A novel chemotactic factor derived from the extracellular matrix protein decorin recruits mesenchymal stromal cells in vitro and in vivo

Dempsey, Sandi G.; Miller, Christopher H.; Schueler, Julia; Veale, Robert W. F.; Day, Darren J.

doi:10.1371/journal.pone.0235784

Cited by 15 publications

(15 citation statements)

References 76 publications

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“…9 This structural and compositional mimicry of healthy tissue ECM facilitates a range of biological properties, for example OFM to inhibit a range of tissue proteases, 21 and stimulate cell migration, infiltration, proliferation and differentiation, 7,8,22 and the recruitment of mesenchymal stromal cells. 10 In comparison to a synthetic dermal matrix, such as reconstituted collagen/ glycosaminoglycan, the OFM graft is expected to impart greater biological functionality, attributed to a preserved ECM structure and composition.…”

Section: Discussionmentioning

confidence: 99%

“…Ovine forestomach matrix (OFM) is a decellularised xenograft ECM biomaterial composed of over 150 matrisome proteins, 6 and an open porous architecture to support cell infiltration. [7][8][9][10] OFM is manufactured from ovine forestomach using processes to remove the ovine cells, while retaining the architecture and composition of the tissue ECM. Clinically, OFM-based devices have been used in hard-to-heal wound management, plastics and general surgery.…”

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confidence: 99%

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Extracellular matrix graft for reconstruction over exposed structures: a pilot case series

Bohn

Chaffin

2020

J Wound Care

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Objective: Soft tissue defects, especially those involving exposed vital structures, present a reconstructive challenge because poor vascularity of such defects typically makes immediate skin grafting unviable. Where flap procedures are inappropriate or not possible, dermal matrices represent an alternative reconstructive option for defects with denuded vital structures. With dermal matrices becoming increasingly available and technologically advanced, we evaluated an ovine-derived extracellular matrix graft in the reconstruction of complex soft tissue defects involving exposed vital structures. Method: Six cases of soft tissue defects exhibiting denuded vital structures underwent reconstruction using an ovine forestomach matrix graft as a dermal matrix. Grafts were fixed directly into defects for immediate coverage and subsequently temporised defects via granulation tissue formation for later skin graft or secondary closure. Defect granulation and epithelialisation were monitored until closure and the final aesthetic and functional outcomes were evaluated. Results: Complete healing was achieved in all cases, with defect granulation becoming observable within one to two weeks and complete granulation occurring within one to six weeks. Granulation tissue resulting from the graft was suitable for skin grafting, with 100% take of skin grafts after one week and complete re-epithelialisation in two to three weeks in the four cases that received a skin graft. Good cosmetic, functional and patient satisfaction outcomes were achieved in all cases. Conclusion: The present series demonstrates our initial use of an extracellular matrix-based dermal matrix in reconstructing defects with exposed vital structures. While such dermal matrices do not supersede or replace flap procedures, they represent an alternative option on the reconstructive ladder in cases where flap procedures are not appropriate or possible.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Extracellular matrix graft for reconstruction over exposed structures: a pilot case series

Bohn

Chaffin

2020

J Wound Care

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“…Decorin ( Figure 3E ) regulates cellular survival, migratory, proliferative and angiogenic signaling and collagen fibril formation, sequesters TGF-β and antagonizes receptor tyrosine kinase family members, including EGFR and IGF-IR ( Schönherr et al, 2005 ; Iozzo et al, 2011 ; Neill et al, 2012 ). MayDay, a ∼12 kDa N-terminal chemotactic factor, generated by macrophage-induced MMP-12 cleavage of decorin, recruits mesenchymal stem cells (MSCs) to damaged tissue regions in vitro and in vivo , promoting tissue repair ( Dempsey et al, 2020 ). In situ hybridization (ISH) has localized decorin in areas of microvascular proliferation within gliomas and may be a therapeutic target in anti-angiogenic therapy ( Patel et al, 2020 ) or approaches targeting TGF-β activity in tumors ( Birch et al, 2020 ).…”

Section: The Small Neural Proteoglycansmentioning

confidence: 99%

Neural Tissue Homeostasis and Repair Is Regulated via CS and DS Proteoglycan Motifs

Hayes

Melrose

2021

Front. Cell Dev. Biol.

