A novel, efficient and sensitive method for the simultaneous determination of riboflavin (vitamin B2) and pyridoxine hydrochloride (vitamin B6) in food and pharmacological samples using an electrochemical sensor based on 4,4′-diamino benzophenone

Calam, Tuğba Tabanlıgil

doi:10.1016/j.microc.2021.106557

Cited by 26 publications

(8 citation statements)

References 47 publications

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“…The regression equation for the dependence of the peak potential on the pH is given by the equation (Figure 8B): Ep = −0.063 pH +1.18 (R 2 = 0.994). The slope is close to the Nernstian value of −59 mV, indicating the same number of protons and electrons are involved in the electrochemical oxidation of VB6, which is in agreement with previous works [4,5,20]. Scan rate study By using cyclic voltammetry, the influence of scan rate on the oxidation peak current and peak potential of 200 M VB6 in ABS pH 4.5 at the poly(phenylalanine)/F-MWCNT/GCE was investigated in order to determine the electrochemical reaction mechanism of VB6 at the modified electrode (Figure 9A).…”

Section: Impact Of Solution Phsupporting

confidence: 92%

“…Water-soluble vitamins are not stored in the body due to easy discharge through urine [2,4]. Furthermore, they cannot be synthesized in the human body [3,5]. Therefore, they must be obtained from dietary sources for normal cell growth.…”

Section: Introductionmentioning

confidence: 99%

“…Among the water-soluble vitamins, VB6 (pyridoxine) (Figure 1A) plays an important role in body metabolism. VB6 is involved in amino acid metabolism, red cell production, hemoglobin synthesis, gene expression, enzymecatalyzed reactions and nervous and immune systems [1,5]. VB6 is found in many foods such as chickpeas, turkey, fish, starchy vegetables, potatoes, bananas, meats, organ meats, fortified cereals and whole grains [6,7].…”

Section: Introductionmentioning

confidence: 99%

“…For the purpose of determining VB6, a number of analytical techniques, including liquid chromatography [8], gas chromatography-mass spectrometry [9], chemiluminescence [10], capillary zone electrophoresis [11] and spectrophotometry [12] have been described in the literature. Many of these analytical techniques require tedious and time-consuming procedures and involve highly sophisticated instrumentation and toxic organic reagents [5,13,14]. Due to great selectivity, sensitivity, quick response time, ease of use and low cost, electrochemical methods have received a lot of interest in electroanalysis [4,15].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Electrochemical determination of vitamin B6 in pharmaceutical and energy drink samples

Tesfaye¹,

Tessema²,

Negash³

2023

J. Electrochem. Sci. Eng.

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A simple and low-cost electrochemical sensor based on poly(phenylalanine) and functionnalized multi-walled carbon nanotubes (F-MWCNTs) modified glassy carbon electrode (GCE) was developed for the determination of vitamin B6 (VB6). The surface morphology of modified glassy carbon electrodes was investigated with scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). The electrocatalytic activities of the bare and modified electrodes were investigated in the presence of ferri-ferrocyanide redox couple using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The exchange current density (jo = 2462 µA cm-2) and electron transfer rate constant (ko = 0.002 cm s−1) were calculated using 5 mM K3[Fe(CN)6]. The electrochemical activity of poly(phenylalanine)/F-MWCNT/GCE towards VB6 oxidation was investigated using CV. Parameters including the number of electrons transferred (n = 2), number of protons transferred (H+ = 2), electron transfer coefficient (α = 0.51) and surface concentration of VB6 (G = 0.24 nmol cm−2) were calculated. At the optimal experimental conditions, the oxidation peak current of VB6 measured by square wave voltammetry (SWV) was found proportional to its concentration in two linear ranges of 0.5 to 20 µM and 20 to 200 µM with a low detection limit (LOD) of 0.038 µM and limit of quantification (LOQ) of 0.125 µM. Finally, the sensor was successfully used to determine VB6 in soft drink and pharmaceutical formulation samples.

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Section: Impact Of Solution Phsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Electrochemical determination of vitamin B6 in pharmaceutical and energy drink samples

Tesfaye¹,

Tessema²,

Negash³

2023

J. Electrochem. Sci. Eng.

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“… 7 In the organisms, the main physiological function of VB 2 is a coenzyme that can promote energy metabolism and biological oxidation–reduction processes, such as the metabolism of fat, carbohydrate and protein. 8 Adding RF into supplemental intake or adequate dietary will have biological protective effects in terms of anti-aging, 9 anti-inflammatory, 10 antioxidant, 11 and anticancer properties. 12 Generally speaking, excess or lack of RF is harmful to the normal metabolism of the organism.…”

Section: Introductionmentioning

confidence: 99%

An innovative study on the “on–off–on” detection of sulfur ions based on a TSPP–riboflavin fluorescent probe

Wang

Liu

et al. 2022

RSC Adv.

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Electrochemical Behavior and Ultrasensitive, Simple and Effective Voltammetric Determination of Acetaminophen Using Modified Glassy Carbon Electrode Based on 4‐Hydroxyquinoline‐3‐carboxylic Acid

Uzun

Calam

2022

Electroanalysis

Self Cite

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An electrochemical oxidation of acetaminophen (ACOP) has been successfully performed by using glassy carbon electrode covered with 4-hydroxyquinoline-3carboxylic acid (4HQ3CA) to reinforce electrode's feature. To characterize the modified electrode (4HQ3CA/ GC), electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and Fourier transform infrared spectroscopy (FT-IR) techniques were used. The finding optimum conditions (supporting electrolyte, pH) and the electrochemical determination studies were performed with differential pulse voltammetry (DPV). It was decided that the supporting electrolyte medium suitable for ACOP determination was Britton-Robinson (BR) buffer and the effect of pH change on the oxidation peak of ACOP in this media was investigated. The effect of changing scan rate on the oxidation peak of ACOP was examined and this study showed that the oxidation process of ACOP on the 4HQ3CA/GC modified electrode surface was diffusion and adsorption controlled process. A wide concentration range from 0.0025 μM to 141 μM with a limit of detection (LOD) of 5.98 × 10 À 10 M (3 s/m) was obtained. This prepared sensor was carried out for the determination of ACOP in pharmaceutical sample.

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A novel, efficient and sensitive method for the simultaneous determination of riboflavin (vitamin B2) and pyridoxine hydrochloride (vitamin B6) in food and pharmacological samples using an electrochemical sensor based on 4,4′-diamino benzophenone

Cited by 26 publications

References 47 publications

Electrochemical determination of vitamin B6 in pharmaceutical and energy drink samples

Electrochemical determination of vitamin B6 in pharmaceutical and energy drink samples

An innovative study on the “on–off–on” detection of sulfur ions based on a TSPP–riboflavin fluorescent probe

Electrochemical Behavior and Ultrasensitive, Simple and Effective Voltammetric Determination of Acetaminophen Using Modified Glassy Carbon Electrode Based on 4‐Hydroxyquinoline‐3‐carboxylic Acid

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