A novel conducting copolymer: Investigation of its matrix properties for cholesterol biosensor applications

A novel paper-based device coupled with a silver nanoparticle-modified boron-doped diamond electrode for cholesterol detection

“…Previously, several methods have been used to immobilize the enzyme onto an electrode, such as entrapment in a polymer [9], covalent linkage with glutaraldehyde [10] or a polymer [11], and electrostatic adsorption [12]. Although these sensing platforms provide good performance for cholesterol detection, limitations still remain in terms of time consumption, detection performance and the ease with which the enzyme is denatured during its immobilization.…”

Fabrication of a nonenzymatic cholesterol biosensor using carbon nanotubes from coconut oil

“…The cholesterol biosensors based on carbon nanotube (CNT) have been continuously reported [8][9][10][11][12][13][14][15][16][17]. It has been demonstrated that carbon nanotubes could promote electron transfer with various redox active proteins, ranging from cytochrome c to glucose oxidase with a deeply embedded redox center and horseradish peroxidase with surface redox centers.…”

Fabrication of Chiral Poly(3,4-ethylenedioxythiophene) Derivatives Modified Glassy Carbon Electrodes for Ascorbic Acid Determination

“…In bioactive platform preparation, EDC and NHS were used as cross linkers to obtain a covalent binding between amino groups of the polymer and carboxylic acid groups of the enzyme molecules [15][16][17]. The enzyme solution that contained 3 µL (3.33 U) AO, 3 µL 0.4 M EDC and 3 µL 0.1 M NHS was dip-coated on the surface of the polymer coated electrode [18].…”