurons, oligodendrocytes and astrocytes. Small projections resembling neurites or dendrites, and protrusion coming out of the cells, were observed. Differentiating cells were observed at day 18 to 20. The differentiating neurons, neuronal bodies, axons, and neuronal tissue were observed on day 21 and day 30 of the culture. On day 25 and day 30, prominent neurons, axons and neuronal tissue were observed under phase contrast microscopy. 4’, 6-diamidino-2-phenylindole staining also indicated the pattern of differentiating neurons, axonal structure and neuronal tissue.CONCLUSIONThis study describes the generation of different neuronal cells from an hESC line derived from biopsy of blastomeres at the two-cell cleavage stage from a discarded embryo.

show abstract

“…We have previously demonstrated the safety and efficacy of hESC therapy in patients with cerebral palsy [12], cortico-visual impairment [13] and Friedrich ataxia [14]. We have also reported some patients who hESC for Spinal Cord Injury J Neurol Res.…”

Section: Introductionmentioning

confidence: 91%

Human Embryonic Stem Cells in Treatment of Spinal Cord Injury: A Prospective Study

Shroff¹,

Agarwal

Mishra³

et al. 2015

J Neurol Res

Self Cite

View full text Add to dashboard Cite

show abstract

A novel approach of human embryonic stem cells therapy in treatment of Friedrich's Ataxia

Cited by 16 publications

References 21 publications

Role of Anesthetists in Human Embryonic Stem Cells Transplantation in Patients with Spinal Cord Injury

Role of Anesthetists in Human Embryonic Stem Cells Transplantation in Patients with Spinal Cord Injury

Morphogenesis of human embryonic stem cells into mature neurons under in vitro culture conditions

Human Embryonic Stem Cells in Treatment of Spinal Cord Injury: A Prospective Study

Contact Info

Product

Resources

About