A novel clean-up method for urine analysis of low-molecular mass aldehydes by capillary electrophoresis with laser-induced fluorescence detection

Baños, Clara Eugenia; Silva, Manuel

doi:10.1016/j.jchromb.2010.10.033

Cited by 13 publications

(3 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[8] A wide variety of aldehydes are ubiquitous within biogenic fluids (i.e. produced by living organisms), including exhaled human breath, [9][10][11][12] the air adjacent to skin, [13,14] the humid headspace air above urine, [15][16][17] faeces, [1,18,19] blood and mammalian and bacterial cultures. [20][21][22][23] Their presence in breath has been connected with a wide range of diseases, especially cancer, [10,12,24] but no aldehyde has been definitely confirmed as a biomarker of a specific cancer either by detailed laboratory studies or by clinical trials.…”

mentioning

confidence: 99%

Reactions of the selected ion flow tube mass spectrometry reagent ions H₃O⁺and NO⁺with a series of volatile aldehydes of biogenic significance

Smith

Chippendale

Španěl

2014

Rapid Commun. Mass Spectrom.

View full text Add to dashboard Cite

show abstract

mentioning

confidence: 99%

Reactions of the selected ion flow tube mass spectrometry reagent ions H₃O⁺and NO⁺with a series of volatile aldehydes of biogenic significance

Smith

Chippendale

Španěl

2014

Rapid Commun. Mass Spectrom.

View full text Add to dashboard Cite

show abstract

“…For example, the spectrum of PT with 0.4% urine concentration almost overlaps with the spectrum of PT, efficiently masking the fluorescent effect of urine. However, at these low concentrations, the assay sensitivity may be significantly compromised, as the target analyte concentration in the test samples will be reduced proportionally at these high dilutions …”

Section: Results and Discussionmentioning

confidence: 99%

Colorimetric Urinalysis for On-Site Detection of Metabolic Biomarkers

Yeasmin

Ammanath

Ali

et al. 2020

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

Over the past few decades, colorimetric assays have been developed for cost-effective and rapid on-site urinalysis. Most of these assays were employed for detection of biomarkers such as glucose, uric acid, ions, and albumin that are abundant in urine at micromolar to millimolar levels. In contrast, direct assaying of urinary biomarkers such as glycated proteins, low-molecular-weight reactive oxygen species, and nucleic acids that are present at significantly lower levels (nanomolar to picomolar) remain challenging due to the interferences from the urine sample matrix. State-of-the-art assays for detection of trace amounts of urinary biomarkers typically utilize time-consuming and equipment-dependent sample pretreatment or clean-up protocols prior to assaying, which limits their applicability for on-site analysis. Herein, we report a colorimetric assay for on-site detection of trace amount of generic biomarkers in urine without involving tedious sample pretreatment protocols. The detection strategy is based on monitoring the changes in optical properties of poly(3-(4-methyl-3′-thienyloxy)propyltriethylammonium bromide) upon interacting with an aptamer or a peptide nucleic acid in the presence and absence of target biomarkers of relevance for the diagnosis of metabolic complications and diabetes. As a proof of concept, this study demonstrates facile assaying of advanced glycation end products, 8-hydroxy-2′-deoxyguanosine and hepatitis B virus DNA in urine samples at clinically relevant concentrations, with limits of detection of ∼850 pM, ∼650 pM, and ∼ 1 nM, respectively. These analytes represent three distinct classes of biomarkers: (i) glycated proteins, (ii) low-molecular-weight reactive oxygen species, and (iii) nucleic acids. Hence, the proposed methodology is applicable for rapid detection of generic biomarkers in urine, without involving sophisticated equipment and skilled personnel, thereby enabling on-site urinalysis. At the end of the contribution, we discuss the opportunity to translate the homogeneous assay into a paper-based format.

show abstract

“…Prior to spiking with formaldehyde, 6 mL aliquot of the urine sample was subjected to centrifugal filtration using Vivaspin-6 cartridges at a speed of 4000 rpm for 30 min at room temperature. 25 Filtered urine samples were diluted 10-times in PBS. Various concentrations of formaldehyde were spiked in the 10-times diluted urine and were used for analysis.…”

