A novel automated transplanting system for plant tissue culture

Lee, Tien Jung; Zobayed, Sma; Firmani, Flavio; Park, Edward J.

doi:10.1016/j.biosystemseng.2019.02.012

Cited by 10 publications

(3 citation statements)

References 4 publications

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“…These variables achieve high proliferation rates, but they also frequently result in physiological, anatomical, and morphological disorders that impede ex vitro acclimatization and decrease the survival rate of plants [72,73]. High proliferation rates during the in vitro multiplication stage, successful ex vitro acclimatization of the plantlets, high plant survival rates, and some degree of automation are all necessary for effective commercial in vitro propagation [74]. Successful acclimatization creates the ideal conditions for greater survival, subsequent growth, and the establishment of in vitro raised plantlets.…”

Section: Discussionmentioning

confidence: 99%

Design and Evaluation of a Smart Ex Vitro Acclimatization System for Tissue Culture Plantlets

2022

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One of the technological advancements in agricultural production is the tissue culture propagation technique, commonly used for mass multiplication and disease-free plants. The necessity for date palm tissue culture emerged from the inability of traditional propagation methods’ offshoots to meet the immediate demands for significant amounts of planting material for commercial cultivars. Tissue culture plantlets are produced in a protected aseptic in vitro environment where all growth variables are strictly controlled. The challenges occur when these plantlets are transferred to an ex vitro climate for acclimatization. Traditional glasshouses are frequently used; however, this has substantial mortality consequences. In the present study, a novel IoT-based automated ex vitro acclimatization system (E-VAS) was designed and evaluated for the acclimatization of date palm plantlets (cv. Khalas) to enhance their morpho-physiological attributes and reduce the mortality rate and the contamination risk through minimal human contact. The experimental findings showed that the morpho-physiological parameters of 6- and 12-month-old plants were higher when acclimatized in the prototype E-VAS compared to the traditional glasshouse acclimatization system (TGAS). The maximum plant mortality percentage occurred within the first month of the transfer from the in vitro to ex vitro environment in both systems, which gradually declined up to six months; after that, no significant plant mortality was observed. About 6% mortality was recorded in E-VAS, whereas 18% in TGAS within the first month of acclimatization. After six months of study, an overall 14% mortality was recorded in E-VAS compared to 41% in TGAS. The proposed automated system has a significant potential to address the growing demand for the rapid multiplication of tissue culture-produced planting materials since the plant survival rate and phenotype quality were much higher in E-VAS than in the conventional manual system that the present industry follows for commercial production.

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Section: Discussionmentioning

confidence: 99%

Design and Evaluation of a Smart Ex Vitro Acclimatization System for Tissue Culture Plantlets

2022

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“…However only a little amount of study has been done on the application of this technique at the plantlet acclimation stage, where the death rate is quite significant. Effective commercial in vitro propagation requires a combination of factors, including high plant proliferation rates during the in vitro multiplication stage, high plant survival rates, and some level of automation (Lee et al, 2019). The optimum environment is set up by a successful acclimatization.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Propagation of Date Palm (Phoenix Dactylifera L.) “Lakramat” Date Palms in Aswan Governorate

Abd Elaziem

2023

Egyptian International Journal of Palms

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Lakramat cultivar is mainly cultivated in the Siwa Oasis. It is one of the promising Egyptian date palm cultivars, and its good traits make it appropriate for export. This paper describes the micropropagation process utilizing Lakramat cv. shoot tip explants. Explants were grown for 36 weeks on MS medium supplemented with picloram at 2.0 mg/l mixed with 2iP at 0.5 mg/l and 0.5 g/l AC for initiation stage. The obtained embryonic cultures were placed for further development and embryo formation in maturation media containing 0.25 mg/l picloram in combination with 2iP at 1.0 mg/l. The obtained somatic embryos were shifted to multiplication medium containing putrescine at the concentrations of 0, 50, 100, and 150 mg/l to study its effect on multiplication rate. Various levels of light intensity during germination stage were also investigated. Indeed, a new system of acclimatization was discussed. Date palm (Phoenix dactylifera L.) -Lakramat‖ direct globular somatic embryos spontaneously formed on shoot tip sections. The most successful somatic embryo multiplication was produced during treatment with 100 mg/l Putrescine. On the other side, the higher intensity (6000 lux) promoted germination and shoot formation. During rooting stage, the addition of 1.5 mg/l IBA and 1mg/l NAA in rooting medium achieved the highest significant root number. New system of acclimatization used in the present protocol surpassed the traditional methods of acclimatization. The maximum levels of ex vitro plant growth vigor, leaf number/plant, leaf width, root number, and root thickness were stimulated by irrigation with the MS full strength solution.

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“…Propagation through tissue culture also eliminates the possibility of any interruption in the growing season because it can be carried out inside a carefully regulated, controlled environment (Lee et al, 2019). The composition of growth medium is an important factor affecting growth and morphogenesis of plant tissues.…”

Section: Introductionmentioning

confidence: 99%

Effect of Culture Media on Shoot Proliferation and Callus Induction of Bael (Aegle marmelos L.)

Raj¹,

Jakhar²,

Ahmad³

et al. 2020

Int.J.Curr.Microbiol.App.Sci

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A novel automated transplanting system for plant tissue culture

Cited by 10 publications

References 4 publications

Design and Evaluation of a Smart Ex Vitro Acclimatization System for Tissue Culture Plantlets

Design and Evaluation of a Smart Ex Vitro Acclimatization System for Tissue Culture Plantlets

In Vitro Propagation of Date Palm (Phoenix Dactylifera L.) “Lakramat” Date Palms in Aswan Governorate

Effect of Culture Media on Shoot Proliferation and Callus Induction of Bael (Aegle marmelos L.)

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