A novel aptamer-mediated CuInS2quantum dots@graphene oxide nanocomposites-based fluorescence “turn off–on” nanosensor for highly sensitive and selective detection of kanamycin
Abstract:A novel aptamer-mediated fluorescence “turn off–on” nanosensor for highly sensitive and selective detection of kanamycin using CuInS2quantum dots@graphene oxide.
“…Similar to the result of detection in buffer, The LOD calculated using the 3-sigma method in the range of 10–50 ng/mL were 6.562, 6.179, and 6.616 ng/mL for T1, T2, and T3, respectively (Table 1). Additionally, in the milk samples, the aptasensor successfully detected antibiotics with low LOD values that were lower than the respective MRL values for sulfadimethoxine, kanamycin and ampicillin in milk 46–48 .Figure 5Quantitative detection of spiked antibiotics in milk sample. Shown are the fluorescence intensity measured from 10 ng/mL to 500 ng/mL.…”
The development of a multiplexed sensing platform is necessary for highly selective, sensitive, and rapid screening of specific antibiotics. In this study, we designed a novel multiplex aptasensor for antibiotics by fluorescence resonance energy transfer (FRET) strategy using DNase I-assisted cyclic enzymatic signal amplification (CESA) method combined with aptamer/graphene oxide complex. The aptamers specific for sulfadimethoxine, kanamycin, and ampicillin were conjugated with Cyanine 3 (Cy3), 6-Carboxyfluorescein (FAM), and Cyanine 5 (Cy5), respectively, and graphene oxide (GO) was adopted to quench the fluorescence of the three different fluorophores with the efficiencies of 94.36%, 93.94%, and 96.97% for Cy3, FAM, and Cy5, respectively. CESA method was used for sensitive detection, resulting in a 2.1-fold increased signal compared to those of unamplified method. The aptasensor rapidly detected antibiotics in solution with limit of detection of 1.997, 2.664, and 2.337 ng/mL for sulfadimethoxine, kanamycin, and ampicillin, respectively. In addition, antibiotics dissolved in milk were efficiently detected with similar sensitivities. Multiplexed detection test proved that the fluorescently modified aptamers could work separately from each other. The results indicate that the aptasensor offers high specificity for each antibiotic and enables simultaneous and multicolor sensing for rapid screening of multiple antibiotics at the same time.
“…Similar to the result of detection in buffer, The LOD calculated using the 3-sigma method in the range of 10–50 ng/mL were 6.562, 6.179, and 6.616 ng/mL for T1, T2, and T3, respectively (Table 1). Additionally, in the milk samples, the aptasensor successfully detected antibiotics with low LOD values that were lower than the respective MRL values for sulfadimethoxine, kanamycin and ampicillin in milk 46–48 .Figure 5Quantitative detection of spiked antibiotics in milk sample. Shown are the fluorescence intensity measured from 10 ng/mL to 500 ng/mL.…”
The development of a multiplexed sensing platform is necessary for highly selective, sensitive, and rapid screening of specific antibiotics. In this study, we designed a novel multiplex aptasensor for antibiotics by fluorescence resonance energy transfer (FRET) strategy using DNase I-assisted cyclic enzymatic signal amplification (CESA) method combined with aptamer/graphene oxide complex. The aptamers specific for sulfadimethoxine, kanamycin, and ampicillin were conjugated with Cyanine 3 (Cy3), 6-Carboxyfluorescein (FAM), and Cyanine 5 (Cy5), respectively, and graphene oxide (GO) was adopted to quench the fluorescence of the three different fluorophores with the efficiencies of 94.36%, 93.94%, and 96.97% for Cy3, FAM, and Cy5, respectively. CESA method was used for sensitive detection, resulting in a 2.1-fold increased signal compared to those of unamplified method. The aptasensor rapidly detected antibiotics in solution with limit of detection of 1.997, 2.664, and 2.337 ng/mL for sulfadimethoxine, kanamycin, and ampicillin, respectively. In addition, antibiotics dissolved in milk were efficiently detected with similar sensitivities. Multiplexed detection test proved that the fluorescently modified aptamers could work separately from each other. The results indicate that the aptasensor offers high specificity for each antibiotic and enables simultaneous and multicolor sensing for rapid screening of multiple antibiotics at the same time.
“…[ 29,30,33,38 ] Usually, these fluorescent nanoprobes act as fluorescence sensors based on different strategies and modes. The most important of which include quenching (turn off), [ 31,39 ] enhancement (turn on), [ 40–42 ] quenching–recovery (turn off–on), [ 43–49 ] turn on–off, [ 50 ] turn on–off–on, [ 51,52 ] turn off–on–off [ 53 ] and metal enhanced fluorescence (MEF). [ 54 ]…”
Section: Introductionmentioning
confidence: 99%
“…Among these different modes, the fluorescence turn off–on model has been widely used to measure different analytes. [ 27–30,33,43–49 ] In this sensing model, fluorescent nanomaterials are commonly used as optical sensors in which competing analytes with strong interaction affinities for quenching agents are used. As a result of the strong and specific interactions between them, the quenched fluorescence of system (turn off) is gradually restored (recovery/turn on) and a turn off–on detection signal is created.…”
Metformin (MET), as an oral antidiabetic and antihyperglycemic agent, is widely used to treat type II diabetes mellitus. Because of its increasing consumption, developing a fast, simple, and selective method to determine its concentration in biological samples (serum and urine) and pharmaceutical formulations (tablets) is of great interest. In this study, we used a FRET‐based fluorescent nanosensor (Tb–phen–AgNPs system) for sensitive detection of MET in tablet and serum samples. This method is based on the enhancing effect of MET on the emission intensity of the Tb–phen complex, which is quenched by AgNPs via energy transfer process (turn off–on mode). A good linear relationship between the MET concentration and enhanced emission intensity of the Tb–phen–AgNPs system was observed in the range of (0.75–3.7) × 10−6 M under optimum conditions. Limit of detection and limit of quantitation were calculated to be 0.43 × 10−6 M and 1.31 × 10−6 M, respectively. This method was successfully used to determine MET concentrations in pharmaceutical dosage form and in spiked serum sample. The obtained recoveries from pharmaceutical formulation and serum sample were in the range 86.75–98.97% and 85.10–100.96%, respectively. Collectively, our results indicated that the method described here is simple, sensitive, cost effective, and free from interference. Therefore, it can be used as an effective and routine method for the direct and rapid determination of MET levels in biological samples such as serum.
“…8,9 Consequently, aptamers are gaining popularity in the eld of biosensing. 10 Since the discovery of the kanamycin binding aptamer (KBA), 11 there have been several attempts to develop KBA based sensors for the detection of residual kanamycin using different optical [12][13][14][15][16][17][18][19][20][21] and electrochemical [22][23][24][25][26][27][28][29][30][31][32][33] schemes. Out of these, uorescent aptasensors have several advantages due to their simpler implementation schemes and high sensitivity.…”
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