A novel fluorescent probe with a pyrazolo[4,3-<i>c</i>]quinoline core selectively recognizes <i>c-MYC</i> promoter G-quadruplexes

Mohamed, Hany I.; Gao, Chao; Gui, Zhikun; Song, Zibing; Wei, Dengguo

doi:10.1039/d1nj00098e

Cited by 4 publications

(3 citation statements)

References 43 publications

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“…No nucleic acids have been reported to produce fluorophores or to be self-fluorescent . The fluorescence titration data suggested that Sor exhibited a weak fluorescence property.…”

Section: Results and Discussionmentioning

confidence: 99%

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Characterization of the Binding Properties of Sorafenib to c-MYC G-Quadruplexes: Evidence for Screening Potential Ligands

Zeng

et al. 2023

J. Phys. Chem. B

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Sorafenib (Sor) is a multitarget kinase inhibitor used clinically to treat hepatocellular carcinoma and renal cancer. In this study, the interaction mechanism of Sor with c-MYC Gquadruplexes (G4) was investigated at the molecular level by computer-aided means and experiments. Molecular docking results predicted the binding of Sor to the groove of G4. Molecular dynamics (MD) simulations were used to evaluate the effect of ligand binding to G4. Ultraviolet (UV), fluorescence spectroscopy, and viscosity experiments showed that the binding site was in the groove. The UV and fluorescence titration results showed that compared with traditional G4 ligands represented by compound meso-tetra (N-methyl-4-pyridyl) porphine (TmPyP4), Sor has a lower affinity for G4. Likewise, results from fluorescence resonance energy transfer (FRET) experiments suggested that Sor could have a limited ability to stabilize G4, but it was not as prominent as that of TmPyP4. Time-resolved fluorescence spectroscopy again supported the results from steady-state fluorescence spectroscopy, indicating that a static quenching mechanism mainly drove the process. Studying the interaction mechanism of Sor and c-MYC may inspire the screening of new, selective c-MYC G4 ligands and provide ideas for the design of drugs with good stability, low toxicity, and specific targeting of G4.

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“…No nucleic acids have been reported to produce fluorophores or to be self-fluorescent . The fluorescence titration data suggested that Sor exhibited a weak fluorescence property.…”

Section: Results and Discussionmentioning

confidence: 99%

“…No nucleic acids have been reported to produce fluorophores or to be self-fluorescent. 28 The fluorescence titration data suggested that Sor exhibited a weak fluorescence property. It was found that the fluorescence intensity of Sor decreased gradually with the addition of G4 in Figure 4D.…”

Section: Fluorescence Titrationsmentioning

confidence: 99%

Characterization of the Binding Properties of Sorafenib to c-MYC G-Quadruplexes: Evidence for Screening Potential Ligands

Zeng

et al. 2023

J. Phys. Chem. B

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“…20 The quinoline core has gained much attention in the designing of new fluorescent probes as it exhibits a strong binding capability with metal ions and excellent fluorescent properties. [21][22][23] Various quinoline containing probes are reported in the literature 21,24,25 for a variety of applications; 26 taking this into consideration, we chose the quinoline nucleus as the luminescent core and incorporated the ESIPT phenomenon in it for the detection of Cu(II) ions (Fig. 1b) and it is designated as "QHS".…”

Section: Introductionmentioning

confidence: 99%

An ESIPT solvatochromic fluorescent and colorimetric probe for sensitive and selective detection of copper ions in environmental samples and cell lines

Imran

Kaur

Naqvi

et al. 2023

Analyst

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Functional evaluation of novel chromon derivative compounds for recognition of G-quadruplex structure

Neha,

Ranjan,

Kumar

et al. 2024

Journal of Molecular Structure

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A novel fluorescent probe with a pyrazolo[4,3-c]quinoline core selectively recognizes c-MYC promoter G-quadruplexes

Abstract: An aptamer combined with a fluorophore could work as the fluorescent core of nucleic acid mimics of fluorescent proteins to locate and image functional biomolecules in cells. The locating precision...

Cited by 4 publications

References 43 publications

Characterization of the Binding Properties of Sorafenib to c-MYC G-Quadruplexes: Evidence for Screening Potential Ligands

Characterization of the Binding Properties of Sorafenib to c-MYC G-Quadruplexes: Evidence for Screening Potential Ligands

An ESIPT solvatochromic fluorescent and colorimetric probe for sensitive and selective detection of copper ions in environmental samples and cell lines

Functional evaluation of novel chromon derivative compounds for recognition of G-quadruplex structure

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