A novel bioassay for the monitoring of hydrogen peroxide in human plasma samples based on binding of horseradish peroxidase-conjugated prostate specific antigen to poly (toluidine blue) as imprinted polymer receptor

Conducting dyes as electro-active monomers and polymers for detecting analytes in biological and environmental samples

“…Additionally, the interaction of horseradish peroxidase-conjugated prostate specific antigen HRP(PSA) with the receptor, poly(toluidine blue), was used for assessing hydrogen peroxide in plasma. Poly(toluidine blue) act as supporting substrate for enhancing the surface area making the rebinding of HRP(PSA) an easy process [ 69 ]. Moreover, toluidine blue-functionalized metal-organic framework films were prepared for the electrochemical immunosensing of indole-3-acetic acid.…”

Enzymatic recognition of hydrogen peroxide (H2O2) in human plasma samples using HRP immobilized on the surface of poly(arginine‐toluidine blue)‐ Fe3O4 nanoparticles modified polydopamine; A novel biosensor

“…Various analytical methods have been reported for the determination of H 2 O 2 such as spectrophoto-and spectrofluorimetric 7,8 chemiluminescence 9,10 and electrochemical methods. 11 Electrochemical biosensor has gained great attention due to high sensitivity, ease of operation portability, low price, and quick response. [11][12][13] Enzyme-catalyzed reactions have been extremely carried out for evaluation of H 2 O 2 .…”

“…11 Electrochemical biosensor has gained great attention due to high sensitivity, ease of operation portability, low price, and quick response. [11][12][13] Enzyme-catalyzed reactions have been extremely carried out for evaluation of H 2 O 2 . This analyte (H 2 O 2 ) as a classic heme enzyme has been extremely utilized for the determination of H 2 O 2 as mimetic enzymes.…”