A novel colony-print immunoassay reveals differential patterns of distribution and horizontal transmission of four unrelated mycoviruses in Rosellinia necatrix

Yaegashi, Hajime; Sawahata, Takuo; Ito, Takumi; Kanematsu, Satoko

doi:10.1016/j.virol.2010.10.014

Cited by 41 publications

(22 citation statements)

References 22 publications

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“…This fact, and the low levels of RnVV1 replication in EP155, suggested that RnVV1 would be eliminated during repeated subculture. This possibility is supported by previous reports indicating that even in natural hosts, some mycoviruses are distributed unevenly and are easily lost during subculture (15,71). However, RnVV1 was readily transmitted via anastomosis (Fig.…”

Section: Discussionsupporting

confidence: 85%

A Novel Victorivirus from a Phytopathogenic Fungus, Rosellinia necatrix, Is Infectious as Particles and Targeted by RNA Silencing

Chiba

Lin

Kondō

et al. 2013

J Virol

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Japan bA novel victorivirus, termed Rosellinia necatrix victorivirus 1 (RnVV1), was isolated from a plant pathogenic ascomycete, white root rot fungus Rosellinia necatrix, coinfected with a partitivirus. The virus was molecularly and biologically characterized using the natural and experimental hosts (chestnut blight fungus, Cryphonectria parasitica). RnVV1 was shown to have typical molecular victorivirus attributes, including a monopartite double-stranded RNA genome with two open reading frames (ORFs) encoding capsid protein (CP) and RNA-dependent RNA polymerase (RdRp), a UAAUG pentamer presumed to facilitate the coupled termination/reinitiation for translation of the two ORFs, a spherical particle structure ϳ40 nm in diameter, and moderate levels of CP and RdRp sequence identity (34 to 58%) to those of members of the genus Victorivirus within the family Totiviridae. A reproducible transfection system with purified RnVV1 virions was developed for the two distinct fungal hosts. Transfection assay with purified RnVV1 virions combined with virus elimination by hyphal tipping showed that the effects of RnVV1 on the phenotype of the natural host were negligible. Interestingly, comparison of the RNA silencing-competent (standard strain EP155) and -defective (⌬dcl-2) strains of C. parasitica infected with RnVV1 showed that RNA silencing acted against the virus to repress its replication, which was restored by coinfection with hypovirus or transgenic expression of an RNA silencing suppressor, hypovirus p29. Phenotypic changes were observed in the ⌬dcl-2 strain but not in EP155. This is the first reported study on the host range expansion of a Totiviridae member that is targeted by RNA silencing.

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Section: Discussionsupporting

confidence: 85%

A Novel Victorivirus from a Phytopathogenic Fungus, Rosellinia necatrix, Is Infectious as Particles and Targeted by RNA Silencing

Chiba

Lin

Kondō

et al. 2013

J Virol

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“…Only intracellular transmissions by hyphal anastomosis and heterokaryosis (horizontal transmission) and spread via sexually or asexually derived spores (vertical transmission) have been observed [5], [33]. Virus dissemination in mycelial networks via dolipores and septa is believed to be a passive phenomenon, as organelles easily migrate in between adjacent cells.…”

Section: Introductionmentioning

confidence: 99%

Codivergence of Mycoviruses with Their Hosts

et al. 2011

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BackgroundThe associations between pathogens and their hosts are complex and can result from any combination of evolutionary events such as codivergence, switching, and duplication of the pathogen. Mycoviruses are RNA viruses which infect fungi and for which natural vectors are so far unknown. Thus, lateral transfer might be improbable and codivergence their dominant mode of evolution. Accordingly, mycoviruses are a suitable target for statistical tests of virus-host codivergence, but inference of mycovirus phylogenies might be difficult because of low sequence similarity even within families.MethodologyWe analyzed here the evolutionary dynamics of all mycovirus families by comparing virus and host phylogenies. Additionally, we assessed the sensitivity of the co-phylogenetic tests to the settings for inferring virus trees from their genome sequences and approximate, taxonomy-based host trees.ConclusionsWhile sequence alignment filtering modes affected branch support, the overall results of the co-phylogenetic tests were significantly influenced only by the number of viruses sampled per family. The trees of the two largest families, Partitiviridae and Totiviridae, were significantly more similar to those of their hosts than expected by chance, and most individual host-virus links had a significant positive impact on the global fit, indicating that codivergence is the dominant mode of virus diversification. However, in this regard mycoviruses did not differ from closely related viruses sampled from non-fungus hosts. The remaining virus families were either dominated by other evolutionary modes or lacked an apparent overall pattern. As this negative result might be caused by insufficient taxon sampling, the most parsimonious hypothesis still is that host-parasite evolution is basically the same in all mycovirus families. This is the first study of mycovirus-host codivergence, and the results shed light not only on how mycovirus biology affects their co-phylogenetic relationships, but also on their presumable host range itself.

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“…As shown in mycoviruses of Rosellinia necatrix, Rosellinia necatrix mycoreovirus 3 and Rosellinia necatrix quadrivirus 1 showed a low transmission efficiency compared with Rosellinia necatrix partitivirus 1 and Rosellinia necatrix megabirnavirus 1 (Yaegashi et al, 2011). However, the transmission capacity for mitoviruses in comparing with other mycoviruses is still not clear.…”

Section: Discussionmentioning

confidence: 93%

Characterization of three mycoviruses co-infecting the plant pathogenic fungus Sclerotinia nivalis

Deng

Zhou

et al. 2016

Virus Research

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A novel colony-print immunoassay reveals differential patterns of distribution and horizontal transmission of four unrelated mycoviruses in Rosellinia necatrix

Cited by 41 publications

References 22 publications

A Novel Victorivirus from a Phytopathogenic Fungus, Rosellinia necatrix, Is Infectious as Particles and Targeted by RNA Silencing

A Novel Victorivirus from a Phytopathogenic Fungus, Rosellinia necatrix, Is Infectious as Particles and Targeted by RNA Silencing

Codivergence of Mycoviruses with Their Hosts

Characterization of three mycoviruses co-infecting the plant pathogenic fungus Sclerotinia nivalis

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