A novel collagen-binding peptide promotes osteogenic differentiation via Ca2+/calmodulin-dependent protein kinase II/ERK/AP-1 signaling pathway in human bone marrow-derived mesenchymal stem cells

Shin, Min Kyoung; Kim, Mi Kyoung; Bae, Yoe Sik; Jo, Inho; Lee, Seung Jin; Chung, Chong Pyoung; Park, Yoon Jeong; Min, Do Sik

doi:10.1016/j.cellsig.2007.11.012

Cited by 64 publications

(49 citation statements)

References 39 publications

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“…differentiation: Cbfa1/Runx2 and AP-1 Cbfa1/Runx2 and AP-1 are important transcription factors involved in osteogenic differentiation of hMSCs (Bjerre et al 2008;Haasper et al 2008;Shin et al 2008). Runx2 is essential not only in the osteogenic differentiation of hMSCs but also in the differentiation of osteoblasts and chondroblasts (Fu et al 2007).…”

Section: Transcription Factors Related To Osteogenicmentioning

confidence: 97%

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Mechanisms for osteogenic differentiation of human mesenchymal stem cells induced by fluid shear stress

Liu

Yuan

Wang

2010

Biomech Model Mechanobiol

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Mechanical stimuli can improve bone function by promoting the proliferation and differentiation of bone cells and osteoblasts. As precursors of osteoblasts, human mesenchymal stem cells (hMSCs) are sensitive to mechanical stimuli. In recent years, fluid shear stress (FSS) has been widely used as a method of mechanical stimulation in bone tissue engineering to induce the osteogenic differentiation of hMSCs. However, the mechanism of this differentiation is not completely clear. Several signaling pathways are involved in the mechanotransduction of hMSCs responding to FSS, such as MAPK, NO/cGMP/PKG and Ca(2+) signaling pathway. Here, we briefly review how hMSCs respond to fluid flow stimuli and focus on the signal molecules involved in this mechanotransduction.

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Section: Transcription Factors Related To Osteogenicmentioning

confidence: 97%

“…The increasing of Ca 2+ leads to the activation of CaMKII, which subsequently phosphorylates ERK1/2 ( Fig. 1) (Shin et al 2008). Therefore, Ca 2+ signal can lead to the activation of ERK1/2 signaling pathway, which is critical for osteoblastic differentiation.…”

Section: No and Pge2mentioning

confidence: 99%

Mechanisms for osteogenic differentiation of human mesenchymal stem cells induced by fluid shear stress

Liu

Yuan

Wang

2010

Biomech Model Mechanobiol

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“…CaMKII is expressed most abundantly in neurons, and is involved in regulating many aspects of neuronal function, including neurotransmitter synthesis and release, modulation of ion channel activity, cellular transport, cellular morphology and neurite extension, long-term plasticity, learning, memory consolidation, and memory erasure following retrieval [2][3][4][5][6][7]. Non-neuronal CaMKII has been implicated in the regulation of other biological processes, such as fertilisation [8], osteogenic differentiation [9], and the maintenance of vascular tone [10]. Aside from its abundance in the brain, intense interest in CaMKII arose from its ability to act as autophosphorylatable molecular switch (reviewed in [11,12]).…”

Section: Introductionmentioning

confidence: 99%

Regulation of CaMKII In vivo: The Importance of Targeting and the Intracellular Microenvironment

Skelding

Rostas

2009

Neurochem Res

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CaMKII (calcium/calmodulin-stimulated protein kinase II) is a multifunctional protein kinase that regulates normal neuronal function. CaMKII is regulated by multi-site phosphorylation, which can alter enzyme activity, and targeting to cellular microdomains through interactions with binding proteins. These proteins integrate CaMKII into multiple signalling pathways, which lead to varied functional outcomes following CaMKII phosphorylation, depending on the identity and location of the binding partner. A new phosphorylation site on CaMKII (Thr253) has been identified in vivo. Thr253 phosphorylation controls CaMKII purely by targeting, does not effect enzyme activity, and occurs in response to physiological and pathological stimuli in vivo, but only in CaMKII molecules present in specific cellular locations. This new phosphorylation site offers a potentially novel regulatory mechanism for controlling functional responses elicited by CaMKII that are restricted to specific subcellular locations and/or certain cell types, by controlling interactions with proteins that are expressed in the cell at that location.

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“…35,36 The bioactive scaffolds have been well documented to coordinate with some cytokines or growth factors in determining differentiation fates. 37,38 In the present study, our hypothesis was that the MAPK cascade can serve as a mediator by transducing the signals from the scaffolds into the cells, thereby regulating DPSC differentiation. Our findings confirmed this hypothesis, as we showed that ERK1/2 and p38 phosphorylations were higher in DPSCs cultured on DDM and CBB than in those cultured on SIS, whereas ERK1/2 and p38 phosphorylations were higher in DPSCs cultured on SIS than in those cultured on PLGA and Co-CS-HA.…”

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confidence: 99%

Natural Mineralized Scaffolds Promote the Dentinogenic Potential of Dental Pulp Stem Cells Via the Mitogen-Activated Protein Kinase Signaling Pathway

Zhang

Liu

Zhou

et al. 2012

Tissue Engineering Part A

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The selection of a suitable scaffold material is important for dentin tissue regeneration, as the characteristics of biomaterials can potentially influence cell proliferation and differentiation. We compared the effects of different scaffolds on dentin regeneration based on dental pulp stem cells (DPSCs) and investigated the regulatory mechanisms of odontogenic differentiation of DPSCs by these scaffolds. Five different scaffolds were tested: demineralized dentin matrix (DDM), ceramic bovine bone (CBB), small intestinal submucosa (SIS), poly-L-lactate-co-glycolate, and collagen-chondroitin sulfate-hyaluronic acid. DPSCs cultured on DDM and CBB exhibited higher levels of alkaline phosphatase (ALP) activity and mRNA expression of bone sialoprotein, osteocalcin, dentin sialophosphoprotein (DSPP), and dentin matrix protein-1 (DMP-1) than those cultured on the other three scaffolds. Further, the phosphorylation levels of mitogen-activated protein kinase (MAPK) ERK1/2 and p38 in DPSCs cultured on DDM and CBB were also significantly enhanced compared with the other three scaffolds, and their inhibitors significantly inhibited odontogenic differentiation as assessed by ALP activity and mRNA expression of DSPP and DMP-1. The implantation experiment confirmed these results and showed a large amount of regular-shaped dentin-pulp complex tissues, including dentin, predentin, and odontoblasts only in the DDM and CBB groups. The results indicated that natural mineralized scaffolds (DDM and CBB) have potential as attractive scaffolds for dentin tissue-engineering-promoted odontogenic differentiation of DPSCs through the MAPK signaling pathway.

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A novel collagen-binding peptide promotes osteogenic differentiation via Ca2+/calmodulin-dependent protein kinase II/ERK/AP-1 signaling pathway in human bone marrow-derived mesenchymal stem cells

Cited by 64 publications

References 39 publications

Mechanisms for osteogenic differentiation of human mesenchymal stem cells induced by fluid shear stress

Mechanisms for osteogenic differentiation of human mesenchymal stem cells induced by fluid shear stress

Regulation of CaMKII In vivo: The Importance of Targeting and the Intracellular Microenvironment

Natural Mineralized Scaffolds Promote the Dentinogenic Potential of Dental Pulp Stem Cells Via the Mitogen-Activated Protein Kinase Signaling Pathway

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