Section: Methodsmentioning

confidence: 99%

Pyrenyl carbon nanostructures for ultrasensitive measurements of formaldehyde in urine

Premaratne

Farias

Krishnan

2017

Analytica Chimica Acta

View full text Add to dashboard Cite

Measurement of ultra-low (e.g., parts-per-billion) levels of small-molecule markers in body fluids (e.g., serum, urine, saliva) involves a considerable challenge in view of designing assay strategies with sensitivity and selectivity. Herein we report for the first time an amperometric nano-bioelectrode design that uniquely combines 1-pyrenebutyric acid units pi-pi stacked with carboxylated multiwalled carbon nanotubes on the surface of gold screen printed electrodes for covalent attachment of NAD+ dependent formaldehyde dehydrogenase (FDH). The designed enzyme bioelectrode offered 6 ppb formaldehyde detection in 10-times diluted urine with a wide dynamic range of 10 ppb to 10 ppm. Fourier transform infrared, Raman, and electrochemical impedance spectroscopic characterizations confirmed the successful design of the FDH bioelectrode. Flow injection analysis provided lower detection limit and greater affinity for formaldehyde (apparent KM 9.6 ± 1.2 ppm) when compared with stirred solution method (apparent KM 19.9 ± 4.6 ppm). Selectivity assays revealed that the bioelectrode was selective toward formaldehyde with a moderate cross-reactivity for acetaldehyde (~ 25%) and negligible cross-reactivity toward propanaldehyde, acetone, methanol, and ethanol. Formaldehyde is an indoor pollutant, and studies have indicated neurotoxic characteristics and systemic toxic effects of this compound upon chronic and high doses of exposure. Moreover, reported chromatography and mass spectrometry methods identified elevated urine formaldehyde levels in patients with bladder cancer, dementia, and early stages of cognitive impairments compared to healthy people. Results demonstrate that pyrenyl carbon nanostructures-based FDH bioelectrode design represents novelty and simplicity for enzyme-selective electrochemical quantitation of small 30 Da formaldehyde. Broader applicability of the presented approach for other small-molecule markers is feasible that requires only the design of appropriate marker-specific enzyme systems or receptor molecules.

show abstract

A novel clean-up method for urine analysis of low-molecular mass aldehydes by capillary electrophoresis with laser-induced fluorescence detection

Cited by 13 publications

References 39 publications

Reactions of the selected ion flow tube mass spectrometry reagent ions H₃O⁺and NO⁺with a series of volatile aldehydes of biogenic significance

Reactions of the selected ion flow tube mass spectrometry reagent ions H₃O⁺and NO⁺with a series of volatile aldehydes of biogenic significance

Colorimetric Urinalysis for On-Site Detection of Metabolic Biomarkers

Pyrenyl carbon nanostructures for ultrasensitive measurements of formaldehyde in urine

Contact Info

Product

Resources

About

A novel clean-up method for urine analysis of low-molecular mass aldehydes by capillary electrophoresis with laser-induced fluorescence detection

Cited by 13 publications

References 39 publications

Reactions of the selected ion flow tube mass spectrometry reagent ions H3O+and NO+with a series of volatile aldehydes of biogenic significance

Reactions of the selected ion flow tube mass spectrometry reagent ions H3O+and NO+with a series of volatile aldehydes of biogenic significance

Colorimetric Urinalysis for On-Site Detection of Metabolic Biomarkers

Pyrenyl carbon nanostructures for ultrasensitive measurements of formaldehyde in urine

Contact Info

Product

Resources

About

Reactions of the selected ion flow tube mass spectrometry reagent ions H₃O⁺and NO⁺with a series of volatile aldehydes of biogenic significance

Reactions of the selected ion flow tube mass spectrometry reagent ions H₃O⁺and NO⁺with a series of volatile aldehydes of biogenic significance