A novel colorimetric biosensor for detecting target DNA and human alpha thrombin based on associative toehold activation concatemer induced catalyzed hairpin assembly amplification

Bai, Shulian; Wang, Ting; Zhang, Zhang; Sheng, Shangchun; Yu, Wen; Xie, Guoming

doi:10.1016/j.snb.2016.08.026

Cited by 31 publications

(8 citation statements)

References 31 publications

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“…The detection of binding kinetics of DNA hybridization plays an important role in the diagnosis of diseases, [1][2][3][4][5] treatment of pathogen infections 3 and forensic tests. 4,5 To date, a variety of methods have been studied for the detection of DNA hybridization which mainly include optical [6][7][8] and electrochemical methods. [9][10][11] Among the optical methods, label-free surface plasmon resonance (SPR) is the standard tool for DNA binding detection.…”

Section: Introductionmentioning

confidence: 99%

An electricity-fluorescence double-checking biosensor based on graphene for detection of binding kinetics of DNA hybridization

et al. 2017

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Section: Introductionmentioning

confidence: 99%

An electricity-fluorescence double-checking biosensor based on graphene for detection of binding kinetics of DNA hybridization

et al. 2017

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“…The detection limit for thrombin concentration (S/N=3) was estimated to be 0.35 fM. Compared to previous developed biosensors for thrombin detection such as electrochemical assay (70 pM), electrochemiluminescence assay (0.2 pM), chemiluminescence assay (6.2 pM), and colorimetric assay (0.68 pM), the designed PEC aptasensor still exhibited a lower detection limit as well as wider linear range, demonstrating its superiority. Additionally, in order to demonstrate synergistic effect of exonuclease aided target recycling, the sensitivity of the aptasensor just without RecJ exonuclease incubation was measured.…”

Section: Resultsmentioning

confidence: 99%

Signal‐On Photoelectrochemical Aptasensor Amplified by Exciton Energy Transfer and Exonuclease‐Aided Target Recycling

et al. 2017

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An enhanced photoelectrochemical (PEC) aptasensor was developed by coupling exciton energy transfer (EET) with exonuclease aided target recycling. Thrombin was selected as the target analyte. CdS quantum dots (QDs) as energy donors were first modified on the TiO2 film to form a TiO2/CdS heterostructure, and then complementary DNA (cDNA) of the thrombin aptamer probe (pDNA) was immobilized. Au nanoparticles (AuNPs) as energy acceptors were labeled at the 3′ end of pDNA to form AuNP‐pDNA composites. After pDNA hybridized with cDNA, the EET occurred and the photocurrent reduced evidently. When the mixture of thrombin and RecJ exonuclease was introduced, pDNA specifically bound with thrombin, leading to the separation of AuNP‐pDNA composites from the electrode. As RecJ exonuclease could cleave single‐strand pDNA from 5′–3′, the captured thrombin was set free and it recurrently binds with the rest of pDNA. As a result, the EET was broken, to a large extent, and the photocurrent clearly picked up. This signal‐on PEC aptasensor shows a low detection limit and good selectivity for target analysis.

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“…The released target can hybridize with another hairpin 1 and will be replaced by another hairpin 2 and so on. The hairpin 2 has a signaling tag that allows for recognition with a substrate that produces a signal, proportional to the target concentration [ 100 , 101 ]. Shuai et al developed a biosensor for detecting microRNA based on (CHA) (see Figure 2 ).…”

Section: Nanobioconjugates In Biosensingmentioning

confidence: 99%

Nanobioconjugates for Signal Amplification in Electrochemical Biosensing

Cajigas

Orozco

2020

Molecules

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Nanobioconjugates are hybrid materials that result from the coalescence of biomolecules and nanomaterials. They have emerged as a strategy to amplify the signal response in the biosensor field with the potential to enhance the sensitivity and detection limits of analytical assays. This critical review collects a myriad of strategies for the development of nanobioconjugates based on the conjugation of proteins, antibodies, carbohydrates, and DNA/RNA with noble metals, quantum dots, carbon- and magnetic-based nanomaterials, polymers, and complexes. It first discusses nanobioconjugates assembly and characterization to focus on the strategies to amplify a biorecognition event in biosensing, including molecular-, enzymatic-, and electroactive complex-based approaches. It provides some examples, current challenges, and future perspectives of nanobioconjugates for the amplification of signals in electrochemical biosensing.

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A novel colorimetric biosensor for detecting target DNA and human alpha thrombin based on associative toehold activation concatemer induced catalyzed hairpin assembly amplification

Cited by 31 publications

References 31 publications

An electricity-fluorescence double-checking biosensor based on graphene for detection of binding kinetics of DNA hybridization

An electricity-fluorescence double-checking biosensor based on graphene for detection of binding kinetics of DNA hybridization

Signal‐On Photoelectrochemical Aptasensor Amplified by Exciton Energy Transfer and Exonuclease‐Aided Target Recycling

Nanobioconjugates for Signal Amplification in Electrochemical Biosensing

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