Whole-Genome Sequence of Listeria newyorkensis, Isolated from River Water in Japan

Ohshima, Chihiro; Takahashi, Hiroki; Nakamura, Ayaka; Kuda, Takashi; Kimura, Bon

doi:10.1128/mra.00488-19

Cited by 3 publications

(3 citation statements)

References 6 publications

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“…Until now, L. newyorkensis was isolated from a milk processing company in the state of New York and from river water in Japan [ 77 , 78 ]. In our study, L. newyorkensis was isolated from swabs (drains) and water samples (a single processing water sample for lettuce and an irrigation water sample for vegetables).…”

Section: Discussionmentioning

confidence: 99%

Genetic Characterization of Listeria from Food of Non-Animal Origin Products and from Producing and Processing Companies in Bavaria, Germany

Wartha¹,

Bretschneider²,

Dangel³

et al. 2023

Foods

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Reported cases of listeriosis from food of non-animal origin (FNAO) are increasing. In order to assess the risk of exposure to Listeria monocytogenes from FNAO, the genetic characterization of the pathogen in FNAO products and in primary production and processing plants needs to be investigated. For this, 123 samples of fresh and frozen soft fruit and 407 samples of 39 plants in Bavaria, Germany that produce and process FNAO were investigated for Listeria contamination. As a result, 64 Listeria spp. isolates were detected using ISO 11290-1:2017. Environmental swabs and water and food samples were investigated. L. seeligeri (36/64, 56.25%) was the most frequently identified species, followed by L. monocytogenes (8/64, 12.50%), L. innocua (8/64, 12.50%), L. ivanovii (6/64, 9.38%), L. newyorkensis (5/64, 7.81%), and L. grayi (1/64, 1.56%). Those isolates were subsequently sequenced by whole-genome sequencing and subjected to pangenome analysis to retrieve data on the genotype, serotype, antimicrobial resistance (AMR), and virulence markers. Eight out of sixty-four Listeria spp. isolates were identified as L. monocytogenes. The serogroup analysis detected that 62.5% of the L. monocytogenes isolates belonged to serogroup IIa (1/2a and 3a) and 37.5% to serogroup IVb (4b, 4d, and 4e). Furthermore, the MLST (multilocus sequence typing) analysis of the eight detected L. monocytogenes isolates identified seven different sequence types (STs) and clonal complexes (CCs), i.e., ST1/CC1, ST2/CC2, ST6/CC6, ST7/CC7, ST21/CC21, ST504/CC475, and ST1413/CC739. The core genome MLST analysis also showed high allelic differences and suggests plant-specific isolates. Regarding the AMR, we detected phenotypic resistance against benzylpenicillin, fosfomycin, and moxifloxacin in all eight L. monocytogenes isolates. Moreover, virulence factors, such as prfA, hly, plcA, plcB, hpt, actA, inlA, inlB, and mpl, were identified in pathogenic and nonpathogenic Listeria species. The significance of L. monocytogenes in FNAO is growing and should receive increasing levels of attention.

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Section: Discussionmentioning

confidence: 99%

Genetic Characterization of Listeria from Food of Non-Animal Origin Products and from Producing and Processing Companies in Bavaria, Germany

Wartha¹,

Bretschneider²,

Dangel³

et al. 2023

Foods

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“…Two sensu lato species, L. newyorkensis and L. fleischmannii , have been isolated from both natural environments and food or food-associated environments in both North America and Europe. L. newyorkensis has been detected in soils in the state of Minnesota, USA ( 20 ), raw milk in Italy ( 47 ), river water in Japan ( 48 ), and in a seafood processing facility in northeastern USA ( 12 ). L. fleischmannii has been isolated from soils in the state of Iowa, USA ( 20 ), cheeses in Italy and Switzerland ( 10 ), and the environment of a cattle ranch in the state of Colorado, USA ( 49 ).…”

