A novel electrochemiluminescence choline biosensor based on biofunctional AMs-ChO biocomposite

Wu, Xiaoping; Yuan, Ruo; Liang, Wanjun; Yuan, Dengyue

doi:10.1016/j.snb.2014.07.125

Cited by 16 publications

(6 citation statements)

References 41 publications

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“…For example, Wu's group constructed a choline biosensor based on Fe 3 O 4 and TiO 2 using a luminol/H 2 O 2 system. 9 Song's group constructed an ECL biosensor based on a Chox/titanate nanotube/chitosan composite for choline detection with luminol as a luminophor. 10 However, the application of the above solution-state luminol ECL systems for choline sensing was limited due to the large consumption of ECL reagents, high cost and environmental pollution.…”

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confidence: 99%

Anodic electrogenerated chemiluminescence behavior and the choline biosensing application of blue emitting conjugated polymer dots

Chen

Liao

et al. 2016

Chem. Commun.

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confidence: 99%

Anodic electrogenerated chemiluminescence behavior and the choline biosensing application of blue emitting conjugated polymer dots

Chen

Liao

et al. 2016

Chem. Commun.

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“…CHOD on the surface of the poly(aniline‐luminol‐hemin)/CHOD/graphite electrode can produce H 2 O 2 and betaine in the presence of choline and oxygen, and the H 2 O 2 can react with the polymeric luminol in the poly(aniline‐luminol‐hemin) nanocomposites to produce enhanced ECL signals. Moreover, the ECL reaction of H 2 O 2 and luminol on the electrode surface can be catalyzed by the Fe(III) in hemin to further amplify the ECL signals . Therefore, the ECL intensity increases with the increment of choline concentration.…”

Section: Resultsmentioning

confidence: 99%

“…Since choline that synthesized by the organism is limited, the additional quantities of choline need to be obtained from vitamin formula, infant food and sport drinks. Choline has become a commonly food additive which was widely used in our daily life ,. Choline is now considered as a safe food additive.…”

Section: Introductionmentioning

confidence: 99%

“…Luminol is one of the most commonly used ECL reagents , which has been widely employed to develop sensitive biosensors allowing detection of hydrogen peroxide (H 2 O 2 ). Especially in the field of enzyme biosensors in bioanalytical application, the ECL signals of luminol are amplified by H 2 O 2 generated during the oxidation process of the substrate with enzymatic reaction . Therefore, the ECL biosensors based on luminol can greatly enlarge the application of enzyme biosensors and have been used in the detection of choline .…”

Section: Introductionmentioning

confidence: 99%

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A Highly Sensitive Electrochemiluminescence Choline Biosensor Based on Poly(aniline‐luminol‐hemin) Nanocomposites

et al. 2019

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Poly(aniline‐luminol‐hemin) nanocomposites are prepared on an electrode surface through electropolymerization, and a highly sensitive electrochemiluminescence (ECL) biosensor for choline is developed based on the poly(aniline‐luminol‐hemin) nanocomposites and an enzyme catalyzed reaction of choline oxidase (CHOD). The obtained nanocomposites are characterized by scanning electron microscopy (SEM), atomic absorption spectrometry (AAS) and ECL. The results indicate that hemin can be incorporated into the poly(aniline‐luminol) nanocomposites using the facile electropolymerization method, and the poly(aniline‐luminol‐hemin) nanocomposites are rod shaped porous nanostructure. Moreover, the poly(aniline‐luminol‐hemin) nanocomposites exhibit higher ECL intensity than poly(aniline‐luminol) nanocomposites in alkaline media due to the catalytic effect of hemin on the ECL of the polymerized luminol and the electron transfer ability of hemin in the nanocomposites. CHOD is immobilized on the surface of the poly(aniline‐luminol‐hemin) nanocomposites modified electrode with glutaraldehyde, and the ECL biosensor based on poly(aniline‐luminol‐hemin)/CHOD exhibits a wider linear range for the choline detection. The enhanced ECL signals are linear with the logarithm of concentration of choline over the range of 1.0×10−11∼1.0×10−7 mol L−1 with a low detection limit of 1.2×10−12 mol L−1. Moreover, the proposed biosensor is successfully applied to the detection of choline in milk.

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“…Recently, various Ch measurement strategies have been reported, including fluorescence and colorimetric methods [11,12], chemiluminescence [13,14], liquid chromatography [15], and capillary electrophoresis [16]. Although these methods have significant sensitivity and selectivity, the requirement of complicated procedures and devices, professional operations, and pre-treatments of samples restrict their application in real-time detection [17].…”

Section: Introductionmentioning

confidence: 99%

Protoporphyrin IX Based All-Solid-State Ion-Selective Electrodes for Choline Determination In Vitro

Wang

Zou

et al. 2021

Applied Sciences

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Protoporphyrin IX-based all-solid-state choline (Ch) ion-selective electrodes (ISEs) were fabricated and characterized. Poly (3,4-ethylene dioxythiophene) doped with poly (styrene sulfonate) (PEDOT/PSS) functioning as an ion-to-electron transducer was electropolymerized on the gold wire (0.5 mm diameter). The conductive polymer was covered with a Ch selective membrane containing protoporphyrin IX as an ionophore, which exhibited a lower detection limit of 0.49 μM with the potentiometric method. The Ch sensor performed a wide linear range from 1 μM to 1 mM, a fast response time of less than 5 s, and a decent selectivity of common inorganic and organic ions in the human body. Characteristics such as pH and temperature stability, life span, reproducibility and repeatability were also investigated to be satisfied. With the background of artificial cerebrospinal fluid, the recovery rate in 10−5 M of Ch solution was measured by the standard addition method, revealing the potential for biological application.

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A novel electrochemiluminescence choline biosensor based on biofunctional AMs-ChO biocomposite

Cited by 16 publications

References 41 publications

Anodic electrogenerated chemiluminescence behavior and the choline biosensing application of blue emitting conjugated polymer dots

Anodic electrogenerated chemiluminescence behavior and the choline biosensing application of blue emitting conjugated polymer dots

A Highly Sensitive Electrochemiluminescence Choline Biosensor Based on Poly(aniline‐luminol‐hemin) Nanocomposites

Protoporphyrin IX Based All-Solid-State Ion-Selective Electrodes for Choline Determination In Vitro

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