A Novel Approach to Enhance the Urease Activity of <i>Sporosarcina pasteurii</i> and its Application on Microbial-Induced Calcium Carbonate Precipitation for Sand

Zhao, Yunfeng; Xiao, Zhiyang; Lv, Jie; Shen, Wanqing; Xu, Rongchao

doi:10.1080/01490451.2019.1631911

Cited by 27 publications

(27 citation statements)

References 22 publications

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“…Urease activity is considered to be a key factor for MICP performance ( Konstantinou et al, 2021 ). Multiple studies use different techniques to increase the urease activity of a bacterial culture ( Achal et al, 2009 ; Omoregie et al, 2017 ; Zhao et al, 2019 ), or isolate new ureolytic microorganisms ( Li et al, 2013 ; Krishnapriya et al, 2015 ; Kim et al, 2016 ; Zhao et al, 2017 ). Quantification of urease activity plays an important role for MICP research and application ( Cui et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Developing a fluorometric urease activity microplate assay suitable for automated microbioreactor experiments

Lapierre

Bolz

Büchs

et al. 2022

Front. Bioeng. Biotechnol.

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Quantifying urease activity is an important task for Microbial Induced Calcite Precipitation research. A new urease activity microplate assay using a fluorescent pH indicator is presented. The method is also suitable for automated measurements during microbioreactor experiments. The assay reagent consists of the green fluorescent pH-indicator fluorescein, urea and a phosphate buffer. After sample addition, the microbial urease hydrolyses urea, which results in a pH and hence fluorescence increase. The fluorescence signal can be measured with a microplate reader or with the microbioreactor system BioLector, allowing for automated urease activity measurements during cultivation experiments. In both measurement systems, the fluorescence signal slope highly correlates with the urease activity measured offline with standard methods. Automated measurement is possible, as no sample preparation such as centrifugation or adjusting of the optical density is required. The assay was developed so that the culture samples turbidity, salinity or buffer concentration does not have a negative impact on the fluorescence signal. The assay allows for straightforward, non-hazardous, parallelized, cheap and reliable measurements, making research on ureolytic bacteria for Microbial Induced Calcite Precipitation more efficient. The assay could be adapted to other enzymes, which have a strong impact on the pH value.

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Section: Introductionmentioning

confidence: 99%

Developing a fluorometric urease activity microplate assay suitable for automated microbioreactor experiments

Lapierre

Bolz

Büchs

et al. 2022

Front. Bioeng. Biotechnol.

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“…Therefore, urease-producing bacteria (Pasteurella subtilis, Sporosarcina, Bacillus subtilis, and Bacillus megaterium) are known to secrete urease during metabolism to accelerate the hydrolysis of urea. Although large-scale production of highly active urease-producing bacteria is costly and affects the precipitation process [ 102 ], many researchers selected suitable media to enhance urease activity for MICP by indoor culture [ 24 , 25 , 103 ]. Hydrolysis of urea by urease-producing bacteria to induce calcium carbonate precipitation has more advantages than other production routes, such as simple mechanism, low cost, green and environmental protection, and the ability to produce a considerable amount of calcium carbonate precipitation in a short period of time ( Fig 3 ) [ 104 ].…”

Section: Mineralized Bacteriamentioning

confidence: 99%

Research status and development of microbial induced calcium carbonate mineralization technology

et al. 2022

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In nature, biomineralization is a common phenomenon, which can be further divided into authigenic and artificially induced mineralization. In recent years, artificially induced mineralization technology has been gradually extended to major engineering fields. Therefore, by elaborating the reaction mechanism and bacteria of mineralization process, and summarized various molecular dynamics equations involved in the mineralization process, including microbial and nutrient transport equations, microbial adsorption equations, growth equations, urea hydrolysis equations, and precipitation equations. Because of the environmental adaptation stage of microorganisms in sandy soil, their reaction rate in sandy soil environment is slower than that in solution environment, the influencing factors are more different, in general, including substrate concentration, temperature, pH, particle size and grouting method. Based on the characteristics of microbial mineralization such as strong cementation ability, fast, efficient, and easy to control, there are good prospects for application in sandy soil curing, building improvement, heavy metal fixation, oil reservoir dissection, and CO2 capture. Finally, it is discussed and summarized the problems and future development directions on the road of commercialization of microbial induced calcium carbonate precipitation technology from laboratory to field application.

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“…Moreover, Rowshanbakht et al [201] reported that reducing the injected volume of bacteria solution to up to one-third of the pore volume did not significantly affect the performance improvement of the MICP treatment. On the other hand, percolating more bacterial cells into the soil may lead to clogging at the soil surface, resulting in the inhomogeneous distribution of the precipitated calcium carbonate [209].…”

Section: Strength Enhancement Of Soilmentioning

confidence: 99%

“…Organic waste can be a nutrient source for both bacteria growth and fermenting microorganisms in large-scale applications to diminish the cost. Some studies proposed to cultivate the bacteria in non-sterile conditions [45,209] or using inexpensive technical-grade reagents [306], food-grade yeast medium [307], and sanitized media with inexpensive nutrients and water resources [308] to reduce the cost for the in situ application. Using limestone powder derived from aggregate quarries and disposed of industrial biofuel acid to produce calcium source for biocementation can be a cost-effective and sustainable method [126].…”

Section: Challenges and Strategies For Biogeotechnologymentioning

confidence: 99%

Recent development in biogeotechnology and its engineering applications

Cui

Chu

2021

Front. Struct. Civ. Eng.

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Microbial geotechnology or biogeotechnology is a new branch of geotechnical engineering. It involves the use of microbiology for traditional geotechnical applications. Many new innovative soil improvement methods have been developed in recent years based on this approach. A proper understanding of the various approaches and the performances of different methods can help researchers and engineers to develop the most appropriate geotechnical solutions. At present, most of the methods can be categorized into three major types, biocementation, bioclogging, and biogas desaturation. Similarities and differences of different approaches and their potential applications are reviewed. Factors affecting the different processes are also discussed. Examples of up-scaled model tests and pilot trials are presented to show the emerging applications. The challenges and problems of biogeotechnology are also discussed.

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A Novel Approach to Enhance the Urease Activity of Sporosarcina pasteurii and its Application on Microbial-Induced Calcium Carbonate Precipitation for Sand

Cited by 27 publications

References 22 publications

Developing a fluorometric urease activity microplate assay suitable for automated microbioreactor experiments

Developing a fluorometric urease activity microplate assay suitable for automated microbioreactor experiments

Research status and development of microbial induced calcium carbonate mineralization technology

Recent development in biogeotechnology and its engineering applications

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