Section: Ecology and Distributionmentioning

confidence: 99%

“…Since 2013, 20 new Listeria species have been described (an average of two new species per year), with many of them showing at least some phenotypic characteristics incongruent with their classification within the genus Listeria (e.g., lack of ability to grow at 4°C for at least 8 species and lack of motility for at least 15 species). In 2013, Listeria weihenstephanensis ( 9 ) and Listeria fleischmannii ( 10 ) were described, followed by Listeria floridensis , Listeria aquatica , Listeria cornellensis , Listeria riparia , and Listeria grandensis in 2014 ( 11 ), Listeria booriae and Listeria newyorkensis in 2015 ( 12 ), Listeria costaricensis ( 13 ) and Listeria goaensis ( 14 ) in 2018, Listeria thailandensis in 2019 ( 15 ), Listeria valentina in 2020 ( 16 ), Listeria portnoyi , Listeria rustica , Listeria farberi , Listeria immobilis , and Listeria cossartiae in 2021 ( 17 ), and Listeria ilorinensis ( 18 ) and Listeria swaminathanii ( 19 ) in 2022 ( 20 – 22 ). Here, we provide a review of the phylogenetic, phenotypic, and ecological characteristics of the newly described Listeria s pecies, with a special focus on those described between 2010 and 2022.…”

Section: Introductionmentioning

confidence: 99%

Taxonomy, ecology, and relevance to food safety of the genus Listeria with a particular consideration of new Listeria species described between 2010 and 2022

Orsi,

Liao,

Carlin

et al. 2024

mBio

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Since 2010, the genus Listeria has had the addition of 22 new species that more than tripled the number of species identified until 2010. Sixteen of these 22 new species are distantly related to the type species, Listeria monocytogenes , and several of these present phenotypes that distinguish them from classical Listeria species ( L. monocytogenes , Listeria innocua , Listeria ivanovii, Listeria seeligeri , Listeria welshimeri , and Listeria grayi ). These 22 newly described species also show that Listeria is more genetically diverse than previously estimated. While future studies and surveys are needed to clarify the distribution of these species, at least some of these species may not be widely spread, while other species may be frequently found spread to human-related settings (e.g., farms and processing facilities), and others may be adapted to specific environmental habitats. Here, we review the taxonomic, phylogenetic, and ecological characteristics of these new Listeria species identified since 2010 and re-iterate the suggestion of re-classification of some species into three new genera: Murraya , Mesolisteria , and Paenilisteria . We also provide a review of current detection issues and the relevance to food safety related to the identification of these new species. For example, several new non-pathogenic species could be misidentified as the pathogen L. monocytogenes , based on methods that do not target L. monocytogenes -specific virulence genes/factors, leading to unnecessary product recalls. Moreover, eight species in the proposed new genus Mesolisteria are not good indicators of environmental conditions that could allow L. monocytogenes to grow since Mesolisteria species are unable to grow at low temperatures.

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Listeria monocytogenes: SOROTIPOS E LINHAGENS

Rodrigues,

Chagas

2024

Rev. Foco

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A listeriose é uma doença de origem alimentar que pode ser grave e mortal em grupos de alto risco, que inclui pacientes imunocomprometidos, idosos, neonatos e gestantes. A maioria dos casos de listeriose são esporádicos, mas há relatos surtos ao redor do mundo. Entre as espécies do gênero Listeria, L. monocytogenes é a principal espécie patogênica que afeta tanto animais quanto humanos. L. monocytogenes é caracterizada como uma bactéria Gram-positiva, não formadora de esporos, não encapsulada, móvel, anaeróbica facultativa e intracelular. A transmissão ocorre através da ingestão de alimentos contaminados. L. monocytogenes é uma espécie geneticamente heterogênea dividida em diferentes sorotipos e linhagens filogenéticas. Nesse trabalho, apresentamos uma breve revisão narrativa sobre os sorotipos e linhagens desta espécie bacteriana.

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Whole-Genome Sequence of Listeria newyorkensis, Isolated from River Water in Japan

Abstract: Listeria newyorkensis is a bacterium that was recently classified into the genus Listeria. So far, there are only two reports of isolation of L. newyorkensis from food. Here, we report the whole-genome sequence of L. newyorkensis strain 2-1, isolated from river water in Japan.

Cited by 3 publications

References 6 publications

Genetic Characterization of Listeria from Food of Non-Animal Origin Products and from Producing and Processing Companies in Bavaria, Germany

Genetic Characterization of Listeria from Food of Non-Animal Origin Products and from Producing and Processing Companies in Bavaria, Germany

Taxonomy, ecology, and relevance to food safety of the genus Listeria with a particular consideration of new Listeria species described between 2010 and 2022

Listeria monocytogenes: SOROTIPOS E LINHAGENS

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