A novel bacteriocin BM1029: physicochemical characterization, antibacterial modes and application

Abstract

Aim Bacteriocins with antimicrobial activity are considered as potential natural bio‐preservatives to control the growth of food spoilage bacteria. The aim of this work was to characterize a novel bacteriocin BM1029 discovered from Lactobacillus crustorum MN047 and evaluate its antibacterial mechanism. Methods and Results Bacteriocin BM1029 was purified by cation‐exchange chromatography and reversed‐phase chromatography. Antibacterial activity assay showed that BM1029 is antagonistic against both Gram‐positive and Gram‐negative bacteria. Furthermore, it was found that BM1029 showed low haemolysis with high stability to the pretreatment with different temperatures, pH and surfactants. Moreover electron microscopy and flow cytometry suggested that BM1029 inhibit indicator strains by damaging the cell envelope integrity. Cell cycle assay suggested that BM1029 arrested cell cycle in R‐phase. Conclusion The novel bacteriocin BM1029 showed high bactericidal activity against Escherichia coli and Staphylococcus aureus through a cell envelope‐associated mechanism. Significance and Impact of the Study Application of BM1029 inhibited the growth of indicator strains on beef meat storage at 4°C suggesting that this bacteriocin is promising to be used as a novel preservative in food processing and preservation.

Purification and characterization of bacteriocin produced by a strain of Lacticaseibacillus rhamnosus ZFM216

“…Frontiers in Microbiology frontiersin.org its indicator, was detected TEM and SEM (Yan et al, 2021). The influence of bacteriocin (BAC-IB17) on membrane integrity of methicillin-resistant S. aureus was also confirmed by SEM (Ansari et al, 2020).…”

“…These two methods were used by Li et al ( Zhang et al, 2016 ) to infer that the mode-of-action of the two-peptide bacteriocin plnEF on sensitive strains causes cell membrane damage. Bacteriocin BM1029, which can cause pore formation on its indicator, was detected by TEM and SEM ( Yan et al, 2021 ). The influence of bacteriocin (BAC-IB17) on membrane integrity of methicillin-resistant S. aureus was also confirmed by SEM ( Ansari et al, 2020 ).…”

Mining, heterologous expression, purification, antibactericidal mechanism, and application of bacteriocins: A review

“…Obviously, the OD260 values and OD280 values were increased after treatment of enterocin TJUQ1 against L. monocytogenes CMCC 1595 (Qiao et al., 2020). Similarly, the level of ATP and LDH in tested bacteria was also improved when exposed to different kinds of bacteriocins (Lu et al., 2020; Lv et al., 2018; Qiao et al., 2020; Yan et al., 2020). Besides, the K + concentration in the cell supernatants treated with bacteriocins could be determined by flame photometry (Sharma et al., 2020), Inductively coupled plasma mass spectrometry (ICP–MS) (Zhu et al., 2014), and Optima 8000 plasma atomic emission spectrometry (Du et al., 2018a).…”

“…When salivaricin mmaye1 was added to M. luteus , fluorescence intensity of M. luteus stained with SYTOX Green was more than that of untreated cells (Wayah & Philip, 2018), which is similar to plantaricin K25 against B. cereus (Wen et al., 2016). Similarly, the PI intake was improved in indicator bacteria after adding E20c (Sharma et al., 2020), BM1029 (Yan et al., 2020), and plantaricin GZ1‐27 (Du et al., 2018a). Interestingly, the phenomenon of pore formation caused by bacteriocins can be investigated by scanning electron microscopy and transmission electron microscopy in several studies (Qiao et al., 2020; Wen et al., 2016; Yan et al., 2020).…”

“…Similarly, the PI intake was improved in indicator bacteria after adding E20c (Sharma et al., 2020), BM1029 (Yan et al., 2020), and plantaricin GZ1‐27 (Du et al., 2018a). Interestingly, the phenomenon of pore formation caused by bacteriocins can be investigated by scanning electron microscopy and transmission electron microscopy in several studies (Qiao et al., 2020; Wen et al., 2016; Yan et al., 2020).…”