A novel electrochemical DNA detection method based on bio-barcode/gold label silver stain dual amplification

Abstract

A novel electrochemical gene detection method based on bio-barcode/gold label silver stain (GLSS) dual amplification is presented. The silica nanoparticle/gold nanoparticles (SiO2/AuNPs) barcode probes were constructed by combining SiO2 nanoparticle-labeled DNA with AuNPs, which hybridizes with target DNAs and the electrode surface-modified DNA probes. The silver ions were reduced on the surface of AuNPs to form a silver shell by the catalysis of AuNPs, and then the electrochemical detection of deposited silver was performed by linear sweep voltammetry (LSV). In this paper, the effects of electrode blocking agent, blocking time and silver deposition time on electrochemical detection were also investigated. The proposed electrochemical DNA biosensor bring forth a low detection limit of 0.23 fM and a widely linear range of 1 fM to 10 pM. This method can clearly distinguish the complementary and mismatched DNA, with good repeatability and stability.

Lysozyme-Decorated Gold and Molybdenum Bimetallic Nanoclusters for the Selective Detection of Bilirubin as a Jaundice Biomarker

“…Nanomaterials have been explored as outstanding probes for the identification of a wide variety of analytes from various matrixes. ,− Various metal NCs, including Au NCs, , silver (Ag) NCs, Cu NCs, and platinum (Pt) NCs, , have been applied as promising fluorophores for the sensing of metal ions, drugs, and biomolecules with high selectivity. Apart from these, bimetallic NCs have been utilized in various analytical method developments for the facile and rapid assay of chemical species because of their spatial arrangement as well as atomic ordering of two metal atoms .…”