A novel electrochemiluminescent immunosensor based on the quenching effect of aminated graphene on nitrogen-doped carbon quantum dots

Zhou, Jing; Han, Tongqian; Ma, Hongmin; Yan, Tao; Pang, Xuehui; Li, Yueyun; Wei, Qin

doi:10.1016/j.aca.2015.07.018

Cited by 65 publications

(24 citation statements)

References 37 publications

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“…As a wide strategy used in luminescence techniques, RET opens up a new avenue to fabricate ECL sensors with more flexibility, such as distance-dependent ECL assay and spectrabased ratiometric ECL. 125 Due to the tunable luminescence properties of GDs, Tian et al developed an ECL-RET system with N-doped GDs as the acceptor and luminol as the donor. 126 Notably, the luminol and N-doped GD hybrids can generate anodic ECL signals without coreactants.…”

Section: +mentioning

confidence: 99%

Carbon-based dots for electrochemiluminescence sensing

Chen

Cao

et al. 2020

Mater. Chem. Front.

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Section: +mentioning

confidence: 99%

Carbon-based dots for electrochemiluminescence sensing

Chen

Cao

et al. 2020

Mater. Chem. Front.

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“…Electrochemiluminescent C-dots have applied to AFP detection 24,25 . In our previous work, fluorescent C-dots were directly applied as biolabels on antibodies in an immunosorbent assay to detect AFP 26 .…”

Section: Introductionmentioning

confidence: 99%

A novel ratiometric fluorescent immunoassay for human α-fetoprotein based on carbon nanodot-doped silica nanoparticles and FITC

Peng

Pengpumkiat

et al. 2016

Anal. Methods

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The application of fluorescent carbon nanodots (C-dots or CD), non-toxic particulate organic labels, to disease biomarker detection is still in its earliest stage of development. In the effort described here, a novel ratiometric immunoassay was developed to target a model protein disease biomarker, alpha-fetoprotein (AFP), using C-dot doped silica nanoparticles (CD-SNPs) and fluorescein isothiocyanate (FITC) as signaling agents. Highly fluorescent C-dots were hydrothermally synthesized from citric acid and ethylene diamine. The C-dots were then encapsulated in silicate shells to yield 45 nm nanoparticles using a novel reverse microemulsion method, enabling convenient handling (centrifugation and washing) and straightforward surface chemistry modification to facilitate development of bioassays. Capture antibody capped CD-SNPs (Ab1-CD-SNPs), together with FITC labeled antibodies (Ab2-FITC) constituted a new ratiometric immunosensor for AFP, in which CD-SNPs functioned as both solid supports for washing and separation and as built-in reference signaling agents to correct for inconsistent environmental effects. A calibration curve was established between the ratiometric signal (the ratio of fluorescence signals of FITC and C-dots, F/C) and AFP concentration in the broad range of 0.317-280 μg/dL, exhibiting a useful linear range (0.317-35 μg/dL, R 2 =0.9977), low detection limit (0.317 μg/dL) and acceptable recovery (105-120%). This assay format can be applied to a wide range of immunoassay targets. Our demonstration of encapsulating low-cost, easily synthesized, highly-fluorescent C-dots into silica nanoparticles for use in immunoassays will be useful in expanding future applications of these carbon nanomaterials to areas such as in-vivo cellular imaging, drug delivery, and in-vitro cell labeling and biomolecule sensing.

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“…Additionally, carbon and graphene quantum dots can be applied for fabricating of immuno-sensor. For instance, an immuno-sensor was prepared for 8-hydroxy-2′-deoxyguanosine (8-OHdG) where carbon quantum dots coated with gold/SiO 2 coreshell nanoparticles were immobilized onto a platinum electrode (Zhou et al 2015).…”

Section: Biosensorsmentioning

confidence: 99%

Green synthesis, biomedical and biotechnological applications of carbon and graphene quantum dots. A review

2020

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Carbon and graphene quantum dots are prepared using top-down and bottom-up methods. Sustainable synthesis of quantum dots has several advantages such as the use of low-cost and non-toxic raw materials, simple operations, expeditious reactions, renewable resources and straightforward post-processing steps. These nanomaterials are promising for clinical and biomedical sciences, especially in bioimaging, diagnosis, bioanalytical assays and biosensors. Here we review green methods for the fabrication of quantum dots, and biomedical and biotechnological applications.

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A novel electrochemiluminescent immunosensor based on the quenching effect of aminated graphene on nitrogen-doped carbon quantum dots

Cited by 65 publications

References 37 publications

Carbon-based dots for electrochemiluminescence sensing

Carbon-based dots for electrochemiluminescence sensing

A novel ratiometric fluorescent immunoassay for human α-fetoprotein based on carbon nanodot-doped silica nanoparticles and FITC

Green synthesis, biomedical and biotechnological applications of carbon and graphene quantum dots. A review

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