Multianalyte Detection Using a Capillary-Based Flow Immunosensor

Narang, Upvan; Gauger, Paul R.; Kusterbeck, Anne W.; Ligler, Frances S.

doi:10.1006/abio.1997.2411

Cited by 78 publications

(57 citation statements)

References 28 publications

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“…Many reviews detailing the research and development in the field of optical-based immunoassays have been published (14,15). A number of FIA have been developed for the detection of explosive materials (16), polychlorinated biphenyls (PCBs) (17), and atrazine (18). The configurations employed in these studies included antibody saturated with corresponding fluorescent-labeled antigen immobilized onto either a decladed optical fiber, which is integrated to a fluorometer, or the inside walls of a capillary flow column placed upstream of a fluorescence detector.…”

mentioning

confidence: 99%

Development of a Fluorescence Immunoassay for Measurement of Paclitaxel in Human Plasma

Sheikh

Abela

Mulchandani

2000

Analytical Biochemistry

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mentioning

confidence: 99%

Development of a Fluorescence Immunoassay for Measurement of Paclitaxel in Human Plasma

Sheikh

Abela

Mulchandani

2000

Analytical Biochemistry

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“…36 Fiber optic evanescent wave immunosensors have been developed for the detection of toxins, hazardous materials, explosives, and clinical analytes using monoclonal and poly clonal antibodies. [37][38][39][40] Human chorionic gonadotropin was detected through planar waveguide fluorescent immunosensors by sandwich assay using monoclonal antibody. 41,42 Comparatively, the newly developed assay significantly detect the ER␣ protein by direct immuno binding.…”

Section: Discussionmentioning

confidence: 99%

Highly sensitive optical detection of specific protein in breast cancer cells using microstructured fiber in extremely low sample volume

Padmanabhan

Shinoj

Murukeshan³

et al. 2010

J. Biomed. Opt.

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Abstract.A simple optical method using hollow-core photonic crystal fiber for protein detection has been described. In this study, estrogen receptor ͑ER͒ from a MCF-7 breast carcinoma cell lysates immobilized inside a hollow-core photonic crystal fiber was detected using anti-ER primary antibody with either Alexa ™ Fluor 488 ͑green fluorescent dye͒ or 555 ͑red Fluorescent dye͒ labeled Goat anti-rabbit IgG as the secondary antibody. The fluorescence fingerprints of the ER␣ protein were observed under fluorescence microscope, and its optical characteristics were analyzed. The ER␣ protein detection by this proposed method is based on immuno binding from sample volume as low as 50 nL. This method is expected to offer great potential as a biosensor for medical diagnostics and therapeutics applications.

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“…Other classes of protein arrays under development include gelimmobilized arrays (Vasiliskov et al, 1999) and fiber optic sensing arrays (Shriver-Lake et al, 1993;Wadkins et al, 1998), multiplexed capillary-based flow immunosensors and multiplexed microbead antibody capture technologies (Fulton et al, 1997;Narang et al, 1998;Carson et al, 1999). These technologies add a third-dimensional space (spherical, cylindrical or elongated domains), thereby expanding the surface area available for interaction.…”

Section: B) Protein Pathway Profilingmentioning

confidence: 99%

Role of Genetic and Expression Profiling in Pharmacogenomics: The Changing Face of Patient Management

Pagliarulo

Datar

Côté

2002

Current Issues in Molecular Biology

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As the determination of gene sequences and their function gains speed at the dawn of the third millennium, biomedical research efforts are oriented towards definition of the genetic and molecular expression patterns that may drive different disease. A major part of these efforts is addressed to the definition of inter-individual variations that are expected to become integral for treatment planning, in terms of efficacy and adverse effects of drugs. It is this thrust on genome-based 'rational therapeutics' that is hoped to progressively lead to the era of 'personalized medicine'. This approach uses the technological expertise from genomics and functional genomics to define, predict and monitor the nature of the response of an individual to drugs, and to rationally design newer drugs. In the present review we will conduct our readers through an understanding of the fundamentals of pharmacogenomics and of the technologies currently available that are advancing this relatively new science. Conversely, there are issues raised that concern how medical practice is preparing itself to implement new alternatives for therapeutical interventions and finally, how to respect patient confidentiality and rights.

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Multianalyte Detection Using a Capillary-Based Flow Immunosensor

Cited by 78 publications

References 28 publications

Development of a Fluorescence Immunoassay for Measurement of Paclitaxel in Human Plasma

Development of a Fluorescence Immunoassay for Measurement of Paclitaxel in Human Plasma

Highly sensitive optical detection of specific protein in breast cancer cells using microstructured fiber in extremely low sample volume

Role of Genetic and Expression Profiling in Pharmacogenomics: The Changing Face of Patient Management

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