A novel class of gene controlling virulence in plant pathogenic ascomycete fungi

Lu, Shunwen; Kroken, Scott; Lee, Bee-Na; Robbertse, Barbara; Churchill, Alice C. L.; Yoder, O. C.; Turgeon, B. Gillian

doi:10.1073/pnas.0931375100

Cited by 79 publications

(50 citation statements)

References 41 publications

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“…It is therefore a prime target for biotechnological approaches to combat the fungus. Other genes required for full virulence have been reported (42)(43)(44). Yet mutations in mitogen-activated protein kinases are pleiotropic, affecting not only virulence but also conidiation, perithecia formation, vegetative growth, and mycotoxin production.…”

Section: Glucosylation Of 4-deoxynivalenol Greatly Reduces Itsmentioning

confidence: 99%

“…Yet mutations in mitogen-activated protein kinases are pleiotropic, affecting not only virulence but also conidiation, perithecia formation, vegetative growth, and mycotoxin production. In the case of the recently identified CPS1 gene (44), it is unclear whether the hypothetical adenylate-forming enzyme encoded by the gene is involved in biosynthesis of an unidentified metabolite or necessary for some unknown aspect of stress tolerance, in particular in plants mounting a defense response.…”

Section: Glucosylation Of 4-deoxynivalenol Greatly Reduces Itsmentioning

confidence: 99%

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Detoxification of the Fusarium Mycotoxin Deoxynivalenol by a UDP-glucosyltransferase from Arabidopsis thaliana

Poppenberger

Berthiller²,

Lucyshyn

et al. 2003

Journal of Biological Chemistry

487

414

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Plant pathogenic fungi of the genus Fusarium cause agriculturally important diseases of small grain cereals and maize. Trichothecenes are a class of mycotoxins produced by different Fusarium species that inhibit eukaryotic protein biosynthesis and presumably interfere with the expression of genes induced during the defense response of the plants. One of its members, deoxynivalenol, most likely acts as a virulence factor during fungal pathogenesis and frequently accumulates in grain to levels posing a threat to human and animal health. We report the isolation and characterization of a gene from Arabidopsis thaliana encoding a UDP-glycosyltransferase that is able to detoxify deoxynivalenol. The enzyme, previously assigned the identifier UGT73C5, catalyzes the transfer of glucose from UDP-glucose to the hydroxyl group at carbon 3 of deoxynivalenol. Using a wheat germ extract-coupled transcription/translation system we have shown that this enzymatic reaction inactivates the mycotoxin. This deoxynivalenol-glucosyltransferase (DOGT1) was also found to detoxify the acetylated derivative 15-acetyl-deoxynivalenol, whereas no protective activity was observed against the structurally similar nivalenol. Expression of the glucosyltransferase is developmentally regulated and induced by deoxynivalenol as well as salicylic acid, ethylene, and jasmonic acid. Constitutive overexpression in Arabidopsis leads to enhanced tolerance against deoxynivalenol.

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Section: Glucosylation Of 4-deoxynivalenol Greatly Reduces Itsmentioning

confidence: 99%

Section: Glucosylation Of 4-deoxynivalenol Greatly Reduces Itsmentioning

confidence: 99%

Detoxification of the Fusarium Mycotoxin Deoxynivalenol by a UDP-glucosyltransferase from Arabidopsis thaliana

Poppenberger

Berthiller²,

Lucyshyn

et al. 2003

Journal of Biological Chemistry

487

414

View full text Add to dashboard Cite

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“…Mutations in NPS6 did not cause complete loss of virulence, indicating that additional gene products are required for full virulence. One such factor is CPS1, the product of a gene encoding an acyl-AMP ligase-like enzyme that is required for full virulence by C. heterostrophus, and like NPS6, is ubiquitous among euascomycetes (27). Deletion of CPS1 reduces but does not eliminate virulence on maize; the degree of reduction is less than that of nps6 mutants.…”

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confidence: 99%

Functional Analysis of All Nonribosomal Peptide Synthetases in Cochliobolus heterostrophus Reveals a Factor, NPS6, Involved in Virulence and Resistance to Oxidative Stress

et al. 2005

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Nonribosomal peptides, made by nonribosomal peptide synthetases, have diverse biological activities, including roles as fungal virulence effectors. Inspection of the genome of Cochliobolus heterostrophus, a fungal pathogen of maize and a member of a genus noted for secondary metabolite production, revealed eight multimodular nonribosomal peptide synthase (NPS) genes and three monomodular NPS-like genes, one of which encodes a nonribosomal peptide synthetase/polyketide synthase hybrid enzyme presumed to be involved in synthesis of a peptide/polyketide molecule. Deletion of each NPS gene and phenotypic analyses showed that the product of only one of these genes, NPS6, is required for normal virulence on maize. NPS6 is also required for resistance to hydrogen peroxide, suggesting it may protect the fungus from oxidative stress. This and all other nps mutants had normal growth, mating ability, and appressoria. Real-time PCR analysis showed that expression of all NPS genes is low (relative to that of actin), that all (except possibly NPS2) are expressed during vegetative growth, and that expression is induced by nitrogen starvation. Only NPS6 is unfailingly conserved among euascomycete fungi, including plant and human pathogens and saprobes, suggesting the possibility that NPS6 activity provides oxidative stress protection during both saprobic and parasitic growth.

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“…55 (1), 2005 69 Desjardins et al (1996) 20) Hou et al (2002) 31) Jenczmionka et al (2003) 32) 33,34) 33,34) Lu et al (2003) …”

Section: Whole Genome Sequencingunclassified

Genomic analyses and their applications in Fusarium graminearum

Suga¹

2005

Mycotoxins

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A novel class of gene controlling virulence in plant pathogenic ascomycete fungi

Cited by 79 publications

References 41 publications

Detoxification of the Fusarium Mycotoxin Deoxynivalenol by a UDP-glucosyltransferase from Arabidopsis thaliana

Detoxification of the Fusarium Mycotoxin Deoxynivalenol by a UDP-glucosyltransferase from Arabidopsis thaliana

Functional Analysis of All Nonribosomal Peptide Synthetases in Cochliobolus heterostrophus Reveals a Factor, NPS6, Involved in Virulence and Resistance to Oxidative Stress

Genomic analyses and their applications in Fusarium graminearum

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