Lysozyme-Decorated Gold and Molybdenum Bimetallic Nanoclusters for the Selective Detection of Bilirubin as a Jaundice Biomarker

Abstract

Fluorescent nanomaterials have been widely used as sensing probes for the recognition of biological active species or biomarkers. Herein, we have synthesized fluorescent gold and molybdenum bimetallic nanoclusters (Au−Mo NCs) via a one-step reaction using lysozyme (Lys) as a template. The as-synthesized Lys−Au−Mo NCs acted as a fluorescent probe for the specific detection of bilirubin as a jaundice biomarker. The as-fabricated Lys−Au−Mo NCs exhibit a strong emission peak at 642 nm when excited at 490 nm. The Lys−Au−Mo NCs-based fluorescence method exhibited good sensing ability for the detection of bilirubin in the range of 0.125−40.00 μM with a detection limit of 28.26 nM. Furthermore, the as-fabricated Lys−Au−Mo NCs offer a facile and cost-effective analytical strategy for the sensitive identification of bilirubin in biofluids, offering a promising optical probe for the quantification of bilirubin in clinical samples as a jaundice biomarker.

Perspectives of different colour‐emissive nanomaterials in fluorescent ink, LEDs, cell imaging, and sensing of various analytes

“…Schematic representation for the synthesis of Lys-Au-Mo NCs and application for the detection of bilirubin. Reprinted with permission from reference [174] overlapped with the excitation spectra of Lys-Au-Mo NCs and method and they detected that chlorpyrifos and quinalphos pesticides formed fluorescence 'turn-off' and 'turn-on' mechanisms. [175] TEF CDs contained -OH, -NH, and -CN groups which bind to pesticides, confirmed by FT-IR spectra.…”

Supramolecular Self-Assembly of Atomically Precise Silver Nanoclusters with Chiral Peptide for Temperature Sensing and Detection of Arginine

“…As a bridge between atom and nanoparticle, metal nanoclusters (metal NCs, mainly Au, Ag and Cu) which are composed of a few to hundreds of mental atoms and covered with organic ligands on the surface of the metal core, have been receiving extensive attention due to their important roles in the fields of catalysis, sensing, electrochemistry, energy transfer and biomedicine [ 1 , 2 , 3 , 4 , 5 ]. Among various applications, metal nanoclusters are widely used as sensors because of their sensitive responses to temperature, pH value, metal ions and small biological molecules [ 6 , 7 , 8 , 9 , 10 ]. For example, Hu et al utilized Cu-MOF as a precursor to prepare highly stable Cu NCs, which can be used to construct a pH ratiometric fluorescence sensor to monitor pH of microorganism [ 8 ].…”

Sustainable chemistry approach for the preparation of bluish green emissive copper nanoclusters fromJusticia adhatodaleaves extract: a facile analytical approach for the sensing of myoglobin andl-thyroxine

“…20 Lysozyme-mediated Au-Mo NCs were used as analytical sensors for the sensitive detection of biomarker bilirubin. 21 Pepsin is used as a ligand for the synthesis of Au 22 and Cu 23 NCs for the sensing of trace level chemicals and imaging of cells. These studies showed that biopolymers acted as promising molecules for the synthesis of different color emissive metal NCs.…”