A novel cytotoxic protein, Karatoxin, from the dorsal spines of the redfin velvetfish,<i>Hypodytes rubripinnis</i>

Nagasaka, Kuniko; Nakagawa, Hideyuki; Satoh, Fumihiko; Hosotani, Takefumi; Yokoigawa, Kumio; Sakai, Hitomi; Sakuraba, Haruhiko; Ohshima, Toshihisa; Shinohara, Mitsuko; Ohura, Kiyoshi

doi:10.3109/15569540903348940

Cited by 11 publications

(12 citation statements)

References 13 publications

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“…The partial amino acid sequence was determined up to 5 residues, and found to be DHEDS. In the case of the waspfish Hypodytes rubripinnis, Karatoxin, a novel cytotoxic protein (110 kDa), was successfully purified from the dorsal spines of this fish (Nagasaka et al 2009;Shinohara et al 2010). The N-terminal partial amino acid sequence of the 76 kDa subunit of Karatoxin was shown to be DQHDDxPxxAPDPG.…”

Section: Resultsmentioning

confidence: 98%

“…The collected fish were transported alive or frozen to our laboratory. The dorsal spines (a total of 17) of I. japonicus were cut from their base, and the dorsal venom protein was extracted with 0.15 M NaCl as reported previously (Nagasaka et al 2009). Briefly, in the first step of purification, the venom protein was applied to a Phenyl Sepharose CL-4B (GE Healthcare, Uppsala, Sweden) affinity chromatographic column (2 ml) equilibrated with 16 mM TrisHCl buffer containing 2 M NaCl (pH 7.4).…”

Section: Isolation Of a Dorsal Lectinmentioning

confidence: 99%

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Isolation of a novel lectin from the dorsal spines of the devil stinger, Inimicus japonicus

et al. 2015

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A novel lectin was purified from the dorsal spines of the devil stinger, Inimicus japonicus using a combination of affinity chromatography techniques. A single band was detected on a native PAGE gel with a relative molecular mass of 97 kDa. The N-terminal partial amino acid of the intact 75 kDa subunit of the 97 kDa lectin was found to be DHEDS. The agglutination of rabbit erythrocytes by the 97 kDa lectin was inhibited most effectively by methyl a-D-mannoside. The 97 kDa lectin stimulated mitogenesis in murine splenocytes. This is the first study to examine the dorsal lectin of I. japonicus and one of the very few studies on venom lectins from venomous scorpaeniform fish. These results suggest that the devil stinger, I. japonicus, may be a novel resource for biologically active substances.

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Section: Resultsmentioning

confidence: 98%

Section: Isolation Of a Dorsal Lectinmentioning

confidence: 99%

Isolation of a novel lectin from the dorsal spines of the devil stinger, Inimicus japonicus

et al. 2015

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“…Karatoxin is mainly composed of 76-and 30-kDa subunits. The N-terminal 14 amino acid sequence of the 76-kDa subunit was shown to be DQHDDxPxxAPDPG (2). The amino acid sequence of Karatoxin is unique and is not related to other fish venoms (12,13).…”

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confidence: 99%

“…However, there is little information available regarding the biochemical and physiological properties of the venom from H. rubripinnis . More recently, we observed that Karatoxin, a glycoprotein purified from the dorsal venom, shows cytotoxic activity against murine P388 leukemia cells (2).…”

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confidence: 99%

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A Novel Chemoattractant Lectin, Karatoxin, From the Dorsal Spines of the Small Scorpionfish Hypodytes rubripinnis

et al. 2010

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Abstract.A chemoattractant lectin from the dorsal spines of the redfin velvetfish, Hypodytes rubripinnis , was isolated using a combination of affinity chromatography techniques. The glycoprotein, with a molecular mass of 110 kDa, is named Karatoxin. Karatoxin caused agglutination of rabbit erythrocytes. This agglutination was effectively inhibited by D -mannose. In addition, Karatoxin exhibited not only mitogenic activity in the presence of murine splenocytes, but also chemotactic activity in the presence of guinea-pig neutrophils and macrophages. Thus, Karatoxin appears to be a novel chemoattractant lectin. These results suggest that the redfin velvetfish Hypodytes rubripinnis may be a novel source of biologically active substances.

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The piscine arsenal: an updated review of venomous fishes

Harris

2023

Rev Fish Biol Fisheries

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A novel cytotoxic protein, Karatoxin, from the dorsal spines of the redfin velvetfish,Hypodytes rubripinnis

Cited by 11 publications

References 13 publications

Isolation of a novel lectin from the dorsal spines of the devil stinger, Inimicus japonicus

Isolation of a novel lectin from the dorsal spines of the devil stinger, Inimicus japonicus

A Novel Chemoattractant Lectin, Karatoxin, From the Dorsal Spines of the Small Scorpionfish Hypodytes rubripinnis

The piscine arsenal: an updated review of venomous fishes

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