A novel bZIP gene from Tamarix hispida mediates physiological responses to salt stress in tobacco plants

Wang, Yucheng; Gao, Caiqiu; Liang, Yenan; Wang, Chao; Yang, Chen; Liu, Guifeng

doi:10.1016/j.jplph.2009.09.008

Cited by 142 publications

(76 citation statements)

References 29 publications

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“…This finding is in agreement with those found in different tolerant plants by many investigators, for example Halimione portulacoides by Kalir et al (1984). Sreenivasulu et al (2000) stated that higher peroxidase isoenzymes were detected in salt tolerant cultivars compared to salt susceptible cultivar of Fox-Tail millet, tolerant cultivars of wheat (Moghaieb et al, 2010), Allium cepa (Abd El-baky et al, 2010) and Tamarix hispida (Wang et al, 2010). Peroxidase playes an important role in the prevention of oxidative damage caused by environmental stress to the membrane lipids (Kalir et al, 1984 andChen et al, 2007).…”

Section: Discussionsupporting

confidence: 90%

Genetical and Physiological Variability Between Tamarix Aphylla and Tamarix Nilotica Species of Oyoun Mousa Region, Sinai, Egypt

Mekki

El-Meleigy

2013

Egyptian Journal of Desert Research

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amarix aphylla trees occupy a distinct zone along the sand dune in Oyoun Mousa region. Genetical and physiological studies on the oldest tree T. aphylla and the T. nilotica species in the same region were carried out. Electrophoretic analysis of total soluble protein (SDS-PAGE) and some isoenzymes; such as acid phosphatase (ACPH), esterase (EST), peroxidase (POD) and 6-phosphogluconate dehydrogenase (6PGD) revealed that T. aphylla exhibited higher number of polypeptides and isoformes. This finding might suggest that T. aphylla plants are more tolerant to salt stress than T. nilotica. This could be accomplished by increasing the capacity of antioxidative system, synthesis of new protein and isoenzymes, which could in turn contribute to some defense mechanisms of tolerant plant. Random Amplified Polymorphic (RAPD) DNA markers were used to measure genetic diversity of the two species. A total of 50 amplified bands were scored with the used of 8 RAPD primers, with a mean of 6.2 amplified bands per primer, and 66% (33 bands) of polymorphic bands were found. The use of DNA markers, OPA-7, OPA-10, OPA-15 and OPB-4 distinguished bands with size ranging from 1655 to 2500 bp in T. aphylla only and absent in T. nilotica.

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Section: Discussionsupporting

confidence: 90%

Genetical and Physiological Variability Between Tamarix Aphylla and Tamarix Nilotica Species of Oyoun Mousa Region, Sinai, Egypt

Mekki

El-Meleigy

2013

Egyptian Journal of Desert Research

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“…MDA is one of the final products of polyunsaturated fatty acid peroxidation in plants, and an increase in free radicals leads to overproduction of MDA (Gaweł et al 2004). Therefore, MDA levels are related to the extent of damage to the ultrastructure (Sairam et al 2002), and MDA levels during stress conditions is well-known to be an important marker for evaluating the stress tolerance of plants (Wang et al 2009b). Another result of lipid peroxidation is the changes of intact microsomal membranes (Senaratna et al 1985), resulting in larger membranes porosity and increased permeability (Mukherjee and Choudhuri 1983).…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, it is important for plants to enhance their ROS-scavenging capacity to adapt to low temperature stress conditions. POD and SOD are important ROS-scavenging enzymes in plants (Wang et al 2009b). Thus, by measuring the activities of POD and SOD we can indirectly assess the ability of plants to tolerate low temperatures.…”

Section: Discussionmentioning

confidence: 99%

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Improvement of cold tolerance of the half-high bush Northland blueberry by transformation with the LEA gene from Tamarix androssowii