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Chondroitin sulfate (CS) is the most abundant and widely distributed glycosaminoglycan (GAG) in the human body. As a component of proteoglycans (PGs) it has numerous roles in matrix stabilization and cellular regulation. This chapter highlights the roles of CS and CS-PGs in the central and peripheral nervous systems (CNS/PNS). CS has specific cell regulatory roles that control tissue function and homeostasis. The CNS/PNS contains a diverse range of CS-PGs which direct the development of embryonic neural axonal networks, and the responses of neural cell populations in mature tissues to traumatic injury. Following brain trauma and spinal cord injury, a stabilizing CS-PG-rich scar tissue is laid down at the defect site to protect neural tissues, which are amongst the softest tissues of the human body. Unfortunately, the CS concentrated in gliotic scars also inhibits neural outgrowth and functional recovery. CS has well known inhibitory properties over neural behavior, and animal models of CNS/PNS injury have demonstrated that selective degradation of CS using chondroitinase improves neuronal functional recovery. CS-PGs are present diffusely in the CNS but also form denser regions of extracellular matrix termed perineuronal nets which surround neurons. Hyaluronan is immobilized in hyalectan CS-PG aggregates in these perineural structures, which provide neural protection, synapse, and neural plasticity, and have roles in memory and cognitive learning. Despite the generally inhibitory cues delivered by CS-A and CS-C, some CS-PGs containing highly charged CS disaccharides (CS-D, CS-E) or dermatan sulfate (DS) disaccharides that promote neural outgrowth and functional recovery. CS/DS thus has varied cell regulatory properties and structural ECM supportive roles in the CNS/PNS depending on the glycoform present and its location in tissue niches and specific cellular contexts. Studies on the fruit fly, Drosophila melanogaster and the nematode Caenorhabditis elegans have provided insightful information on neural interconnectivity and the role of the ECM and its PGs in neural development and in tissue morphogenesis in a whole organism environment.

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“…5 Ovine forestomach matrix retains the native collagen structure seen in normal tissue ECM, 6 as well as functional secondary molecules required for cell adhesion, migration, proliferation and rapid development of capillary networks. 5,[7][8][9] OFM has been used as 1 Department of Research and Clinical Development, Aroa Biosurgery Limited, Auckland, New Zealand 2 a universal building block for the design of devices for soft tissue repair, either as single layers of dECM (∼200 μm thick), powders or multi-layered devices (up to ∼3 mm thick) 10,11 ; and as a platform for further functionalization such as antimicrobial dECM devices. 12 OFM-based devices have been adopted into regular clinical practice for the treatment of acute and chronic wounds, [13][14][15][16][17] reconstructive surgery [18][19][20][21] and abdominal wall repair.…”

Section: Introductionmentioning

confidence: 99%

Further structural characterization of ovine forestomach matrix and multi-layered extracellular matrix composites for soft tissue repair

Smith¹,

Dempsey²,

Veale³

et al. 2021

J Biomater Appl

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Decellularized extracellular matrix (dECM)–based biomaterials are of great clinical utility in soft tissue repair applications due to their regenerative properties. Multi-layered dECM devices have been developed for clinical indications where additional thickness and biomechanical performance are required. However, traditional approaches to the fabrication of multi-layered dECM devices introduce additional laminating materials or chemical modifications of the dECM that may impair the biological functionality of the material. Using an established dECM biomaterial, ovine forestomach matrix, a novel method for the fabrication of multi-layered dECM constructs has been developed, where layers are bonded via a physical interlocking process without the need for additional bonding materials or detrimental chemical modification of the dECM. The versatility of the interlocking process has been demonstrated by incorporating a layer of hyaluronic acid to create a composite material with additional biological functionality. Interlocked composite devices including hyaluronic acid showed improved in vitro bioactivity and moisture retention properties.

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A novel chemotactic factor derived from the extracellular matrix protein decorin recruits mesenchymal stromal cells in vitro and in vivo

Cited by 15 publications

References 76 publications

Extracellular matrix graft for reconstruction over exposed structures: a pilot case series

Extracellular matrix graft for reconstruction over exposed structures: a pilot case series

Neural Tissue Homeostasis and Repair Is Regulated via CS and DS Proteoglycan Motifs

Further structural characterization of ovine forestomach matrix and multi-layered extracellular matrix composites for soft tissue repair

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