A novel biomarker for detection of Listeria species in food processing factory

Phraephaisarn, Chirapiphat; Khumthong, Rabuesak; Takahashi, Hiroki; Ohshima, Chihiro; Kodama, Kanako; Techaruvichit, Punnida; Vesaratchavest, Mongkol; Taharnklaew, Rutjawate; Keeratipibul, Suwimon

doi:10.1016/j.foodcont.2016.10.001

Cited by 7 publications

(5 citation statements)

References 20 publications

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“…While 1/2a, 1/2b, 4b are isolated from the clinical cases, 1/2a is mostly isolated from foods (Soni et al, 2013;Barre et al, 2016;Reda et al, 2016). The major risk population groups at risk for invasive listeriosis are the immunocompromised such as pregnant women, unborn or newly delivered infants, organ transplant recipients, cancer and AIDS patients, and the elderly, with fatality rates of 20-30% (Yehia et al, 2016;Phraephaisarn et al, 2017). Different environments such as soil, surface water, sewage, animal feed, farm environments, food processing equipments and environments, urban and suburban settlements are found be contaminated with Listeria spp.…”

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confidence: 99%

Detection of Listeria Spp. In Raw Milk and Dairy Products Retailed in Ankara

Şanlibaba

Tezel

Çakmak³

2018

Gıda

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The objective of this study was to determine the prevalence of Listeria species in raw milk, and dairy products. A total of 110 samples were obtained from randomly selected retail stores and local bazaars located in Ankara. Using TS EN ISO 11290-1 method, 23 samples were found positive for Listeria spp. The overall prevalence of Listeria spp. was 20.91%, in which L. innocua was the most commonly recovered species (6.36%). The remaining isolates were identified as L ivanovii (5.45%), L. monocytogenes (4.55%), and L welshimeri (4.55%). The L monocytogenes isolates were positive for the presence of hlyA gene. The highest prevalence of Listeria spp. was found in homemade cheese (9.09%), followed by raw milk (8.19%), and white cheese (3.64%). L monocytogenes was isolated from raw milk and homemade cheese in this study. In conclusion, the low hygienic quality dairy products may lead to listeriosis surveillance in Ankara.

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confidence: 99%

Detection of Listeria Spp. In Raw Milk and Dairy Products Retailed in Ankara

Şanlibaba

Tezel

Çakmak³

2018

Gıda

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“…Human listeriosis is a serious or even deadly sickness for particular populations, including newborns, pregnant women, the elderly, and individuals with weakened immune systems, with fatality rates for listeriosis ranging from 20 to 30%. It causes sporadic and epidemic outbreaks of the disease (Phraephaisarn et al, 2017;Sarfraz et al, 2017;Şanlıbaba, 2018).…”

Section: Journal Of Animal Health and Productionmentioning

confidence: 99%

Molecular Detection of Listeria Species Isolated From Raw Milk with Special Reference to Virulence Determinants and Antimicrobial Resistance in Listeria monocytogenes

Mohamed¹,

K.G.²,

Al-Azeem³

et al. 2022

JAHP

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Listeria monocytogenes is classified as a major foodborne pathogen associated with high mortalities due to severe invasive listeriosis, meningitis and abortion in humans. With particular emphasis on Listeria monocytogenes' capacity to form biofilms, antimicrobial resistance profile and virulence determinants, the purpose of this study is to discuss the presence of Listeria species in raw milk. In total, 150 samples of raw milk from vendors and nearby dairy farms in Sohag, Egypt, were collected. The samples underwent a bacteriological investigation. The results showed that 22 out of 150 samples had Listeria spp. contamination. Polymerase chain reaction (PCR) using the Listeria iap gene identified 13 of the 22 isolates that were reported as biochemically positive Listeria spp. Eight isolates of Listeria monocytogenes were molecularly confirmed, while the remaining five were subjected to 16S rRNA sequence analysis and identified as L. innocua (three isolates) and L. welshimeri (two isolates). The antibiotic susceptibility profiling revealed multidrug resistance of L. monocytogenes strains against several antimicrobials in addition, they harbored antibiotic resistance genes, including. ampC, aad6, tetM each present in 100% of our isolates and mefA (37.5%). Furthermore, different virulence genes, including the most often found virulence-associated genes hlyA and inlB (100% for each), inlA (50%) were present in our isolates. Interestingly, all of the isolates demonstrated varying degrees of biofilm forming capability. The pathogenicity of L. monocytogenes, particularly virulent, drug-resistant and biofilm-forming strains, is highlighted in this study and can result in a public health danger when present in raw milk. Therefore, it is essential to keep an eye on bacterial resistance in the setting of food production.

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“…Listeria spp. have often been isolated from food and other aspects of the environment, such as soil (5–7). To date, L. newyorkensis strains have been isolated only from food sources (1, 2).…”

Section: Announcementmentioning

confidence: 99%