A Novel Chronoimpedimetric Glucose Sensor in Real Blood Samples Modified by Glucose‐imprinted Pyrrole‐Aminophenylboronic Acid Modified Screen Printed Electrode

Abstract

In this study, a molecularly imprinted sensor technology is engineered to detect glucose in real blood samples by chronoimpedimetrically. The imprinting process of glucose (Glc) was carried out by electrochemical polymerization of aminophenylboronic acid (APBA) and pyrrole (Py) by performing cyclic voltammetry (CV). Afterwards, glucose molecule was removed from imprinted surface by 5 % acetic acid to reveal glucose imprinted cavities. Electrochemical Impedance Spectroscopy (EIS) was used to characterize sensor modification steps and glucose removal. Glucose monitoring process was carried out chronoimpedimetrically(CI) for the first time in real blood samples. Calibration curve was prepared between 20–800 mg/dL. The standard deviations of the 18 calibration curves R2 were calculated as 0.9866±0.0066 to assess reproducibility. Recovery was calculated by using 105 mg/dL Glc Serum Sample, which was monitored by auto analyzer and into this sample 50 mg/dL Glc added and our sensor response was 147.92±2.43 mg/dL, 98.6±1.62 % (n=5). Non‐imprinted (NIP) sensor gave no signal for the glucose concentration.

Functional nanostructure-loaded three-dimensional graphene foam as a non-enzymatic electrochemical sensor for reagentless glucose detection

“…† The LOD is lower than those obtained using amino-PBA functionalized polymer, 44 or MPBA-decorated gold nanoparticle-chitosan modied electrode 45 or boronic acid-modied hydrogel coated quartz disc. 46 The initial concentration of linear detection range of our sensor is lower than that of conductive polymer-decorated CuCo 2 O 4 carbon nanober modied electrode, 47 or glucose-imprinted pyrrole amino-PBA modied electrode, 48 pyrene-1-boronic acid modied graphene, 49 or MPBA monolayer on Au coated optical ber. 50 The LOD is higher than that obtained using amino-PBAfunctionalized graphitic carbon nitride quantum dots, 51 or boronic acid-functionalized hierarchically porous metalorganic frameworks.…”

A Novel Chronoimpedimetric Cytosine Modified Molecularly Imprinted Sensor for Label-Free Detection of 8-Hydroxydeoxyguanosine in Urine Samples

“…This polymerization was carried out at pH = 7 in phosphate buffer containing APBA (40 mM), PyCOOH (60 mM), and 200 mM NaF. The polymerization was performed with CV at a scanning rate of 50 mV s −1 in the potential range between −0.2 and +1.1 V. 19 The same procedure was prepared as a non-imprinted sensor using a mixture without 8OHG (Fig. 1).…”

Review—Trends on the Development of Non-Enzymatic Electrochemical Sensors Modified with Molecularly Imprinted Polymers for the Quantification of Glucose

“…On the other hand, amino phenylboronic acid (APBA) and pyrrole (Py) are conductive polymers with excellent chemical and electrochemical stability properties, for this reason, they have been used in the development of non-enzymatic sensors. 80,81 Onur et al, 82 used a solution of glucose (template molecule), 3aminophenyboronic acid and pyrrole for MIP's synthesis to modify the surface of platinum screen-printed electrodes (Pt-SPE) via electro-polymerization by CV with a wide potential range (−200 mV to 1200 mV), slow scan rate (10 mVs −1 ), 40 cycles (in pH = 7.0 phosphate buffer solution). Subsequently, the template molecule was removed from the electrode with pure water.…”