A novel carboxamide-based colorimetric chemosensor ATA-Msal ((E)-3-((2-hydroxy-3-methoxybenzylidene)amino) thiophene-2-carboxamide) for detecting Cu 2 + and Co 2 + in aqueous solution was synthesized. Sensor ATA-Msal could probe Cu 2 + and Co 2 + by color change from colorless to pale yellow. According to results of Job plot and ESI-mass, the binding modes of ATA-Msal to Cu 2 + and Co 2 + were determined to be a 1 : 1 ratio. The detection limits (0.14 μM and 0.88 μM) of ATA-Msal for Cu 2 + and Co 2 + were much lower than WHO standard (31.5 μM for Cu 2 + ) and US Environmental Protection Agency (EPA) standard (1.70 μM for Co 2 + ). ATA-Msal can be applicable to real samples and test strips, and F À could be used to distinguish between ATA-Msal -Cu 2 + and ATA-Msal -Co 2 + . The sensing process of Cu 2 + and Co 2 + by ATA-Msal was demonstrated by UV-vis titration, ESI-mass, EPR, 1 H NMR titration, and DFT.
Results and DiscussionATA-Msal was gained by the combination of 3-aminothiophene-2-carboxamide and 2-hydroxy-3-methoxybenzaldehyde with 29 % yield in ethanol (Scheme 1). It was characterized by 1 H NMR ( Figure S1), 13 C NMR ( Figure S2) and ESI-mass analysis.