2010

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Previous studies have shown that the late embryogenesis abundant (LEA) gene of Tamarix androssowii can enhance the drought tolerance of transgenic tobacco. In this study, the cloned LEA gene was transformed into half-high bush Northland blueberry in order to enhance its ability to tolerate cold stress. The cephalosporin antibiotics ceftriaxone, cefotaxime and cefazolin were used to optimize transformation of leaf explants, and kanamycin sulfate was used to select for transgenic shoots. PCR and Southern blot analysis confirmed 8 transformants with LEA gene copy numbers ranging from 1 to 7. The LEA chimeric gene was found to be normally transcribed in 6 transgenic lines by RT-PCR. The 8 transgenic lines were tested for cold tolerance by measuring the activities of peroxidase (POD) and superoxide dismutase (SOD), malondialdehyde (MDA) content and relative electrolyte leakage (REL). Overexpression of the LEA gene enhanced the activity of both POD and SOD under low temperature stress conditions. Lipid peroxidation in the transgenic lines was significantly lower than in non-transgenic plants after cold stress, as determined by MDA content and REL. Thus, our findings indicate that the LEA gene confers increased cold tolerance in the Northland blueberry and implicate the metabolic pathways through which it exerts this effect.

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“…Most studies have shown that bZIP transcription factors play an important role in plants responsive to adverse stresses (Després et al 2000(Després et al , 2003Fan and Dong 2002;Jakoby et al 2002;Kim et al 2003;Lee et al 2006b;Liao et al 2008aLiao et al , 2008bNijhawan et al 2008). Plants overexpressing exogenous bZIP genes show an obvious tolerance to water deficit/drought stress and heat shock , freezing (Liao et al 2008b), salt stress (Liao et al 2008b;Wang et al 2010b), and methyl viologen-oxidative stress (Lee et al 2006b). bZIP proteins could increase disease resistance (Fan and Dong 2002;Lee et al 2006b) and enhance the expression of pathogenesis-related genes (PRs) for systemic acquired resistance (SAR) in plants (Lee et al 2006a).…”

Section: Introductionmentioning

confidence: 99%

Overexpression of the Malus hupehensis MhTGA2 Gene, a Novel bZIP Transcription Factor for Increased Tolerance to Salt and Osmotic Stress in Transgenic Tobacco

Zhang

et al. 2012

International Journal of Plant Sciences

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Basic leucine zipper (bZIP) proteins form one of the largest families of transcription factors, and their individual contributions in a particular regulatory network remain difficult to assess. Here, we identified and functionally characterized the Malus hupehensis bZIP transcription factor designated MhTGA2 from M. hupehensis leaves treated with salicylic acid (SA). The MhTGA2 protein was localized in the nucleus. The expression of MhTGA2 was higher in leaves than in stems and roots. This gene was significantly induced by SA, methyl jasmonate, and 1-aminocyclopropane-1-carboxylic acid and weakly induced by abscisic acid. The gene was clearly induced by low temperature (4°C) and NaCl and weakly induced by polyethylene glycol but not induced by apple ring spot pathogens. Overexpression of the MhTGA2 gene in transgenic tobacco plants conferred enhanced resistance to NaCl at the stage of seed germination and in seedlings, and it conferred resistance to mannitol at the stage of seed germination. Furthermore, overexpression of MhTGA2 led to higher expression levels of pathogenesis-related proteins and osmotic-stress-related genes in transgenic tobacco than in wild-type plants. These results suggest that the MhTGA2 transcription factor might be active in systemic acquired resistance and might function as a possible regulator of enhanced environmental-stress tolerance. MhTGA2 is thus a potentially constructive candidate gene for engineering salt and osmotic stress tolerance in cultivated plants.

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A novel bZIP gene from Tamarix hispida mediates physiological responses to salt stress in tobacco plants

Cited by 142 publications

References 29 publications

Genetical and Physiological Variability Between Tamarix Aphylla and Tamarix Nilotica Species of Oyoun Mousa Region, Sinai, Egypt

Genetical and Physiological Variability Between Tamarix Aphylla and Tamarix Nilotica Species of Oyoun Mousa Region, Sinai, Egypt

Improvement of cold tolerance of the half-high bush Northland blueberry by transformation with the LEA gene from Tamarix androssowii

Overexpression of the Malus hupehensis MhTGA2 Gene, a Novel bZIP Transcription Factor for Increased Tolerance to Salt and Osmotic Stress in Transgenic Tobacco

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