2018
DOI: 10.3389/fmicb.2018.00167
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A Novel Bifunctional Endolytic Alginate Lyase with Variable Alginate-Degrading Modes and Versatile Monosaccharide-Producing Properties

Abstract: Endo-type alginate lyases usually degrade alginate completely into various size-defined unsaturated oligosaccharide products (≥disaccharides), while exoenzymes primarily produce monosaccharide products including saturated mannuronate (M) and guluronate (G) units and particularly unsaturated Δ units. Recently, two bifunctional alginate lyases have been identified as endolytic but M- and G-producing with variable action modes. However, endolytic Δ-producing alginate lyases remain undiscovered. Herein, a new Flam… Show more

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Cited by 49 publications
(27 citation statements)
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“…After storage at 30 • C for 1 h, the activity of TM3 was approximately 57%, much higher than that of TM2. This was similar to the results reported for Aly2 by Peng et al [22]. Notably, after the FL protein had been stored at 60 • C for 1 h, it retained approximately 20% activity and still had 5% activity after incubation at 80 • C for 1 h. We identified an unusual synergy in thermostability between CBM9 and CBM32, which had never been reported previously.…”
Section: Discussionsupporting
confidence: 91%
“…After storage at 30 • C for 1 h, the activity of TM3 was approximately 57%, much higher than that of TM2. This was similar to the results reported for Aly2 by Peng et al [22]. Notably, after the FL protein had been stored at 60 • C for 1 h, it retained approximately 20% activity and still had 5% activity after incubation at 80 • C for 1 h. We identified an unusual synergy in thermostability between CBM9 and CBM32, which had never been reported previously.…”
Section: Discussionsupporting
confidence: 91%
“…A9mT and PyAly contained QVH and were polyM-specific [ 24 , 38 ]. The polyG-specific enzymes, such as Alg2A, ALG-5, and Aly2, all contained QIH sequence [ 26 , 30 , 37 ]. Contrary to this accepted rule, AlyA a was active toward polyM, containing QIH sequence which was similar to FlAlyA and AlyDW11 [ 27 , 31 ].…”
Section: Resultsmentioning
confidence: 99%
“…However, alginate lyase Aly2 encoded by the genome of Flammeovirga sp. strain MY04 showed its maximum activity at pH 6 (Peng et al, 2018 (Inoue et al, 2014).…”
Section: Biochemical Properties and Catalytic Mechanismsmentioning
confidence: 99%