A Novel Fluorescent Method for in situ Hybridization.

Evidence for a putative telomerase repressor gene in the 3p14.2–p21.1 region

“…PCR products were purified by ethanol precipitation and were used as probes. After hybridization of the biotinlabeled probe on normal human metaphase cells, the slides were treated with anti-biotin Fab' alkaline phosphatase conjugate (1:100; Boehringer Mannheim, Indianapolis, IN) for 45 min at 37°C, and hybridization signals were detected by 3-hydroxy-N-2Ј-biphenyl-2-naphthalenecarboxamide phosphate (Boehringer Mannheim) (Kagiyama et al, 1993). Chromosomes were counterstained with quinacrine plus Hoechst 33258 and observed with a Nikon fluorescence microscope.…”

Human endothelin converting enzyme gene (ECE1) mapped to chromosomal region 1p36.1

“…The DNA probe was labeled with digoxigenin-11-dUTP by nick translation. FISH was carried out according to Kagiyama et al (1993). with slight modfications.…”

Assignment of the ATBF1 transcription factor gene (<i>Atbf1</i><i>)</i> to mouse chromosome band 8E1 by in situ hybridization

“…Hybridization of a bio tin-labeled probe was detected with an anti-biotin Fab'-alkalinc phosphatase conjugate, and fluorescent signals were amplified by 3-hydroxy-A'-2'-biphenyl-2-naphthalenecarboxamidc phosphate ester (HNPP) and Fast Red TR (Kagiyama et al, 1993). Chromosomes were stained with Hoechsl 33258 and quinacrine mustard for precise localization of the signals by Q-banding (Kondoh et al.…”