“…To this end, researchers have used antibodies or antigens in the same assay zone labeled with (a) different fluorescent molecules (6,7), (b) different radioactive species (8 -10), (c) different enzymes (11,12), (d) a mixture of radioactive species and enzymes (13), (e) metal ions (14), (f) colored latex particles (15), and (g) particles of different sizes (16). The various detection schemes used in these multianalyte assays are based on a change in one or more of the following signals: absorbance, steady-state fluorescence, fluorescence lifetime, chemiluminescence, radioactivity, electrochemical response, laser light scattering, and frequency of apiezoelectric quartz crystal, upon the binding event(s) (6 -20).…”